vdac channels
Recently Published Documents


TOTAL DOCUMENTS

11
(FIVE YEARS 0)

H-INDEX

11
(FIVE YEARS 0)

2007 ◽  
Vol 292 (4) ◽  
pp. C1388-C1397 ◽  
Author(s):  
Wenzhi Tan ◽  
Johnathan C. Lai ◽  
Paul Miller ◽  
C. A. Stein ◽  
Marco Colombini

G3139, an antisense Bcl-2 phosphorothioate oligodeoxyribonucleotide, induces apoptosis in melanoma and other cancer cells. This apoptosis happens before and in the absence of the downregulation of Bcl-2 and thus seems to be Bcl-2-independent. Binding of G3139 to mitochondria and its ability to close voltage-dependent anion-selective channel (VDAC) have led to the hypothesis that G3139 acts, in part, by interacting with VDAC channels in the mitochondrial outer membrane ( 21 ). In this study, we demonstrate that G3139 is able to reduce the mitochondrial outer membrane permeability to ADP by a factor of 6 or 7 with a Ki between 0.2 and 0.5 μM. Because VDAC is responsible for this permeability, this result strengthens the aforesaid hypothesis. Other mitochondrial respiration components are not affected by [G3139] up to 1 μM. Higher levels begin to inhibit respiration rates, decrease light scattering and increase uncoupled respiration. These results agree with accumulating evidence that VDAC closure favors cytochrome c release. The speed of this effect (within 10 min) places it early in the apoptotic cascade with cytochrome c release occurring at later times. Other phosphorothioate oligonucleotides are also able to induce VDAC closure, and there is some length dependence. The phosphorothioate linkages are required to induce the reduction of outer membrane permeability. At levels below 1 μM, phosphorothioate oligonucleotides are the first specific tools to restrict mitochondrial outer membrane permeability.


2004 ◽  
Vol 279 (14) ◽  
pp. 13575-13583 ◽  
Author(s):  
Tatiana K. Rostovtseva ◽  
Bruno Antonsson ◽  
Motoshi Suzuki ◽  
Richard J. Youle ◽  
Marco Colombini ◽  
...  
Keyword(s):  

2002 ◽  
Vol 187 (2) ◽  
pp. 147-156 ◽  
Author(s):  
T.K. Rostovtseva ◽  
A. Komarov ◽  
S.M. Bezrukov ◽  
M. Colombini

2002 ◽  
Vol 82 (1) ◽  
pp. 193-205 ◽  
Author(s):  
Tatiana K. Rostovtseva ◽  
Alexander Komarov ◽  
Sergey M. Bezrukov ◽  
Marco Colombini

IUBMB Life ◽  
2001 ◽  
Vol 52 (3-5) ◽  
pp. 113-118 ◽  
Author(s):  
Michael Forte ◽  
Elizabeth Blachly-Dyson
Keyword(s):  

1997 ◽  
Vol 157 (3) ◽  
pp. 271-279 ◽  
Author(s):  
T. Hodge ◽  
M. Colombini
Keyword(s):  

Sign in / Sign up

Export Citation Format

Share Document