Genetic differentiation of mainland-island sheep of Greece: Implications for identifying candidate genes for long-term local adaptation

In Greece, a number of local sheep breeds are raised in a wide range of ecological niches across the country. These breeds can be used for the identification of genetic variants that contribute to local adaptation. To this end, 50k genotypes of 90 local sheep from mainland Greece (Epirus, n = 35 and Peloponnesus, n = 55) were used, as well as 147 genotypes of sheep from insular Greece (Skyros, n = 21), Lemnos, n = 36 and Lesvos, n = 90). Principal components and phylogenetic analysis along with admixture and spatial point patterns analyses suggested genetic differentiation of ‘mainland-island’ populations. Genome scans for signatures of selection and genome-wide association analysis (GWAS) pointed to one highly differentiating marker on OAR4 (FST = 0.39, FLK = 21.93, FDR p-value = 0.10) that also displayed genome wide significance (FDR p-value = 0.002) during GWAS. A total number of 6 positional candidate genes (LOC106990429, ZNF804B, TEX47, STEAP4, SRI and ADAM22) were identified within 500 kb flanking regions around the significant marker. In addition, two QTLs related to fat tail deposition are reported in genomic regions 800 kb downstream the significant marker. Based on gene ontology analysis and literature evidence, the identified candidate genes possess biological functions relevant to local adaptation that worth further investigation.

Look Over the Horizon - Chicken Linkage Disequilibrium is Much More Complex Over Much Longer Distance than Previously Appreciated

BackgroundAppreciable Linkage Disequilibrium (LD) is commonly found between pairs of loci close to one another, decreasing rapidly with distance between the loci. This provides the basis studies to map Quantitative Trait Loci Regions (QTLRs), where it is custom to assume that the closest sites to a significant markers are the prime candidate to be the causative mutation. Nevertheless, Long-Range LD (LRLD) can also be found among well-separated sites. LD blocks are runs of genomic sites all having appreciable LD with one another. High LD and LRLD are often separated by genomic sites with which they have practically no LD. Thus, not only can LD be found among distant loci, but also its pattern may be complex, comprised of fragmented blocks. Here, chicken LRLD and LD blocks, and their relationship with previously described Marek’s Disease (MD) QTLRs, were studied in an F6 population from a full-sib advanced intercross line, and in eight commercial pure layer lines. Genome wide LRLD was studied in the F6 population by random samples of non-syntenic and syntenic marker pairs. To illustrate the relationship with QTLRs, LRLD and LD blocks in and between the MD QTLRs were studied by all possible marker pairs.Results LRLD was defined as r2 ≥ 0.7 over a distance ≥ 1 Mb, and 1.5% of all syntenic marker pairs were classified as LRLD. Complex fragmented and interdigitated LD blocks were found, ranging over distances from a few hundred to a few millions bases. Vast high, long-range, and complex LD was found between two of the MD QTLRs. Cross QTLRs STRING networks and gene interactions suggested possible origins of the exceptional LD between these two QTLRs.ConclusionsAll sites with high LD with a significant marker should be considered as candidate for the causative mutation, but, unlike the custom assumption, the causative mutation is not necessarily the one closest to the significant marker. Rather, the present results show that it can be located at a much larger distance from a significant marker than previously appreciated, beyond closer mutations. Thus, LRLD range and LD block complexity must be accounted for while interpreting genetic mapping studies.

Complex and long-range linkage disequilibrium and its relationship with QTL for Marek's Disease resistance in chicken populations

Chicken long-range linkage disequilibrium (LRLD) and LD blocks, and their relationship with previously described Mareks Disease (MD) quantitative trait loci regions (QTLRs), were studied in an F6 population from a full-sib advanced intercross line (FSAIL), and in eight commercial pure layer lines. Genome wide LRLD was studied in the F6 population by random samples of non-syntenic and syntenic marker pairs genotyped by Affymetrix HD 600K SNP array. To illustrate the relationship with QTLRs, LRLD and LD blocks in and between the MD QTLRs were studied by all possible marker pairs of all array markers in the QTLRs, using the same F6 QTLR genotypes and genotypes of the QTLR elements' markers in the eight lines used in the MD mapping study. LRLD was defined as r2 ≥ 0.7 over a distance ≥ 1 Mb, and 1.5% of all syntenic marker pairs were classified as LRLD. Complex fragmented and interdigitated LD blocks were found, over distances ranging from a few hundred to a few million bases. Vast high, long-range, and complex LD was found between two of the MD QTLRs. Cross QTLRs STRING networks and gene interactions suggested possible origins of this exceptional QTLRs' LD. Thus, causative mutations can be located at a much larger distance from a significant marker than previously appreciated. LRLD range and LD block complexity may be used to identify mapping errors, and should be accounted for while interpreting genetic mapping studies. All sites with high LD with a significant marker should be considered as candidate for the causative mutation.

Identification of Novel Marker–Trait Associations for Lint Yield Contributing Traits in Upland Cotton (Gossypium hirsutum L.) Using SSRs

Improving the yield of lint is the main objective for most of the cotton crop improvement programs throughout the world as it meets the demand of fiber for textile industries. In the current study, 96 genotypes of Gossypium hirsutum were used to find novel simple sequence repeat marker-based associations for lint yield contributing traits by linkage disequilibrium. Extensive phenotyping of 96 genotypes for various agronomic traits was done for two consecutive years (2018 and 2019) in early, normal, and late sown environments. Out of 168 SSR markers screened over the 96 genotypes, a total of 97 polymorphic markers containing 293 alleles were used for analysis. Three different models, i.e., mixed linear model (MLM), compressed mixed linear model (CMLM), and multiple locus mixed linear model (MLMM), were used to detect the significant marker–trait associations for six different environments separately. A total of 38 significant marker–trait associations that were common to at least two environments were considered as promising associations and detailed annotation of the significant markers has been carried out. Twenty-two marker–trait associations were found to be novel in the current study. These results will be very useful for crop improvement programs using marker-assisted cotton breeding.

Luminescence-Colour-Changing Sensing Toward Neurological Drug Carbamazepine in Water and Biofluids Based on a White-Light-Emitting CD/Ln-MOF/PVA Test Paper

Carbamazepine (CBZ) is a significant marker to reflect human health and anthropogenic pollution to the aqueous system. However, few CBZ chemo-sensors meeting allowable requirements are available in practice. For this...

Plurality in the Theological Struggle against Apartheid

The church struggle against apartheid remains a key case study in ecumenical public theology, with particular relevance for the Reformed tradition. The importance of Christian theology in both the justification of and opposition to apartheid is well known. Also, the process of ecumenical discernment for responding to apartheid became a significant marker in global ecumenical reflection on what today we might describe as public theology. However, the idea of a theological struggle against apartheid risks ironing out the different theological positions that oppose apartheid. This article highlights some of the attempts to analyze the theological plurality in responses to apartheid. Then it proceeds to present an alternative way of viewing this plurality by focusing on the way in which different classic theological questions were drawn upon to analyze apartheid theologically. Using as examples the important theologians David Bosch, Simon Maimela, and Albert Nolan, it highlights how apartheid was described as a problem of ecclesiology, theological anthropology, and soteriology. It argues that this plurality of theological analyses allows us to rediscover theological resources that might be of particular significance as race and racism take on new forms in either democratic South Africa or the contemporary world. Simultaneously, it serves as a valuable example in considering a variety of theological questions when theologically reflecting on issues of public concern.

The Conflict between Paganism and Christianity in Irish Theatre: A Cultural Study of O’Casey’s The Drums of Father Ned

While Irish theatre assures that it reflects Irish reality and aims at building a national identity in which religion is a significant marker, the plays of O’Casey contradict with this tendency. This study aims at discussing the conflict between an anti- Christian and pagan beliefs and Christian values in favor of anti- Christianity over Christianity. In this article, the researcher takes O'Casey’s The Drums of Father Ned as an example. The study discussed the tension between the pagan and Christian values as represented in the conflict between the pagan and religious characters, represented by Father Fillfogue who is the representative of Christianity; while The Celtic god Angus represents Pagan Celtic anti-Christian religion. Finally, the study proved that O'Casey presented a skeptical attitude toward Christianity and favored pagan Irish local, regional beliefs.