An overview of the Chloropidae (Diptera) of Saudi Arabia

Chloropidae are of major economic importance, since the larvae of some species are pests of cereals and grasses, some are parasitoids and predators, and adults of some Hippelates spp. visit and transmit or are suspected of transmitting yaws sores in man and animals and by feeding around the eyes are vectors of Brazilian Purpuric Fever. Within the framework of the exploration of the biodiversity of Diptera in Southwest Saudi Arabia a survey of the grass flies fauna in 18 sites in Jazan, Asir, and Najran in south-western Saudi Arabia was performed mainly using Malaise traps and sweep nets from 2010– to 2016. Sixty six species of 43 genera and three subfamilies of Chloropidae were identified and are recorded from Saudi Arabia, 20 of them for the first time and three are described as new species: Elachiptera arabica Deeming sp. n.; Kwarea ismayi Deeming sp.n. and Tricimba turneri Deeming sp.n.. This makes the total number of Chloropidae species in Saudi Arabia 95 (including 29 species previously recorded). Seventy images are presented. The species of Chloropidae listed are predominantly of Afrotropical/ Palaearctic origin. An updated checklist of Chloropidae species of Saudi Arabia is presented.This study adds new records of Chloropidae to the Saudi Arabian Diptera fauna, which will become reference points for research detailing the systematic geographic distribution and for identifying other specimens submitted for identification. Further species will undoubtedly be discovered with more research involving collecting and rearing methods.

Closed Complete Annotated Genome Sequences of Five Haemophilus influenzae Biogroup aegyptius Strains

Haemophilus influenzae biogroup aegyptius is a cause of conjunctivitis in children. Biogroup aegyptius strains also caused fatal outbreaks of invasive disease, known as Brazilian purpuric fever (BPF), in the 1980s. BPF is fatal if untreated. Here, we report the complete genome sequences of five strains of Haemophilus influenzae biogroup aegyptius.

Draft Whole-Genome Sequences of Haemophilus influenzae Biogroup aegyptius Strains Isolated from Five Brazilian Purpuric Fever Cases and One Conjunctivitis Case

Brazilian purpuric fever is a febrile hemorrhagic pediatric disease caused by Haemophilus influenzae biogroup aegyptius, a bacterium which was formerly associated with only self-limited purulent conjunctivitis. Here, we present draft genomes of strains from five Brazilian purpuric fever cases and one conjunctivitis case.

Emergence and Disappearance of a Virulent Clone of Haemophilus influenzae Biogroup aegyptius, Cause of Brazilian Purpuric Fever

SUMMARY In 1984, children presented to the emergency department of a hospital in the small town of Promissão, São Paulo State, Brazil, with an acute febrile illness that rapidly progressed to death. Local clinicians and public health officials recognized that these children had an unusual illness, which led to outbreak investigations conducted by Brazilian health officials in collaboration with the U.S. Centers for Disease Control and Prevention. The studies that followed are an excellent example of the coordinated and parallel studies that are used to investigate outbreaks of a new disease, which became known as Brazilian purpuric fever (BPF). In the first outbreak investigation, a case-control study confirmed an association between BPF and antecedent conjunctivitis but the etiology of the disease could not be determined. In a subsequent outbreak, children with BPF were found to have bacteremia caused by Haemophilus influenzae biogroup aegyptius (H. aegyptius), an organism previously known mainly to cause self-limited purulent conjunctivitis. Molecular characterization of blood and other isolates demonstrated the clonal nature of the H. aegyptius strains that caused BPF, which were genetically distant from the diverse strains that cause only conjunctivitis. This led to an intense effort to identify the factors causing the unusual invasiveness of the BPF clone, which has yet to definitively identify the virulence factor or factors involved. After a series of outbreaks and sporadic cases through 1993, no additional cases of BPF have been reported.

Cloning and Sequencing of a Genomic Island Found in the Brazilian Purpuric Fever Clone of Haemophilus influenzae Biogroup Aegyptius

ABSTRACT A genomic island was identified in the Haemophilus influenzae biogroup aegyptius Brazilian purpuric fever (BPF) strain F3031. This island, which was also found in other BPF isolates, could not be detected in non-BPF biogroup aegyptius strains or in nontypeable or typeable H. influenzae strains, with the exception of a region present in the type b Eagan strain. This 34,378-bp island is inserted, in reference to H. influenzae Rd KW20, within a choline transport gene and contains a mosaic structure of Mu-like prophage genes, several hypothetical genes, and genes potentially encoding an Erwinia carotovora carotovoricin Er-like bacteriocin. The product of the tail fiber ORF in the bacteriocin-like region shows a hybrid structure where the C terminus is similar to an H. influenzae phage HP1 tail protein implicating this open reading frame in altering host specificity for a putative bacteriocin. Significant synteny is seen in the entire genomic island with genomic regions from Salmonella enterica subsp. enterica serovar Typhi CT18, Photorhabdus luminescens subsp. laumondii TT01, Chromobacterium violaceum, and to a lesser extent Haemophilus ducreyi 35000HP. In a previous work, we isolated several BPF-specific DNA fragments through a genome subtraction procedure, and we have found that a majority of these fragments map to this locus. In addition, several subtracted fragments generated from an independent laboratory by using different but related strains also map to this island. These findings underscore the importance of this BPF-specific chromosomal region in explaining some of the genomic differences between highly invasive BPF strains and non-BPF isolates of biogroup aegyptius.