immunological synapses
Recently Published Documents


TOTAL DOCUMENTS

110
(FIVE YEARS 0)

H-INDEX

30
(FIVE YEARS 0)

2020 ◽  
Vol 32 (22) ◽  
pp. 2070167
Author(s):  
Sooseok Im ◽  
Donghyun Jang ◽  
Gurusamy Saravanakumar ◽  
Junseok Lee ◽  
Yeoul Kang ◽  
...  

2020 ◽  
Vol 32 (22) ◽  
pp. 2000020 ◽  
Author(s):  
Sooseok Im ◽  
Donghyun Jang ◽  
Gurusamy Saravanakumar ◽  
Junseok Lee ◽  
Yeoul Kang ◽  
...  

Cancers ◽  
2020 ◽  
Vol 12 (4) ◽  
pp. 806 ◽  
Author(s):  
Irina V Alekseenko ◽  
Igor P Chernov ◽  
Sergei V Kostrov ◽  
Eugene D Sverdlov

The failure of therapies directed at targets within cancer cells highlight the necessity for a paradigm change in cancer therapy. The attention of researchers has shifted towards the disruption of cancer cell interactions with the tumor microenvironment. A typical example of such a disruption is the immune checkpoint cancer therapy that disrupts interactions between the immune and the cancer cells. The interaction of cancer antigens with T cells occurs in the immunological synapses. This is characterized by several special features, i.e., the proximity of the immune cells and their target cells, strong intercellular adhesion, and secretion of signaling cytokines into the intercellular cleft. Earlier, we hypothesized that the cancer-associated fibroblasts interacting with cancer cells through a synapse-like adhesion might play an important role in cancer tumors. Studies of the interactions between cancer cells and cancer-associated fibroblasts showed that their clusterization on the membrane surface determined their strength and specificity. The hundreds of interacting pairs are involved in the binding that may indicate the formation of synapse-like structures. These interactions may be responsible for successful metastasis of cancer cells, and their identification and disruption may open new therapeutic possibilities.


Author(s):  
Anastasios Siokis ◽  
Philippe A. Robert ◽  
Philippos Demetriou ◽  
Audun Kvalvaag ◽  
Salvatore Valvo ◽  
...  

AbstractIntegrin and small immunoglobulin superfamily (sIGSF) adhesion complexes function physiologically in human immunological synapses (IS) wherein sIGSF complexes form a corolla of microdomains around an integrin ring and secretory core. The corolla recruits and retains the major costimulatory and checkpoint complexes that regulate the response to T cell receptor (TCR) engagement, making forces that govern corolla formation of particular interest. We developed a phenomenological agent-based model in order to test different hypotheses concerning the mechanisms underlying molecular reorganization during IS formation. The model showed that sIGSF complexes are passively excluded to the distal aspect of the IS as long as their interaction with the ramified F-actin transport network is absent or weaker than that of integrins. An attractive force between sIGSF adhesion and costimulatory/checkpoint complexes relocates the latter from the centre of the IS to the corolla. The simulations suggest that size based sorting interactions with large glycocalyx components as well as a short-range self-attraction between sIGSF complexes explain the corolla “petals”. These molecular and mechanistic features establish a general model that can recapitulate complex pattern formation processes observed in cell-bilayer and cell-cell interfaces.One Sentence SummaryComputer simulations of immunological synapses reveal the localization mechanisms of immunoglobulin superfamily adhesion and costimulatory/checkpoint complexes.


2019 ◽  
Vol 20 (18) ◽  
pp. 4509 ◽  
Author(s):  
Francisca Hofmann ◽  
Mariela Navarrete ◽  
Javiera Álvarez ◽  
Israel Guerrero ◽  
María Alejandra Gleisner ◽  
...  

Upon tumor antigen recognition, cytotoxic T lymphocytes (CTLs) and target cells form specialized supramolecular structures, called cytotoxic immunological synapses, which are required for polarized delivery of cytotoxic granules. In previous reports, we described the accumulation of connexin 43 (Cx43)-formed gap junctions (GJs) at natural killer (NK) cell–tumor cell cytotoxic immunological synapse. In this report, we demonstrate the functional role of Cx43-GJs at the cytotoxic immunological synapse established between CTLs and melanoma cells during cytotoxicity. Using confocal microscopy, we evaluated Cx43 polarization to the contact site between CTLs isolated from pMEL-1 mice and B16F10 melanoma cells. We knocked down Cx43 expression in B16F10 cells and evaluated its role in the formation of functional GJs and the cytotoxic activity of CTLs, by calcein transfer and granzyme B activity assays, respectively. We found that Cx43 localizes at CTL/B16F10 intercellular contact sites via an antigen-dependent process. We also found that pMEL-1 CTLs but not wild-type naïve CD8+ T cells established functional GJs with B16F10 cells. Interestingly, we observed that Cx43-GJs were required for an efficient granzyme B activity in target B16F10 cells. Using an HLA-A2-restricted/MART-1-specific CD8+ T-cell clone, we confirmed these observations in human cells. Our results suggest that Cx43-channels are relevant components of cytotoxic immunological synapses and potentiate CTL-mediated tumor cell killing.


2019 ◽  
Vol 5 (7) ◽  
pp. eaaw0315 ◽  
Author(s):  
Xingwang Zhao ◽  
Hengyi Xie ◽  
Meng Zhao ◽  
Asma Ahsan ◽  
Xinxin Li ◽  
...  

B cell activation is regulated by the stimulatory or inhibitory co-receptors of B cell receptors (BCRs). Here, we investigated the signaling mechanism of Fc receptor-like 1 (FcRL1), a newly identified BCR co-receptor. FcRL1 was passively recruited into B cell immunological synapses upon BCR engagement in the absence of FcRL1 cross-linking, suggesting that FcRL1 may intrinsically regulate B cell activation and function. BCR cross-linking alone led to the phosphorylation of the intracellular Y281ENV motif of FcRL1 to provide a docking site for c-Abl, an SH2 domain-containing kinase. The FcRL1 and c-Abl signaling module, in turn, potently augmented B cell activation and proliferation. FcRL1-deficient mice exhibited markedly impaired formation of extrafollicular plasmablasts and germinal centers, along with decreased antibody production upon antigen stimulation. These findings reveal a critical BCR signal-enhancing function of FcRL1 through its intrinsic recruitment to B cell immunological synapses and subsequent recruitment of c-Abl upon BCR cross-linking.


2019 ◽  
Vol 4 (33) ◽  
pp. eaav5445 ◽  
Author(s):  
Fella Tamzalit ◽  
Mitchell S. Wang ◽  
Weiyang Jin ◽  
Maria Tello-Lafoz ◽  
Vitaly Boyko ◽  
...  

Cytotoxic T lymphocytes (CTLs) kill by forming immunological synapses with target cells and secreting toxic proteases and the pore-forming protein perforin into the intercellular space. Immunological synapses are highly dynamic structures that boost perforin activity by applying mechanical force against the target cell. Here, we used high-resolution imaging and microfabrication to investigate how CTLs exert synaptic forces and coordinate their mechanical output with perforin secretion. Using micropatterned stimulatory substrates that enable synapse growth in three dimensions, we found that perforin release occurs at the base of actin-rich protrusions that extend from central and intermediate locations within the synapse. These protrusions, which depended on the cytoskeletal regulator WASP and the Arp2/3 actin nucleation complex, were required for synaptic force exertion and efficient killing. They also mediated physical deformation of the target cell surface during CTL–target cell interactions. Our results reveal the mechanical basis of cellular cytotoxicity and highlight the functional importance of dynamic, three-dimensional architecture in immune cell-cell interfaces.


2019 ◽  
Author(s):  
Moosung Lee ◽  
Young-Ho Lee ◽  
Jinyeop Song ◽  
Geon Kim ◽  
YoungJu Jo ◽  
...  

We propose and experimentally validate a label-free, volumetric, and automated assessment method of immunological synapse dynamics using a combinational approach of optical diffraction tomography and deep learning-based segmentation. The proposed approach enables automatic and quantitative spatiotemporal analyses of immunological synapse kinetics regarding morphological and biochemical parameters related to the total protein densities of immune cells, thus providing a new perspective for studies in immunology.


2018 ◽  
Author(s):  
Viveka Mayya ◽  
Edward Judokusumo ◽  
Enas Abu-Shah ◽  
Willie Neiswanger ◽  
Lance C Kam ◽  
...  

AbstractMemory T cells are endowed with multiple functional features that enable them to be more protective than naïve T cells against infectious threats. It is not known if memory cells have a higher synapse propensity, i.e. increased probability to form immature immunological synapses that then provide an entry into different modes of durable interaction with antigen presenting cells. Here we show that only human memory CD8 T cells have remarkably high synapse propensity compared to naïve counterparts. Such a dichotomy between naïve and memory cells is not observed within the human CD4 or murine CD8 T cell population. Increased surface expression of LFA1 contributes to the higher synapse propensity in human memory CD8 T cells. Finally, we show that higher synapse propensity in human memory CD8 T cells allows them to compete out naïve CD8 T cells from getting recruited to the response. This observation has implications for original antigenic sin and aging of the immune system in humans.


Sign in / Sign up

Export Citation Format

Share Document