Study of histopathological spectrum of ovarian lesions

Ovarian pathology ranges from innocuous non-neoplastic lesions to malignant neoplastic entities. The incidence, clinical appearance and the behaviour of the different types of ovarian tumour is extremely variable. In order to have a better understanding of frequency and histomorphological patterns of different ovarian lesions study was encountered in our tertiary care hospital.To study the histopathology of ovarian non-neoplastic and neoplastic lesions and to study the distribution of ovarian lesions with respect to various parameters like age, bilaterality, gross and microscopic features.This is a prospective study of 125 ovarian specimens received in department of pathology, G.K. General Hospital, Bhuj during August 1, 2018 to July 31, 2020. Total 125 cases of ovarian specimens were studied, amongst them 58 were non-neoplastic and remaining 67 were neoplastic. The most common non-neoplastic lesion seen was follicular cysts 12 cases (21%), followed by endometriosis 9 cases (16%). Among 67 neoplastic ovarian lesions 58(87%) cases were benign, 3(4%) cases were borderline and 6 (9%) cases were malignant. In benign ovarian neoplasm, most commonly seen lesions were serous cystadenoma 24 cases (36%) followed by 13 cases (20%) of mature cystic teratoma. In malignant cases, maximum was of high-grade serous cystadenocarcinoma.Ovarian epithelial tumours are the most common type, while serous cystadenocarcinoma was the most common malignancy. Histological examination is gold standard and in certain difficult cases require immunohistochemistry.Ovary is an important reproductive organ with involvement in production of progeny.Ovarian pathology ranges from innocuous non-neoplastic lesions to malignant neoplastic entities. Ovarian neoplasms have become increasingly important not only because of the wide range of neoplasms, but also because they have gradually increased the mortality rate. The incidence, clinical appearance and the behaviour of the different types of ovarian tumour is extremely variable.

The circular RNA expression profile in ovarian serous cystadenocarcinoma reveals a complex circRNA–miRNA regulatory network

Background Ovarian serous cystadenocarcinoma is one of the most serious gynecological malignancies. Circular RNA (circRNA) is a type of noncoding RNA with a covalently closed continuous loop structure. Abnormal circRNA expression might be associated with tumorigenesis because of its complex biological mechanisms by, for example, functioning as a microRNA (miRNA) sponge. However, the circRNA expression profile in ovarian serous cystadenocarcinoma and their associations with other RNAs have not yet been characterized. The main purpose of this study was to reveal the circRNA expression profile in ovarian serous cystadenocarcinoma. Methods We collected six specimens from three patients with ovarian serous cystadenocarcinoma and adjacent normal tissues. After RNA sequencing, we analyzed the expression of circRNAs with relevant mRNAs and miRNAs to characterize potential function. Results 15,092 unique circRNAs were identified in six specimens. Approximately 46% of these circRNAs were not recorded in public databases. We then reported 353 differentially expressed circRNAs with oncogenes and tumor-suppressor genes. Furthermore, a conjoint analysis with relevant mRNAs revealed consistent changes between circRNAs and their homologous mRNAs. Overall, construction of a circRNA–miRNA network suggested that 4 special circRNAs could be used as potential biomarkers. Conclusions Our study revealed the circRNA expression profile in the tissues of patients with ovarian serous cystadenocarcinoma. The differential expression of circRNAs was thought to be associated with ovarian serous cystadenocarcinoma in the enrichment analysis, and co-expression analysis with relevant mRNAs and miRNAs illustrated the latent regulatory network. We also constructed a complex circRNA–miRNA interaction network and then demonstrated the potential function of certain circRNAs to aid future diagnosis and treatment.

Potential Roles of Long Non-coding RNAs in Regulating Cell Proliferation, Invasion, and Metastasis in Ovarian Serous Cystadenocarcinoma

Background: Ovarian serous cystadenocarcinoma (OSC) is the most common gynecological malignancy. Long non-coding RNAs (lncRNAs) are aberrantly expressed in many cancers and involved in cell proliferation, apoptosis, angiogenesis, and invasion. Here, we investigated the functional roles of lncRNAs in OSC in detail.Methods: We analyzed a cohort of exon microarray datasets from The Cancer Genome Atlas and used differentially expressed lncRNAs and mRNAs to construct an lncRNA-mRNA co-expression network. Distinct lncRNAs were classified into lincRNA, enhancer-like lncRNAs, or antisense lncRNAs. Biological functions for lncRNAs were predicted according to the lncRNA-mRNA network and genomic adjacency by KEGG pathway analysis. A transcription factor (TF)-lncRNA regulatory network was constructed by integrating lncRNA molecular profiles and TF binding information. Results: We identified 2,939 lncRNAs and 2,766 mRNAs that were differentially expressed between OSC and normal ovary tissues. The 67 lncRNAs in the lncRNA-mRNA network, 23 lincRNAs, 19 antisense lncRNAs, and four enhancer-like lncRNAs were involved in cell proliferation, invasion, and metastasis. The TFs ING4, TTF-1, RUSH-l alpha, Kaiso, and STAT1 targeted regulation of lncRNAs in the pathological processes of OSC. Expression of 10 lncRNAs and mRNAs, as well as SOS1, ITGB1, and BIRC2 mRNAs with their identified lncRNAs were verified by qRT-PCR in OSC tissues. Conclusions: We predicted the biological functions of many lncRNAs, which may serve as diagnostic and prognostic biomarkers as well as therapeutic targets in OSC.