soil dna extraction
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BIO-PROTOCOL ◽  
2020 ◽  
Vol 10 (2) ◽  
Author(s):  
Asmita Kamble ◽  
Harinder Singh

Protist ◽  
2017 ◽  
Vol 168 (3) ◽  
pp. 283-293 ◽  
Author(s):  
Susana S. Santos ◽  
Inês Nunes ◽  
Tue K. Nielsen ◽  
Samuel Jacquiod ◽  
Lars H. Hansen ◽  
...  

2015 ◽  
Vol 11 (2) ◽  
pp. 268-272 ◽  
Author(s):  
Jennifer M. Young ◽  
Laura S. Weyrich ◽  
Laurence J. Clarke ◽  
Alan Cooper

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
Faria Fatima ◽  
Neelam Pathak ◽  
Smita Rastogi Verma

The need for identification of soil microbial community mainly depends on direct extraction of DNA from soil, a multifaceted environment that is a major pool for microbial genetic diversity. The soil DNA extraction procedures usually suffer from two major problems, namely, inappropriate rupturing of cells and contamination with humic substances. In the present study, five protocols for single type of rhizospheric soil were investigated and their comparison indicated that the inclusion of 120 mM phosphate buffered saline (PBS) for washing and mannitol in the lysis buffer allowed the processing of soil sample in minimal time with no specific equipment requirement. Furthermore, DNA purity and yield were also improved, which allowed the exploitation of genetic potential of soil microbes within soil sample thereby facilitating the amplification of metagenomic DNA. The effectiveness of methods was analyzed using random amplification of polymorphic DNA. The banding patterns revealed that both the abundance and the composition of indigenous microbial community depend on the DNA recovery method.


2012 ◽  
Vol 134 (3) ◽  
pp. 467-473 ◽  
Author(s):  
James W. Woodhall ◽  
Kathryn M. Webb ◽  
Patricia M. Giltrap ◽  
Ian P. Adams ◽  
Jeff C. Peters ◽  
...  

2011 ◽  
Vol 5 (1) ◽  
pp. 135-141 ◽  
Author(s):  
Sébastien Terrat ◽  
Richard Christen ◽  
Samuel Dequiedt ◽  
Mélanie Lelièvre ◽  
Virginie Nowak ◽  
...  

2011 ◽  
Vol 86 (3) ◽  
pp. 397-400 ◽  
Author(s):  
Tom O. Delmont ◽  
Patrick Robe ◽  
Ian Clark ◽  
Pascal Simonet ◽  
Timothy M. Vogel

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