Differential sampling of AC waveforms based on a programmable Josephson voltage standard using a high-precision sampler

A high-precision sampler, Fluke 8588A multimeter in the sampling mode, was utilised to perform differential sampling of AC waveforms with a programmable Josephson voltage standard. The systematic error on the differential sampling, induced by the inherent voltage-response characteristics and built-in low-pass filter of the sampler, was estimated. Experimental results and numerical simulations revealed that the sampler could be used for reliable differential sampling of AC waveforms at frequencies up to several kilohertz, with an appropriate number of the voltage steps per the waveform period, when the input bandwidth was set to 3 MHz. In addition, the sampler was compared to an integrating sampler, Keysight 3458A, now widely used for differential sampling. At 62.5 Hz, a key frequency in the future on-site key comparison of the differential sampling on AC voltage, the difference in RMS-amplitude values obtained by the differential sampling using the two different samplers is approximately 150 nV/V due to the systematic error caused by the limited bandwidth of 150~kHz for the integrating sampler.

A new generalized cross-connected switched capacitor multilevel inverter topology with high output voltage gain for single phase solar PV unit

Switched capacitor multilevel inverters are gaining much attention these days due to their merits like voltage boosting and voltage balancing characteristics. A Cross Connected Switched Capacitor Multilevel Inverter (C2SC-MLI) topology is proposed in this work. It can synthesize thirteen levels in the terminal voltage waveform and with a voltage boost ratio of 1:3. The topology is extendable by adding additional “n” modules in series. The number of levels (NLevel) and the voltage gain can be increased up to 4i+9 and 1:(i+2) respectively by connecting ‘i’ such ‘n’ modules. The topology also has inherent voltage balancing ability. To prove the advantage of proposed topology it is compared with recent switched capacitor multilevel inverter topologies and conventional multilevel inverter topologies in terms of number of power electronic components required, cost and voltage gain. The performance of proposed topology is validated using simulation in MATLAB and with an experimental prototype rated 0.1 kW fed by a solar PV emulator under steady state and dynamic loading conditions.

A Three-Phase Resonant Boost Inverter Fed Brushless DC Motor Drive for Electric Vehicles

The present article proposes a three-phase resonant boost inverter (TPRBI) to feed a permanent magnet brushless DC (PMBLDC) motor at the requested torque with low ripples due to the sinusoidal current injected into the PMBLDC motor. PMBLDC motors have the highest torque-to-weight ratio compared to other motors and are the best choice for electric vehicle applications. Conventionally, these motors are driven by voltage source inverters (VSI) with trapezoidal current injection, introducing unwanted torque ripples. Moreover, due to the buck operation of VSI, an extra power conversion stage is required to elevate the battery voltage level to desired DC-link voltage. This extra stage increases the number of components used, complexity of control and decreases the efficiency and reliability of the overall system. TPRBI injects sinusoidal current in the PMBLDC motor in the proposed method, thus minimizing the torque ripples. The proposed inverter also has an inherent voltage boost characteristic, thus eliminating the extra power conversion stage. The single-stage conversion from DC to boosted sinusoidal AC enhances the system reliability and efficiency and minimizes the cost and weight of the system. A MATLAB/Simulink model is presented along with simulation results and mathematical validation. A comparative evaluation of the proposed system with the conventional VSI-fed PMBLDC motor is presented in terms of induced torque ripples.

Voltage-independent GluN2A-type NMDA receptor Ca2+ signaling promotes audiogenic seizures, attentional and cognitive deficits in mice

The NMDA receptor-mediated Ca2+ signaling during simultaneous pre- and postsynaptic activity is critically involved in synaptic plasticity and thus has a key role in the nervous system. In GRIN2-variant patients alterations of this coincidence detection provoked complex clinical phenotypes, ranging from reduced muscle strength to epileptic seizures and intellectual disability. By using our gene-targeted mouse line (Grin2aN615S), we show that voltage-independent glutamate-gated signaling of GluN2A-containing NMDA receptors is associated with NMDAR-dependent audiogenic seizures due to hyperexcitable midbrain circuits. In contrast, the NMDAR antagonist MK-801-induced c-Fos expression is reduced in the hippocampus. Likewise, the synchronization of theta- and gamma oscillatory activity is lowered during exploration, demonstrating reduced hippocampal activity. This is associated with exploratory hyperactivity and aberrantly increased and dysregulated levels of attention that can interfere with associative learning, in particular when relevant cues and reward outcomes are disconnected in space and time. Together, our findings provide (i) experimental evidence that the inherent voltage-dependent Ca2+ signaling of NMDA receptors is essential for maintaining appropriate responses to sensory stimuli and (ii) a mechanistic explanation for the neurological manifestations seen in the NMDAR-related human disorders with GRIN2 variant-meidiated intellectual disability and focal epilepsy.

Mutations reveal voltage gating of CNGA1 channels in saturating cGMP

Activity of cyclic nucleotide–gated (CNG) cation channels underlies signal transduction in vertebrate visual receptors. These highly specialized receptor channels open when they bind cyclic GMP (cGMP). Here, we find that certain mutations restricted to the region around the ion selectivity filter render the channels essentially fully voltage gated, in such a manner that the channels remain mostly closed at physiological voltages, even in the presence of saturating concentrations of cGMP. This voltage-dependent gating resembles the selectivity filter-based mechanism seen in KcsA K+ channels, not the S4-based mechanism of voltage-gated K+ channels. Mutations that render CNG channels gated by voltage loosen the attachment of the selectivity filter to its surrounding structure, thereby shifting the channel's gating equilibrium toward closed conformations. Significant pore opening in mutant channels occurs only when positive voltages drive the pore from a low-probability open conformation toward a second open conformation to increase the channels' open probability. Thus, the structure surrounding the selectivity filter has evolved to (nearly completely) suppress the expression of inherent voltage-dependent gating of CNGA1, ensuring that the binding of cGMP by itself suffices to open the channels at physiological voltages.