ВИДОВИЙ СКЛАД ТА СТАН ПОПУЛЯЦІЙ ЛІКАРСЬКИХ РОСЛИН НА ТЕРИТО-РІЇ ЛУГАНСЬКОЇ ОБЛАСТІ

Наведено результати дослі-дження фітоценозів Луганської області з метою встановлення видового складу лікарських рослин та стану популяцій. Ідентифіковано 304 види. Малочисельними популяціями харак-теризувалися на луках Dracocephalum triflorum L., Stachys palustris L., Tussilago farfara L., Rhaponticum serratuloides (Georgi) Bobrov, Bidens tripartitа L.; на лісових галявинах – Platan-thera bifolia (L.) Rich., Veronica officinalis L., Symphytum officinale L., Vincetoxicum albovianum (Kusn.) Pobed., Hypericum perforatum L., Polygonatum officinale All., Hierochloe odorata Wahid; по берегах водойм – Acorus calamus L., Stratiotes aloides L., Gratiola officinalis L., Iris pseudacorus L.Викликає занепокоєння малочисельність таких видів лікарських рослин, як Veronica officinalis, Iris pseudacorus, Symphytum officinale, Tussilago farfara, які потребують збереження та відтворення.

Promising plant species for reducing bird populations at airfields

For crops and landings on the territory of airfields in order to reduce the population of birds, promising: Limus (Leymus arenarius), gray's Sedge (Carex grayi), Melica (Melica altissima), bison sweet (Hierochloe odorata), Japanese Spiraea (Spiraea japonica) and species of dogwood (Cotoneaster sp.).

Redox Properties of Novel Antioxidant 5,8-Dihydroxycoumarin: Implications for its Prooxidant Cytotoxicity

The aim of this work was to characterize the redox properties of the new antioxidant 5,8- dihydroxycoumarin (5,8-DHC), isolated from sweet grass (Hierochloë odorata L.), and to determine its impact on its cytotoxic action. Reversible electrochemical oxidation of 5,8- DHC at pH 7.0 was characterized by the midpoint potential (Ep/2) of 0.23 V vs. the normal hydrogen electrode. 5,8-DHC was slowly autoxidized at pH 7.0, and it was active as a substrate for peroxidase (POD, EC 1.11.1.7) and tyrosinase (TYR, EC 1.14.18.1). Oxidation of 5,8-DHC by POD/H2O2 yielded the product(s) which reacted with reduced glutathione and supported the oxidation of NADPH by ferredoxin:NADP+ reductase (FNR, EC 1.18.1.2) and NAD(P)H:quinone oxidoreductase (NQO1, DT-diaphorase, EC 1.6.99.2). The concentration of 5,8-DHC for 50% survival of bovine leukemia virus-transformed lamb kidney fibroblasts (line FLK) during a 24-h incubation was (60 ± 5.5) μm. Cytotoxicity of 5,8-DHC was decreased by desferrioxamine, catalase, the antioxidant N,N’-diphenyl-p-phenylene diamine, and potentiated by 1,3-bis-(2-chloroethyl)-1-nitrosourea and dicumarol, an inhibitor of NQO1. This shows that 5,8-DHC possesses the oxidative stress-type cytotoxicity, evidently due to the action of quinodal oxidation product(s). The protective effect of isoniazide, an inhibitor of cytochrome P-450 2E1, points to hydroxylation of 5,8-DHC as additional toxification route, whereas the potentiating effect of 3,5-dinitrocatechol, an inhibitor of catechol-omethyltransferase (COMT, EC 2.1.1.6), points to the o-methylation of hydroxylation products as the detoxification route.