Effects of Compound Danshen Injection Combined with Magnesium Sulfate on Pregnancy-Induced Hypertension Syndrome under the Guidance of Empirical Mode Decomposition Algorithm-Based Ultrasound Image

Objective. The study focused on the separation effects of ultrasound blood flow signal detection, based on empirical mode decomposition (EMD) algorithm, and the clinical efficacy of Compound Danshen injection and magnesium sulfate in the treatment of pregnancy-induced hypertension (PIH) syndrome. Methods. The empirical mode decomposition (EMD) algorithm was optimized first and compared with other algorithms for the accuracy and stability in separation of blood flow signals. 80 patients with PIH syndrome undergoing ultrasound examination were selected as the research subjects and randomly divided into control group and observation group according to the actual treatment methods. 40 cases in the observation group were treated with Compound Danshen injection + magnesium sulfate, and 40 cases in the control group were treated with magnesium sulfate. After the treatment, the clinical indicators of the two groups of patients were analyzed. Results. The accuracy and stability in separating blood flow signal of the optimized EMD algorithm were better than those of other algorithms. After treatment, the total effective rate and blood pressure control of the observation group were significantly better than those of the control group, and the incidence of adverse maternal and infant outcomes was significantly lower than that of the control group. After treatment, the endothelin-1 (ET-1), C-reactive protein (CRP), and homocysteine (Hcy) indexes of the two groups of patients decreased significantly, and the decrease level of the observation group was significantly greater than that of the control group ( P < 0.05). The prothrombin time (PT), fibrinogen (FIB), activated partial thromboplastin time (APTT), and plasma thrombin time (TT) levels of the two groups after treatment were better than those before treatment, and the observation group was better than the control group ( P < 0.05). Conclusion. The optimized EMD algorithm is of great value for the separation of ultrasound blood flow signals. For patients with PIH syndrome, Compound Danshen injection combined with magnesium sulfate can be used as a treatment plan, which can improve maternal and infant outcomes; control blood pressure; reduce 24 h urine protein and serum ET-1, Hcy, and CRP levels; and improve coagulation function. It is worthy of promotion.

Macromolecular substances as a dangerous factor in traditional Chinese medicine injections were determined by size-exclusive chromatography

Macromolecular substances in traditional Chinese medicine injections (TCMIs) are expected to be a main dangerous factor causing anaphylactic or anaphylactoid reaction. The main aim of the study was to verify the macromolecular substances’ anaphylactic or anaphylactoid reaction in guinea pigs and establish a size-exclusive chromatographic method to detect them. The macromolecular substances from six TCMIs (Danshen injection, Dengzhanxixin injection, Honghua injection, Qingkailing injection, Shuanghuanglian injection and Shuxuening injection) were prepared by removing substances with molecular weight less than 10 kDa with an ultra-filter. The anaphylactic and anaphylactoid reactions caused by original TCMIs, injections rich in or free of macromolecules were assayed in guinea pigs. The relationship between the amount of the macromolecular substances and peak area of chromatogram was established by size-exclusive chromatography. Injections free of macromolecules were not likely to cause anaphylactic and anaphylactoid reactions, but injections rich in macromolecular substances were more likely to do so. If the macromolecular substances with molecular weight bigger than 10 kDa were removed, the signal of macromolecular substances in TCMIs was quantitatively reduced. All the results suggested that macromolecular substances in TCMIs are a dangerous factor causing safety problems, and the macromolecular substances can be quantitatively detected with size-exclusive chromatography.

A Study of Avascular Necrosis of the Femoral Head Using Danshen Combined with Marrow Stromal Cells In Vitro

As avascular necrosis of the femoral head (ANFH) has been one of the major problem plaguing the medical profession and Marrow Stromal Cells (MSCs) in orthopedic exploration for recent years, the aim of this study was to investigate the possible chemotactic effect in vitro treated by Danshen combined with transplantation of MSCs in ANFH. The MSCs were extracted from rabbit tibia and femur. The inhibition rate of Danshen in MSCs proliferation was assayed by MTT. Chemokine receptor expression was determined by RT-PCR. Chemotaxis migration was assayed by Boyden chamber. Results showed that high concentration of Danshen injection on the proliferation of MSCs have a certain inhibition rate. Less than 50 μl/ml did not substantially affect the proliferation of MSCs. After treatment with Danshen injection, it can promote chemokine receptors CCR2, CX3CR1 and CXCR4 expression in MSCs. Danshen could significantly promote cell migration after mixed with 50 ng/ml recombinant monocyte chemoattratant protein-1 (rMCP-1) and recombinant stromal cellderived factor-1 (rSDF-1). In conclusion, culture of MSCs can express chemokine receptors CCR2, CX3CR1 and CXCR4 in vitro. Danshen injection can increase the expression of chemokine receptor CCR2, CX3CR1, and CXCR4, which is beneficial to the migration of MSCs. In conclusion, the chemotactic effects of rMCP-1 and rSDF-1 on the chemotaxis of MSCs were positively correlated with the concentrations of rMCP-1 and rSDF-1 and significantly increased combined with Danshen.