Cu/Fe-Mediated N(sp2)-arylation/alkenylation of pyridines with aryl-/alkenylboronic acids to yield versatile cationic materials

Cu/Fe-mediated arylation of pyridines with arylboronic acids, featuring low-cost catalytic system and easy operation, is disclosed. A series of length-controllable linear (the number of aryls ranges from 2 to 6)...

PEGylation of mRNA by Hybridization of Complementary PEG-RNA Oligonucleotides Stabilizes mRNA without Using Cationic Materials

Messenger RNA (mRNA) delivery strategies are required to protect biologically fragile mRNA from ribonuclease (RNase) attacks to achieve efficient therapeutic protein expression. To tackle this issue, most mRNA delivery systems have used cationic components, which form electrostatically driven complexes with mRNA and shield encapsulated mRNA strands. However, cationic materials interact with anionic biomacromolecules in physiological environments, which leads to unspecific reactions and toxicities. To circumvent this issue of cation-based approaches, herein, we propose a cation-free delivery strategy by hybridization of PEGylated RNA oligonucleotides with mRNA. The PEG strands on the mRNA sterically and electrostatically shielded the mRNA, improving mRNA nuclease stability 15-fold after serum incubation compared with unhybridized mRNA. Eventually, the PEGylated mRNA induced nearly 20-fold higher efficiency of reporter protein expression than unhybridized mRNA in cultured cells. This study provides a platform to establish a safe and efficient cation-free mRNA delivery system.

Replacement of imported long fiber kraft pulp with lignocellulosic nanofibers and cationic materials in the production of durable paper

Usage of cellulosic nanofibers, starch-nanocellulose, and polyacrylamide-nanocellulose hybrid systems were investigated for the replacement of imported long bleached kraft fibers in the production of durable papers. In this study, imported softwood kraft pulp was added to cotton pulp at four levels. Nanofibrillated cellulose (NFC) prepared from chemimechanical pulp was added to cotton pulp at a 5% level with optional 1% cationic starch or 0.1% cationic polyacrylamide. Comparative tests were done without NFC at three levels of either cationic starch or cationic polyacrylamide. For each condition, 80 gm-2 handsheets were made, and the physical, mechanical, and optical properties of the paper were compared. The results showed that increases of NFC yielded higher surface smoothness, tensile strength, resistance to bursting, tearing energy, folding endurance, yellowness, and opacity. It also reduced brightness and porosity whether added singly or in combination with cationic starch or polyacrylamide. Increasing cationic starch also increased surface smoothness, tensile strength, resistance to bursting, and folding endurance, but paper opacity was reduced. The field emission-scanning electron microscopy results showed that increased NFC reduced porosity, the paper surface became smoother, and the pores were relatively filled. Finally, the combined treatment of 5% NFC and 1% cationic starch is introduced as a suitable combination.

Selenium-Epoxy ‘Click’ Reaction and Se-Alkylation—Efficient Access to Organo-Selenium and Selenonium Compounds

This work establishes the ‘click’ nature of the base-catalyzed oxirane ring opening reaction by the selenolate nucleophile. The ‘click’-generated ß-hydroxy selenide can be alkylated to afford cationic selenium species. Hemolytic studies suggest that selenonium cations do not lyse red blood cells even at high concentrations. Overall, these results indicate the future applicability of the developed organo-selenium chemistry in the preparation of a new class of cationic materials based on the seleno-ether motif.

The proper strategy to compress and protect plasmid DNA in the Pluronic L64-electropulse system for enhanced intramuscular gene delivery

Intramuscular expression of functional proteins is a promising strategy for therapeutic purposes. Previously, we developed an intramuscular gene delivery method by combining Pluronic L64 and optimized electropulse, which is among the most efficient methods to date. However, plasmid DNAs (pDNAs) in this method were not compressed, making them unstable and inefficient in vivo. We considered that a proper compression of pDNAs by an appropriate material should facilitate gene expression in this L64-electropulse system. Here, we reported our finding of such a material, Epigallocatechin gallate (EGCG), a natural compound in green teas, which could compress and protect pDNAs and significantly increase intramuscular gene expression in the L64-electropulse system. Meanwhile, we found that polyethylenimine (PEI) could also slightly improve exogenous gene expression in the optimal procedure. By analysing the characteristic differences between EGCG and PEI, we concluded that negatively charged materials with strong affinity to nucleic acids and/or other properties suitable for gene delivery, such as EGCG, are better alternatives than cationic materials (like PEI) for muscle-based gene delivery. The results revealed that a critical principle for material/pDNA complex benefitting intramuscular gene delivery/expression is to keep the complex negatively charged. This proof-of-concept study displays the breakthrough in compressing pDNAs and provides a principle and strategy to develop more efficient intramuscular gene delivery systems for therapeutic applications.

Turf Response to Urease Inhibitors and Cationic Materials Applied in Combination with Urea

We evaluated the response of Kentucky bluegrass (Pea pratensis L.) turf to urea amended with the urease inhibitors PPD, NBPT, and ATS and with the cations K+ (KCl) and Mg+2 (MgCl2). Treatments for the 2-year field experiment included liquid urea applied monthly in June to Sept. 1985 and 1986 at 49 kg N/ha with PPD (1%, 2%, 3% by weight of applied N), NBPT (0.5%, 1%, 2%), ATS (5%, 15%, 25%), K+ (5%, 15%, 25%), and Mg+2 (5%, 15%, 25%). The NBPT was included only in the 1986 field study. The Mg+2 and K+ reduced foliar burn and increased turf quality during mid- and late Summer 1985 at the 5% rate, but clipping yield was not affected by any treatment. In 1986, under milder climatic conditions, PPD and NBPT increased clipping yield by 13.2% and 15.2%, respectively. At the 15% rate, ATS increased clipping yield by 15.1%, but, on average, PPD and NBPT were much more effective. Chemical names used: phenylphosphorodiamidate (PPD), N-(n -butyl) thiophosphoric triamide (NBPT), and ammonium thiosulfate (ATS).