alkaline proteinases
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2014 ◽  
Vol 10 (2) ◽  
pp. 295-304
Author(s):  
Bogusław Narzymski ◽  
Jadwiga Chmielnicka ◽  
Henryk Urbanek

Maximal proteolytic activity at pH 8 was noted in the culture filtrate from <i>Mucor microsorus</i> when the medium contained: 30 g maltose, 10 g pepsine peptone, 5 g CaCO<sub>3</sub>, 1 g KN<sub>2</sub>PO<sub>4</sub>, 0.5 g MgSO<sub>4</sub> 7H<sub>2</sub>O, 0.5 g KCL, 0.05 g FeSO<sub>4</sub>, 0.05 g ZnSO<sub>4</sub>, 0.05 g MnCl<sub>2</sub> in 1l.water.


2006 ◽  
Vol 61 (5-6) ◽  
pp. 445-452 ◽  
Author(s):  
Dessislava Georgieva ◽  
Nicolay Genov ◽  
Wolfgang Voelter ◽  
Christian Betzel

Catalytic efficiencies of proteinase K and mesentericopeptidase were determined using series of peptide-4-nitroanilide substrates and compared with those of subtilisin DY, savinase and esperase. For each enzyme the subsites S1-S4 were characterized. The data for the enzyme specificities were related to our high resolution X-ray models of the five enzymes and their complexes with peptides. The catalytic efficiencies of the alkaline proteinases are modulated by the hydrophobicity, solvent accessibility, flexibility and electrostatic effects in the substrate binding sites. The longer and nonpolar S1 loop offers more possibilities for hydrophobic interactions and increases the enzyme efficiency. S2 is a small narrow cleft which limits the possibilities for effective substitutions in P2. The wide specificity of S3 is due to its location on the protein surface of all investigated proteinases. The affinity of S4 for aromatic groups depends on the nature of the residues building the hydrophobic cavity.


2003 ◽  
Vol 37 (3) ◽  
pp. 349-356 ◽  
Author(s):  
Anja I. Pekkarinen ◽  
Tuija H. Sarlin ◽  
Arja T. Laitila ◽  
Auli I. Haikara ◽  
Berne L. Jones

1993 ◽  
Vol 115 (2) ◽  
pp. 158-160 ◽  
Author(s):  
S. I. Ogurtsov ◽  
A. S. Dukhanin ◽  
S. Yu. Mitroshina

1992 ◽  
Vol 114 (6) ◽  
pp. 1820-1822
Author(s):  
S. I. Ogurtsov ◽  
A. S. Dukhanin ◽  
S. Yu. Mitroshina

1991 ◽  
Vol 15 (1) ◽  
pp. 51-66 ◽  
Author(s):  
MIN-SOO HEU ◽  
JAE-HYEUNG PYEUN ◽  
HYEUNG-RAK KIM ◽  
J. SAMUEL GODBER

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