Discovery of novel compounds as potent activators of Sirt3

Among the sirtuin members, Sirt3 is one of the most important deacetylases as it regulates acetylation levels in mitochondria, which are linked to the metabolism of multiple organs and therefore involved in many types of human diseases such as age-related diseases, cancer, heart disease and metabolic diseases. In the current absence of any direct activator of Sirt3, the identification of new modulators could be a key step in the development of new therapeutics. Here we report the discovery of Sirt3 modulators thanks to DNA encoded library technology (ELT). The most enriched compounds after DEL selection against SIRT3 were evaluated according to their activity and affinity. Our best activator seems at least as potent as Honokiol (HKL) while the docking studies tend to show that our modulators probably interact with Sirt3 at an atypical site.

Public Libraries Help Patrons of Color to Bridge the Digital Divide, but Barriers Remain

A Review of: Pun, R. (2021). Understanding the roles of public libraries and digital exclusion through critical race theory: An exploratory study of people of color in California affected by the digital divide and the pandemic. Urban Library Journal, 26(2). https://academicworks.cuny.edu/ulj/vol26/iss2/1/ Abstract Objective – This study explored the role of the public library in the support of patrons of color who experience digital exclusion. Design – In-person and telephone interviews, grounded theory, and critical race theory. Setting – Public libraries in California. Subjects – Persons of color who were active public library technology resource users due to experiencing the digital divide. Methods – In-person, 60- to 90-minute interviews were conducted with participants referred to the author by public librarians at select libraries in California. Sixteen open-ended questions were asked, relating to demographics, access to technology at home, library technology access and use, technology skills, and thoughts on how libraries could change or improve technology services. A 20- to 30-minute follow-up interview was conducted during the phase of the Covid-19 pandemic when public libraries were closed. Interview transcripts were analyzed by the author, who created a codebook of common themes. Responses were analyzed through the lens of grounded theory and critical race theory. Main Results – Nine participants were recruited; six consented to the first interview and two of the six consented to the second interview. Four of the participants self-reported as Asian, one as Black/African American, and one as Hispanic/Latino American. None of the participants had internet access in their homes, though some reported having laptops or inconsistent cellular service. Common uses of library technology included job search activities (resume building, job searching, applications); schoolwork; research and skill development; and legal or housing form finding. Leisure activities including social media and YouTube were also mentioned. Access limitations included inconvenient library hours, particularly for those attending college or holding a job with daytime hours, and physical distance from the library. A common complaint was the time limit on computer access set by the library; “the concept of time” was mentioned “over 70 times collectively by all participants” (p. 14). Language was another barrier to access, mentioned by three of the participants. Most reported being more likely to ask for help from a library staff person who shared their language or had a similar background. Participants also reported wishing more technology workshops were offered, especially workshops in languages other than English. The two participants who took part in the second interview “expressed frustration and sadness” about the lack of library access during the Covid-19 pandemic (p. 16). One participant reported having to get internet access at her home for her children to attend school. The second participant expressed her difficulty in conducting research or printing information with only the small screen of her phone to provide access. Conclusion – Library patrons of color living within the digital divide make use of public library technology but experience multiple barriers. Libraries can alleviate these barriers by examining their hours, policies, and staffing models to be more accessible to patrons of color lacking internet access at home.

Sharing electronically and accessibly in library-led instruction

The electronic information and technology accessibility project is a strategic overhauling of the digital instructional materials of the Health Sciences Library System (HSLS) to comply with the accessibility standards established in a 2020 University of Pittsburgh policy. Though these technologies have existed for quite some time, library instructors were not skilled in the actual creation and design of documents, web content, and presentations with accessibility in mind. Over the past year and a half, a team within HSLS developed detailed guidance and education on universal design and creating an inclusive online learning environment. These guidelines were developed in accordance with Section 508 and the WCAG2.1, with a focus on an improved experience for the D/deaf community and those with visual impairments. We initially made accessibility improvements to online subject guides, in-person presentations, and digitally shared class materials. The COVID-19 pandemic and complete shift to virtual instruction then necessitated the evaluation of platforms used in remote learning (such as Zoom and Panopto), where accessibility best practices needed to be incorporated. This article highlights going beyond in-program accessibility checkers and describes how library technology experts and content creators worked together to bridge the gap of accessibility in the information we share.

Experiences of Library Professionals on DSpace Installation

DSpace, a free and open-source software (FOSS) provides the digital platform for building collections of an institution. Due to the lack of the skills to install and configure DSpace, librarians are incapable to conduct it even after the awareness about it. Identifying challenges about DSpace installation is vital to increase its use by librarians. Hence, it aims to explore the understanding of DSpace installation among librarians and to identify aspects of their experiences and feelings while installing DSpace. Librarians' experiences and feelings are important for accepting the software. Librarians were interviewed during the training program conducted on digital library technology from December 2020 to January 2021. Two groups were distinguishing from each other: one is with a library science background and another is with computer science and engineering background. The second group aswas self-sustained to install DSpace, once they got the guidelines for installation. Their views were reflected with the code terms status, exploration, feeling, activities, preferences, and inadequacy. Through their experiences, the aspects of challenges are the less experience of using the command line in computer use, the unavailability of teaching courses on Linux operating system in Library and Information Science (LIS) education, and the lack of self-exploration habits. Practice to Linux commands, incorporate the Linux in the Library Science course, self-exploration, and consistent effort will help librarians in installing DSpace by themselves. Finally, librarians should internalize their goal to act as the administrator for the DSpace, not only as of the operator.

The trajectory of linked data in late capitalism

PurposeThe aim of this article is to analyze the trajectory of library linked data in light of the ideological machinations of late capitalism. This is accomplished in order to understand how and why its development currently occupies a place of contradiction and provide avenues for examining how this tension can be resolved.Design/methodology/approachOur approach relies on the work of Boltanski and Chiapello's “new spirit of capitalism” to understand the rise of the network and situate linked data within that history by examining various technologies, projects and agents involved in its development. We use this history to outline the growth of contradictory tensions within linked data necessitated by capitalistic growth.FindingsLibrary linked data has found itself in a contradictory position because of the nature of late capitalist expansion, but this development has been facilitated largely by hegemonic agents within libraries and related institutions. We suggest that a counter-hegemonic lens be applied to envisioning linked data's future and its infrastructures.Originality/valueTo our knowledge this article represents one of the first attempts to provide a critique of late capitalist designs on linked data with a particular emphasis on hegemonic control over library technology and infrastructures.

Multiplex viral tropism assay in complex cell populations with single-cell resolution

Gene therapy constitutes one of the most promising modes of disease treatments. Two key properties for therapeutic delivery vectors are the transduction efficiency (how well the vector delivers therapeutic cargo to desired target cells) and specificity (how well it avoids off-target delivery into the other unintended cells within the body). Here we developed a novel technology that enables multiplex measurement of transduction efficiency and specificity, particularly by measuring how libraries of delivery vectors transduce libraries of diverse cell types. We demonstrated that pairing high-throughput measurement of AAV identity with high-resolution single-cell RNA transcriptomic sequencing maps how natural and engineered AAV variants transduce individual cells within human cerebral and ocular organoids. This library-on-library technology is important for determining the safety and efficacy of therapeutic delivery vectors.