Phylogeography of the longnose dace (Rhinichthys cataractae) species group in northwestern North America — the Nooksack dace problem

The longnose dace (Rhinichthys cataractae (Valenciennes, 1842); Cyprinidae) is one of the most widespread freshwater fishes in North America, and across its range there have been several divergent forms described that are of uncertain taxonomic status. One of these forms, the Nooksack dace, is found in southwestern British Columbia and adjacent portions of western Washington, and is distinguished from longnose dace by a lower number of lateral-line scales. We sequenced a total of approximately 1400 base pairs (bp) of mitochondrial DNA (mtDNA) and noted that the longnose dace found west of the Continental Divide and Nooksack dace constituted reciprocally monophyletic clades that differed from each other by between 2% and 3% sequence divergence. Sequence analysis at two nuclear loci (the S7 ribosomal protein intron 1 (S7) and recombination activation gene 1 (RAG1)), however, showed no consistent difference between longnose dace and Nooksack dace and several alleles were shared between them. By contrast, consistent differences at both mtDNA and nuclear DNA loci were resolved between R. cataractae samples from east and west of the Continental Divide. The Nooksack dace does not appear to warrant separate taxonomic status from the longnose dace, but the mtDNA differences support its recognition as an important component of the evolutionary and biogeographic legacy of R. cataractae.

Hydrological drought and the role of refugia in an endangered riffle-dwelling fish, Nooksack dace (Rhinichthys cataractae ssp.)

Understanding the impacts of hydrological drought, and the role that refugia play in mitigating these impacts, is crucial to the conservation of freshwater fishes. This is especially true for species adapted to riffles, which are typically the first habitats to dewater at low discharge. We examined the relationship among decreasing stream discharge, abundance, and habitat use for Nooksack dace (Rhinichthys cataractae ssp.), an endangered riffle-dwelling species. A complementary experimental manipulation examined the effects of flow on growth rate across a discharge gradient in riffle and pool habitats. We found that low-velocity habitats and decreased discharge in experimental channels result in reduced dace growth and that decreasing stream flow was coincident with declines in Nooksack dace abundance. This study demonstrates the sensitivity of Nooksack dace to hydrological drought, and insofar as Nooksack dace are ecologically typical of small riffle-dwelling invertivore fishes, our results suggest that use of pools does not mitigate sublethal effects of declining flows on growth, although pools may provide refuge from the most negative effects of drought (i.e., stranding of fish).