Immunodiagnosis and Immunotherapeutics Based on Human Papillomavirus for HPV-Induced Cancers

Infection with human papillomavirus (HPV) is one of the main causes of malignant neoplasms, especially cervical, anogenital, and oropharyngeal cancers. Although we have developed preventive vaccines that can protect from HPV infection, there are still many new cases of HPV-related cancers worldwide. Early diagnosis and therapy are therefore important for the treatment of these diseases. As HPVs are the major contributors to these cancers, it is reasonable to develop reagents, kits, or devices to detect and eliminate HPVs for early diagnosis and therapeutics. Immunological methods are precise strategies that are promising for the accurate detection and blockade of HPVs. During the last decades, the mechanism of how HPVs induce neoplasms has been extensively elucidated, and several oncogenic HPV early proteins, including E5, E6, and E7, have been shown to be positively related to the oncogenesis and malignancy of HPV-induced cancers. These oncoproteins are promising biomarkers for diagnosis and as targets for the therapeutics of HPV-related cancers. Importantly, many specific monoclonal antibodies (mAbs), or newly designed antibody mimics, as well as new immunological kits, devices, and reagents have been developed for both the immunodiagnosis and immunotherapeutics of HPV-induced cancers. In the current review, we summarize the research progress in the immunodiagnosis and immunotherapeutics based on HPV for HPV-induced cancers. In particular, we depict the most promising serological methods for the detection of HPV infection and several therapeutical immunotherapeutics based on HPV, using immunological tools, including native mAbs, radio-labelled mAbs, affitoxins (affibody-linked toxins), intracellular single-chain antibodies (scFvs), nanobodies, therapeutical vaccines, and T-cell-based therapies. Our review aims to provide new clues for researchers to develop novel strategies and methods for the diagnosis and treatment of HPV-induced tumors.

Molecularly Imprinted Electrochemical Sensors: Analytical and Pharmaceutical Applications Based on Ortho-Phenylenediamine Polymerization

Background: The molecular imprinting technique has been applied in many fields including separation, artificial antibody mimics, catalysis, sensing studies, and drug delivery. The reasons for the popularity of this technique among the researchers are high selectivity due to the cavities that are formed on the polymer surface for the specific analyte, high robustness, high durability under extreme conditions and low cost. When these advantages are combined with the advantages of electrochemical methods such as rapid response time, ease of use, cheapness and miniaturizability, Molecularly Imprinted Polymer (MIP) based electrochemical sensors turn out to be a widely-preferred sensing tool. Objective: This article provides the reader with information on MIP-based electrochemical sensors and reviews the applications of the MIP sensors prepared by electropolymerization of orthophenylenediamine, a monomer whose mechanical and chemical stability is very high. Results and Conclusion: The literature survey summarized in this review shows that cyclic voltammetry is the most widely preferred electrochemical technique for electropolymerization of o-PD. The media chosen is generally acetate or phosphate buffers with different pH values. Although there are numerous solvents used for template removal, generally methanol and NaOH have been chosen.