Dynamics of Morphological Indicators of Blood of Piglets under the Influence of Iron Clathrochelate Complex and Cyanocobalamin

Iron deficiency anemia is one of the most common non-contagious diseases of piglets. Veterinary antianemic drugs have several drawbacks, so finding new medicines is an important current task for scientists. Therefore, the present study investigated the antianemic effect of iron (IV) clathrochelate in the organism of piglets. The subsequent studies included the exploration of its antianemic actions, particularly in combination with cyanocobalamin when this combination was administered to sows for prophylaxis in piglets. The experiment was carried out on 30 suckling piglets during the period of their detention with sows. According to the method of analogue groups, two groups of control (I) and experimental (II, each containing 15 animals) were formed and they were studied for 30 days. The piglets from five sows (three from each) were selected for the experimental group. During the pregnancy of these sows, 10 ml of 10% solution of iron (IV) IV clathrochelate and solution of cyanocobalamin were injected intramuscularly twice 7 and 14 days before their expected farrowing. For prevention of iron deficiency anemia, the traditional solution of iron dextran was administered once intramuscularly to piglets of the control group. The investigative material included the blood samples of piglets considering the dynamics of probable changes in the number of erythrocytes, hemoglobin content and hematocrit, and other morphological indicators and blood indices of piglets. The dynamics of changes in erythrocyte count, hemoglobin content, hematocrit, leukocytes and platelets, indices of blood almost did not differ from the dynamics of these values when using only 10% solution of iron (IV) clathrochelate for pregnant sows. The proposed scheme for the prophylaxis of iron deficiency anemia in piglets, involving simultaneous intramuscular injections of IV clathrochelate and cyanocobalamin to pregnant sows, is somewhat inferior to the previous preventive measures, which included only the intramuscular injections of iron (IV) IV clathrochelate, but it can be recommended as highly effective.

THE EFFECT OF N-ACETYLCYSTEINE AND DEXAMETHASONE PARENTERAL ADMINISTRATION ON THE BIOCHEMICAL PROCESSES IN THE AQUEOUS HUMOR OF THE ANTERIOR CHAMBER OF THE EYES IN RABBITS WITH EXPERIMENTAL IMMUNOGENIC UVEITIS

Objective. To evaluate the impact and compare the efficiency of parenteral administration of N-acetylcysteine (NAC) and dexamethasone when used as monotherapy or combination therapy in the treatment of experimental immunogenic uveitis (EIU) in rabbits, as well as to explore the changes of biochemical parameters in the aqueous humor of the eyes in experimental animals. Material and Methods. An experimental study was performed on 45 rabbits (90 eyes). Of these 5 healthy intact rabbits (10 eyes) served as a control group. Acute immunogenic uveitis was caused in 40 rabbits by injecting normal horse serum subcutaneously (5 ml) and then intravitreally (0.07 ml). The animals with experimental uveitis were divided into 8 groups (5 animals each). The first 4 groups – control-1, experiment-1, control-3, experiment-3 – received daily intramuscular injections of placebo, NAC, dexamethasone or a combination of NAC and dexamethasone respectively for 3 days, and thereafter they were withdrawn from the experiment. The remaining 4 groups – control-2, experiment-2, control-4, experiment-4 received, respectively, daily intramuscular injections of placebo, NAC, dexamethasone, a combination of NAC and dexamethasone for 7 days, and after that they were also withdrawn from the experiment. The drugs in the aforementioned groups were used from the moment of the horse serum intravitreal injection. When withdrawing animals from the experiment, aqueous humor was taken from the anterior chamber of their eyes, followed by the evaluation of protein (albumin) concentration and the number of leukocytes. Results. A significant elevation of albumin and the number of leukocytes in the aqueous humor of the eyes in the rabbits with experimental immunogenic uveitis was noted. NAC effectively reduced the level of albumin and the number of leukocytes in the aqueous humor. Dexamethasone showed more efficacy in reducing the investigated aqueous humor biochemichal parameters than NAC. Nevertheless, a synergism of the pharmacological action of NAC and dexamethasone was detected, since their combination had the greatest potency in reduction of albumin level and the number of leukocytes in the aqueous humor of the eyes in the rabbits with experimental immunogenic uveitis, even though the dosage of dexamethasone in the groups with combined (NAC and dexamethasone) therapy was reduced by 50% (1 mg / kg body weight). Conclusion. Parenteral administration of NAC significantly reduces inflammation in EIU. Combination of NAC and dexamethasone showed synergy of action in reducing the intensity of inflammatory process in rabbits with EIU, which is an objective rationale for including NAC in the complex therapy of uveitis, which in turn will reduce a single or course dose of dexamethasone and lower the risks of side effects caused by glucocorticoids.

335. Staphylococcus aureus Bacteremia as a Potential and Severe Complication from Intramuscular COVID-19 Vaccine Injection

Background Abscess formation and bacteremia following intramuscular injections are rare complications from vaccine injections, and they are most commonly seen in immunocompromised individuals. Staphylococcus aureus is one of the etiological agents that can be found during this complication. Spain started to vaccine its population at the beginning of 2021. We noticed an important increase in Staphylococcus aureus infections and bacteremia during this period of time, leading us to study the relationship with previous vaccination. Methods In this case series we present a cohort of twenty patients with Staphylococcus aureus bacteremia (SAB) during the study period (January 1, 2021 through May 31, 2021), attended in our Institution (Hospital Nuestra Señora de Sonsoles, Ávila, Spain). We tried to establish or at least create the debate of a possible relationship with a previous COVID-19 vaccine. Results From January 1, 2021 through May 31, 2021, 20 SAB were identified in our Institution. 13/20 patients were vaccinated (all of them with the mRNA vaccine type). 5/13 (38%) were male and 8/13 (62%) female. 10 of them (77%) received at least one dose of the vaccine before hospital admission, and 3 of them (23%) after admission. From the 10 previously COVID-19-vaccinated patients treated for SAB (CVPSAB), 4 died - 40% (2 deaths directly related to the SAB). Conclusion Although SAB may be a rare side effect after intramuscular injections or vaccines, it always implies an outstanding risk due to potential complications. Even if our study is not able to directly establish a link between SAB and previous vaccination, it implies a possible association between the vaccine injection and a threating disease (SAB). We should be aware of this probable relationship, so that we can maximize preventive measures. Disclosures All Authors: No reported disclosures

NICOLAU SYNDROME IN A CHILD FOLLOWING IV CLINDAMYCIN INJECTION-DECODING THE PURPLE PUZZLER

Nicolau syndrome is a rare complication of intramuscular injections caused by various drugs that present with intense pain and induration at the injection site. It is characterized by local aseptic skin necrosis and rarely, muscle necrosis on the injection site. It has rarely been reported to occur after intramuscular injections of anti-inammatory drugs, corticosteroids, local anesthetics, penicillin and interferon. Our literature review revealed no cases of Nicolau syndrome following intravenous (IV) clindamycin injections. Herein, we report a case of Nicolau syndrome that occurred after IV clindamycin injection in the left forearm of a 1.5-year child, that was notable because of the uniqueness of the syndrome coupled with the fact that it has not previously been reported after intravenous clindamycin injection.

The use of myosalvarsan in infants

Waldeyer (Derm. Ztschr. Bd. 56, H. 5, 29), given that it is often impossible for infants to use Neo preparations intravenously, tried intramuscular injections of myosalvarsan in the treatment of 27 syphilitic children. The dose, depending on the age, weight and constitution of the patient, was dissolved in minimal amounts of destil. water, and injections were made every 5-8 days of treatment; a total of 12-15 injections were made during the course. Three children out of 27 died (11.2%) and a. He sees the cause of death not in the drug, but in the weakness of children with congenital syphilis, who usually die from extraneous infections. Based on his observations, the author believes that in myosalvarsan we have a remedy that is not irritating and does not have side effects. The use is very simple. When properly dosed, it should be included in the anti-syphilitic arsenal in the treatment of lues in infants.

Experience of using intramuscular injections of ichthyol in acute and chronic diseases of the female genital area.

The effect of ichthyol, introduced psectually into the human body, has not yet been experimentally studied. In Russian and foreign literature, I have not found an answer to this question. Perhaps ichthyol plays the role of an activator of protoplasm, and perhaps the action of ichthyol fits into the theory of irritation of Beer. Sokolsky, Kenis and others consider the action of ichthyol as protein therapy.

Treatment of inflammatory diseases of the uterine appendages with turpentine. Fuchs (Zentr. T. Gyn., 1920, No. 4), Hinze (ib., No. 28) and Sonnenfeld (Berl. Klin. Woch., 1920, No. 30)

Treatment of inflammatory diseases of the uterine appendages with turpentine. Fuchs (Zentr. T. Gyn., 1920, No. 4), Hinze (ib., No. 28) and Sonnenfeld (Berl. Klin. Woch., 1920, No. 30) observed very good results from the treatment of salpingo-ophoritis with intramuscular injections of a mixture of turpentine with olive oil in a ratio of 1: 4.

Intravenous and combined methods of treatment of articular rheumatism

In recent years, we have used the following methods in the treatment of articular rheumatism: 1) intravenous infusion of the so-called Attritin 16% salicylic sodium solution + 4% coffeini n.- salic solution. 2) Intramuscular injections of milk in combination with intravenous infusions of Attritin'a. 3) Intravenous infusion of opsonized salicylic sodium and 4) Natr. salic, ex 10-12.0-200.0 per os - 4-6 tablespoons per day in combination with subcutaneous injections of autologous blood.