spontaneous differentiation
Recently Published Documents


TOTAL DOCUMENTS

86
(FIVE YEARS 2)

H-INDEX

23
(FIVE YEARS 0)

2021 ◽  
Author(s):  
Kavya Vipparthi ◽  
Kishore Hari ◽  
Priyanka Chakraborty ◽  
Subhashis Ghosh ◽  
Ankit Kumar Patel ◽  
...  

Author(s):  
Ai Yamada ◽  
Mariko Kinoshita ◽  
Sachiyo Kamimura ◽  
Kazuhiko Nakame ◽  
Hiroshi Moritake

2020 ◽  
Vol 15 (3) ◽  
pp. 646-661
Author(s):  
Francesco Aulicino ◽  
Elisa Pedone ◽  
Francesco Sottile ◽  
Frederic Lluis ◽  
Lucia Marucci ◽  
...  

2019 ◽  
Author(s):  
Francesco Aulicino ◽  
Francesco Sottile ◽  
Elisa Pedone ◽  
Frederic Lluis ◽  
Lucia Marucci ◽  
...  

AbstractThe Wnt/β-catenin signalling pathway is a key regulator of embryonic stem cell self-renewal and differentiation. Constitutive activation of this pathway has been shown to significantly increase mouse embryonic stem cell (mESC) self-renewal and pluripotency marker expression. In this study, we generated a novel β-catenin knock-out model in mESCs by using CRISPR/Cas9 technology to delete putatively functional N-terminally truncated isoforms observed in previous knock-out models. While we showed that aberrant N-terminally truncated isoforms are not functional in mESCS, we observed that canonical Wnt signalling is not active in mESCs, as β-catenin ablation does not alter mESC transcriptional profile in LIF-enriched culture conditions; on the other hand, Wnt signalling activation represses mESC spontaneous differentiation. We also showed that transcriptionally silent β-catenin (ΔC) isoforms can rescue β-catenin knock-out self-renewal defects in mESCs, cooperating with TCF1 and LEF1 in the inhibition of mESC spontaneous differentiation in a Gsk3 dependent manner.


2019 ◽  
Vol 234 (11) ◽  
pp. 20377-20391 ◽  
Author(s):  
Kengo Iwasaki ◽  
Motohiro Komaki ◽  
Keiko Akazawa ◽  
Mizuki Nagata ◽  
Naoki Yokoyama ◽  
...  

2019 ◽  
Vol 3 (4) ◽  
pp. 1800299 ◽  
Author(s):  
Alice C. Taylor ◽  
Citlali Helenes González ◽  
Patrizia Ferretti ◽  
Richard B. Jackman

2019 ◽  
Vol 35 (1) ◽  
pp. 114-124 ◽  
Author(s):  
F.J. Rodríguez-Lozano ◽  
M. Collado-González ◽  
C.J. Tomás-Catalá ◽  
D. García-Bernal ◽  
S. López ◽  
...  

2017 ◽  
Vol 8 (10) ◽  
pp. e3149-e3149 ◽  
Author(s):  
Ting Li ◽  
Hanzhi Zhao ◽  
Xu Han ◽  
Jiaying Yao ◽  
Lingling Zhang ◽  
...  

Sign in / Sign up

Export Citation Format

Share Document