nicotinamide adenine dinucleotide dehydrogenase
Recently Published Documents


TOTAL DOCUMENTS

66
(FIVE YEARS 4)

H-INDEX

15
(FIVE YEARS 0)

Therya ◽  
2021 ◽  
Vol 12 (3) ◽  
pp. 391-409
Author(s):  
Luis Hernán Acosta Salvatierra ◽  
José Luis Poma-Urey ◽  
Paula A Ossa-López ◽  
Fredy A Rivera-Páez ◽  
Héctor E Ramírez-Chaves

Bats of genus Eptesicus are represented in South America by nine species of short-eared taxa (subgenus Eptesicus), and 10 species of long-eared species (subgenus Histiotus).  Here we describe a new species of short-eared Eptesicus based on 19 specimens collected in the sub-Andean Bolivian-Tucumanian forest of Santa Cruz, between 1800-2020 masl.  For this, we include morphological, morphometric, and molecular comparisons; we use principal component, discriminant function and mitochondrial genes (cytochrome-b, cytochrome c oxidase subunit I, and nicotinamide adenine dinucleotide dehydrogenase) to compare the new species with other taxa of the subgenus Eptesicus from South America.  The new species is distinguished from its congeners by cranial shape, body measurements, and genetic distances.  Furthermore, the new species is similar in cranial morphology to Eptesicus andinus but presents a highly developed frontal preorbital process, poorly developed in other related species (i. e., E. andinus, E. furinalis, and E. brasiliensis).  All males were consistently darker than females in the new species.  This taxon increases to 10 the number of species of bats of the subgenus Eptesicus in South America.


2021 ◽  
Author(s):  
REMYA V S ◽  
RESHMI S ◽  
SHEHNA MAHIM S ◽  
ANJALI V R ◽  
ARUNA DEVI C

Abstract 4-Nonylphenol (4-NP) is a breakdown product of nonylphenol ethoxylate (NPE) and attains much concern because of its persistence and toxicity to aquatic organisms. 4-nonylphenol is a known endocrine disruptor and a legendary xenoestrogen. However, non-estrogenic impacts of 4-NP were scarcely studied in aquatic organisms. Hence, the present study evaluated the effects of sublethal concentrations of 4-NP (1.5, 2 and 2.5µl/l) on major carp, Labeo rohita in the intermediary and mitochondrial metabolism. Exposure to 4-NP shows significant changes in the activities of intermediary enzymes like glucose 6 phosphatase, lactate dehydrogenase, cytosolic malic enzyme and isocitrate dehydrogenase and mitochondrial enzymes like malate dehydrogenase, nicotinamide adenine dinucleotide dehydrogenase, succinate dehydrogenase and cytochrome c oxidase when compared to the control (p<0.05). Therefore, the current study indicates that 4-NP at environment concentration impairs the activity of metabolic enzymes and thereby affects the tricarboxylic acid cycle and electron transport system. Alteration in the levels of these parameters can be effectively used to surveil the impact of 4-NP in energy production in aquatic environment.


2021 ◽  
Vol 118 (17) ◽  
pp. e2018538118
Author(s):  
Woon Yong Kwon ◽  
Gil Joon Suh ◽  
Yoon Sun Jung ◽  
Seung Min Park ◽  
Subi Oh ◽  
...  

Secondary infections typically worsen outcomes of patients recovering from septic shock. Neutrophil [polymorphonuclear leukocytes (PMNs)] migration to secondarily inoculated sites may play a key role in inhibiting progression from local bacterial inoculation to secondary infection. Mitochondrial N-formyl peptide (mtFP) occupancy of formyl peptide receptor-1 (FPR1) has been shown to suppress PMN chemotaxis. Therefore, we studied the association between circulating mtFPs and the development of secondary infection in patients with septic shock. We collected clinical data and plasma samples from patients with septic shock admitted to the intensive care unit for longer than 72 h. Impacts of circulating nicotinamide adenine dinucleotide dehydrogenase subunit-6 (ND6) upon clinical outcomes were analyzed. Next, the role of ND6 in PMN chemotaxis was investigated using isolated human PMNs. Studying plasma samples from 97 patients with septic shock, we found that circulating ND6 levels at admission were independently and highly associated with the development of secondary infection (odds ratio = 30.317, 95% CI: 2.904 to 316.407, P = 0.004) and increased 90-d mortality (odds ratio = 1.572, 95% CI: 1.002 to 2.465, P = 0.049). In ex vivo experiments, ND6 pretreatment suppressed FPR1-mediated PMN chemotactic responses to bacterial peptides in the presence of multiple cytokines and chemokines, despite increased nondirectional PMN movements. Circulating mtFPs appear to contribute to the development of secondary infection and increased mortality in patients with septic shock who survive their early hyperinflammatory phase. The increased susceptibility to secondary infection is probably partly mediated by the suppression of FPR1-mediated PMN chemotaxis to secondary infected sites.


Parasitology ◽  
2020 ◽  
pp. 1-13
Author(s):  
Marcelo Tonatiuh González-García ◽  
Leopoldo Andrade-Gómez ◽  
Carlos Daniel Pinacho-Pinacho ◽  
Ana Lucia Sereno-Uribe ◽  
Martín García-Varela

Abstract Saccocoelioides is a genus of trematodes associated with fishes from the Americas. In the current research, morphologically distinct specimens of Saccocoelioides spp. were collected from six countries in Middle America. Specimens were sequenced using three molecular markers, the domains D1–D3 of the large subunit (LSU) from the nuclear rDNA, the cytochrome c oxidase subunit 1 (cox1) and nicotinamide adenine dinucleotide dehydrogenase subunit 1 (nad1) from mitochondrial DNA. A total of 74 new sequences were compared and aligned with other sequences available in GenBank. Maximum likelihood and Bayesian inference analyses were inferred from the LSU and cox1 datasets, revealing unequivocally that all the specimens correspond to S. lamothei. A haplotype network was built with 119 sequences of the nad1 gene. The network detected 57 distinct haplotypes divided into three haplogroups. To explore morphological differences among samples of S. lamothei, 17 morphological features were measured from 53 specimens from three fish families: Eleotridae, Mugilidae and Gobiidae. Principal component analysis yielded three main polygons that corresponded with each family analysed, suggesting host-induced phenotypic plasticity. The current evidence suggests that S. lamothei infects at least five fish families along the Pacific coasts of Mexico, Guatemala, El Salvador, Honduras, Nicaragua and Costa Rica.


2020 ◽  
Vol 58 (5) ◽  
pp. 499-511
Author(s):  
Preeyaporn Butboonchoo ◽  
Chalobol Wongsawad ◽  
Pheravut Wongsawad ◽  
Jong-Yil Chai

Echinostome metacercariae were investigated in freshwater snails from 26 districts in 7 provinces of upper northern Thailand. The species identification was carried out based on the morphologies of the metacercariae and adult flukes harvested from experimental hamsters, and on nucleotide sequences of internal transcribed spacer 2 (ITS2) and nicotinamide adenine dinucleotide dehydrogenase subunit 1 (nad1) genes. Twenty-four out of 26 districts were found to be infected with echinostome metacercariae in freshwater snails with the prevalence of 40.4%. The metacercariae were found in all 6 species of snails, including Filopaludina martensi martensi (21.9%), Filopaludina doliaris (50.8%), F. sumatrensis polygramma (61.3%), Bithynia siamensis siamensis (14.5%), Bithynia pulchella (38.0%), and Anenthome helena (4.9%). The echinostome metacercariae found in these snails were identified as Echinostoma revolutum (37-collar-spined) and Echinostoma macrorchis (45-collar-spined) morphologically and molecularly. The 2-week-old adult flukes of E. revolutum revealed unique features of the cirrus sac extending to middle of the ventral sucker and smooth testes. E. macrorchis adults revealed the cirrus sac close to the right lateral margin of the ventral sucker and 2 large and elliptical testes with slight indentations and pointed posterior end of the posterior testis. The ITS2 and nad1 sequences confirmed the species identification of E. revolutum, and the sequences of E. macrorchis have been deposited for the first time in Gen-Bank. The presence of the life cycle of E. macrorchis is a new record in Thailand and the snail F. doliaris as their second intermediate host seems to be new among the literature.


2020 ◽  
Vol 8 (1) ◽  
pp. e001372
Author(s):  
Mihae Seo ◽  
Hyoungnae Kim ◽  
Hyunjin Noh ◽  
Jin Seok Jeon ◽  
Dong Won Byun ◽  
...  

IntroductionRecent studies have suggested that extracellular circulating and urinary mitochondrial DNA (mtDNA) are associated with mitochondrial dysfunction in obesity and type 2 diabetes mellitus (T2DM). However, the changes to cell-free serum and urinary mtDNA after bariatric surgery in patients with obesity with T2DM have not been investigated to date.Research design and methodsWe prospectively recruited patients with obesity (n=18), and with obesity and T2DM (n=14) who underwent bariatric surgery, along with healthy volunteers (HV) as a control group (n=22). Serum and urinary mitochondrial nicotinamide adenine dinucleotide dehydrogenase subunit-1 (mtND-1) and cytochrome-c oxidase 3 (mtCOX-3) copy numbers were measured using quantitative PCR (qPCR). The mtDNA copy numbers of patients with obesity (with and without T2DM) were followed up 6 months after surgery.ResultsThe copy numbers of urinary mtND-1 and mtCOX-3 in patients with obesity, with or without T2DM, were higher than those in the HVs. Moreover, urinary mtCOX-3 copy number increased in patients with obesity with T2DM compared with patients with obesity without T2DM (p=0.018). Meanwhile, serum mtCOX-3 copy numbers in HV were higher in both obesity patient groups (p=0.040). Bariatric surgery reduced urinary mtND-1 and mtCOX-3 copy numbers, as well as serum mtCOX-3 copy numbers only in patients with obesity with T2DM.ConclusionThese results suggest that T2DM induces greater kidney mitochondrial dysfunction in patients with obesity, which can be effectively restored with bariatric surgery.


Parasitology ◽  
2020 ◽  
Vol 147 (14) ◽  
pp. 1723-1727
Author(s):  
A Rojas ◽  
H Salant ◽  
D Yasur-Landau ◽  
H Tsarfati ◽  
G Baneth

AbstractOnchocerca lupi is a parasitic filarioid and the causative agent of canine ocular onchocercosis, a zoonotic disease of domestic dogs with sporadic reports in humans. A 13-year-old dog with no travel history outside of Israel was presented to an ophthalmology veterinary clinic in Israel with severe right ocular and periocular disease. After surgical exploration, thin helminths were removed from the dorsal sclera of the eye and identified as Onchocerca lupi by polymerase chain reaction according to the cytochrome c oxidase subunit I (cox1), reduced nicotinamide adenine dinucleotide dehydrogenase subunit 5 (nad5) and 12S rRNA genes. Phylogenetic trees and haplotype networks of the cox1 and nad5 genes confirmed the circulation of two genotypes: genotype 1 with worms from dogs, cats and humans from both the Old and New Worlds, and genotype 2 with specimens from Portugal and Spain. The Israeli sequences clustered in genotype 1 and were identical to O. lupi from the USA. Evidence of two genotypes separated geographically sheds light on the phylogeography and evolution of this zoonotic pathogen, and suggests a diverse pathology observed in different regions of the world.


Parasitology ◽  
2019 ◽  
Vol 147 (3) ◽  
pp. 371-375 ◽  
Author(s):  
María Ornela Beltrame ◽  
Cesar Pruzzo ◽  
Rodrigo Sanabria ◽  
Alberto Pérez ◽  
Matías Sebastián Mora

AbstractIt is generally assumed that the digenean human liver fluke, Fasciola hepatica, gained entry to South America during the 15th century upon arrival of Europeans and their livestock. Nonetheless in Patagonia, Argentina, digenean eggs similar to F. hepatica have been observed in deer coprolites dating back to 2300 years B.P. The main objective of our present study was to identify and characterize these eggs using an ancient DNA (aDNA) study. Eggs were isolated and used for aDNA extraction, amplification and sequencing of partial regions from the cytochrome c oxidase subunit 1 and the nicotinamide adenine dinucleotide dehydrogenase subunit 1 mitochondrial genes. Also, phylogenetic trees were constructed using Bayesian and maximum likelihood. Our results confirm the presence of F. hepatica in South America from at least 2300 years B.P. This is the first report and the first aDNA study of this trematode in South America prior to the arrival of the European cattle in the 15th century. The present work contributes to the study of phylogenetic and palaeobiogeographical aspects of F. hepatica and its settlement across America.


2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Byung Chul Yu ◽  
Nam-Jun Cho ◽  
Samel Park ◽  
Hyoungnae Kim ◽  
Soo Jeong Choi ◽  
...  

Abstract Mitochondrial injury plays important roles in the pathogenesis of various kidney diseases. However, mitochondrial injury in IgA nephropathy (IgAN) remains largely unexplored. Here, we examined the associations among mitochondrial injury, IgAN, and treatment outcomes. We prospectively enrolled patients with IgAN and age-/sex-matched healthy volunteers (HVs) as controls (n = 31 each). Urinary copy numbers of the mitochondrial DNA (mtDNA) genes cytochrome-c oxidase-3 (COX3) and nicotinamide adenine dinucleotide dehydrogenase subunit-1 (ND1) were measured. Urinary mtDNA levels were elevated in the IgAN group compared with that in HVs (p < 0.001). Urinary ND1 levels were significantly higher in the low proteinuria group than in the high proteinuria group (p = 0.027). Changes in urinary levels of ND1 and COX3 were positively correlated with changes in proteinuria (p = 0.038 and 0.024, respectively) and inversely correlated with changes in the estimated glomerular filtration rate (p = 0.033 and 0.017, respectively) after medical treatment. Mitochondrial injury played important roles in IgAN pathogenesis and may be involved in early-stage glomerular inflammation, prior to pathological changes and increased proteinuria. The correlation between changes in urinary mtDNA and proteinuria suggest that these factors may be promising biomarkers for treatment outcomes in IgAN.


Sign in / Sign up

Export Citation Format

Share Document