The purpose of my research is to explore the ways that intellectuals reinterpreted Eve using the Humanistic method during the Renaissance, questioning the naturalized relationship between women and sin. Humanism, a growing movement during the 15th century, placed emphasis on ascertaining meaning through analyzing works for their intended meaning and considering the context, while also revering God and antiquity alongside attention to the individual. Christine De Pizan and Isotta Nogarola use the Humanistic method of analysis in different ways to argue that Eve, and womankind, do not deserve a devious reputation and it is not justified by God. I will use historical artworks and writings to show how Eve was depicted prior to and during Renaissance Humanism. For example, Michelangelo’s Sistine Chapel depiction of the Fall fundamentally differs from other previous works by redistributing the culpability of Adam and Eve; no longer is Eve a sensual being, nor entirely to blame for the Fall. Additionally, as is necessary with any historical analysis, I will examine the contextual factors that allowed for the reinterpretation of Eve, especially by women, including the ways that both were a product and yet also ahead of their time. Whether the reinterpretations are pro-feminine or not is irrelevant in many respects, for the significance stems from women taking back a piece of historiography of the prototype woman, Eve.