Electromagnetic Field Associated With Dermoscope Magnets May Affect the Safety of Cardiac Implanted Electronic Devices Patients

Dermoscopy is currently used as an auxiliary tool in general dermatology. Since some commercially available dermoscopes have built-in magnets, electromagnetic interference (EMI) may occur when examining cardiac implantable electronic devices (CIED) patients. The aim of the study was to create maps of electromagnetic fields defining a safe distance in terms of EMI. The study was performed in laboratory conditions using measuring equipment specially designed for this purpose. The following dermoscopes have been tested: Illuco IDS-1100, Visiomed Luminis, Visiomed Luminis 2, Heine NC2 with and without a contact plate, DermLite DL4, and DermLite Handyscope. Measurements were made for the following set of lift-off distances: 5, 10, 20, 30, 40, 50, and 150 mm. Each 2D scan consisted of 10-line scans shifted from each other by 10 mm. The strength of the magnetic field decreased with the distance from the faceplate. The distribution of the magnetic field differed depending on the position of the magnets. The highest magnetic field was recorded in the center of the Heine NC2 faceplate (up to 8 mT). In most cases, at a distance of 10 mm, the magnetic field strength was measured below 1 mT, with the exception of Heine NC2 and Heine NC2 with a contact plate. All tested dermoscopes generated a magnetic field of <1 mT at the distance of 20 mm. The use of dermoscopes with built-in magnets may affect the functioning of CIEDs, and the impact may vary depending on the type of dermoscope.

Analisis Sifat Dan Efektivitas Anti-Mikroba Minyak Atsiri Biji Pala (Myristica Fragrans) Untuk Pemurnian Kualitas Udara Pada Ruangan Iso Class 8

Minyak biji pala mengandung zat yang berfungsi sebagai anti-bakteri. Pemisahan minyak biji pala dengan metode soxhletasi. Minyak biji pala digunakan untuk meningkatkan kualitas udara ruangan dari segi penurunan jumlah bakteri dengan pengaplikasian pada diffuser. Hasil pengujian efektivitas minyak biji pala pada konsentrasi 0,1%; 0,3%; dan 0,5% untuk parameter Total Particle ukuran 0,5 �m terdapat peningkatan dari sebelum dilakukan pengujian rata-rata 993.923 partikel/m3 menjadi 2.329.135 partikel/m3, untuk partikel ukuran 5 �m dari rata-rata 3.110 partikel/m3 menjadi 4.965 partikel/m3. Parameter mikrobiologi seperti Settle Plate memberikan hasil penurunan jumlah bakteri dari konsentrasi 0% rata-rata 51 CFU/4 jam menjadi 23 CFU/4 jam pada konsentrasi 0,5%. Microbiological Active Air Sampling rata-rata 60 CFU/m3 pada konsentrasi 0% menjadi 23 CFU/m3 pada konsentrasi 0,5%. Contact Plate (dinding) rata-rata 2 CFU/plate pada konsentrasi 0% hingga 0 CFU/plate pada konsentrasi 0,5% dan pengujian Contact Plate (lantai) dari rata-rata 8 CFU/plate pada konsentrasi 0% hingga 5 CFU/plate pada konsentrasi 0,5%.

Frozen Cuttlefish (Sepia officinalis) Production Process with Contact Plate Freezing Method at PT. Karya Mina Putra, Rembang, Central Java

Cuttlefish is widely consumed by the public because it has a soft meat texture and high nutritional content. This makes the cuttlefish one of the mollusca that is a source of protein in addition to squid and octopus. The disadvantage of cuttlefish is that it is prone to deterioration in quality. One effort to prevent quality deterioration in cuttlefish is by freezing. The purpose of the implementation of this Field Work Practice is to decide the production process of frozen cuttlefish (Sepia officinalis) using the Contact Plate Freezing (CPF) method and to know the obstacles that occur in the production process. Field Work Practice was held in PT. Karya Mina Putra, Rembang, Central Java. The stages of the frozen cuttlefish production process at PT. Mina Putra's work includes the reception of raw materials, weighing I and sorting, labeling I, weighing II, washing, pan preparation, freezing, glazing, packaging and labeling II, storing in cold storage, and loading. The freezing process is carried out by the Contact Plate Freezing (CPF) method for 6 hours until the temperature reaches -40 ° C. The obstacle in the production process of frozen cuttlefish is there was a Contact Plate Freezing (CPF) machine damage and there are some employees who do not apply the SOP applied to prevent cross-contamination of the product.

Isolasi dan Karakterisasi Kapang Filoplan serta Serasah Daun di Lingkungan Laboratorium Biologi Universitas Diponegoro Dengan Metode Contact Plate

Fungi can be present on various substrates in the surrounding environment. The presence of these molds takes advantage of the nutrients that are attached to or contained in the substrate. The purpose of this study was to isolate and obtain various types of molds from various substrates. The method used in this study is a contact plate by attaching it to various objects or substrates, namely leaf litter, composter walls, tables, glass, and cabinets. The fungi obtained were 6 isolates, including those from the genus Aspergillus, Penicillium, Curvularia, and Alternaria,

Evaluation of Sampling Methods for Detection of Pathogens from Steel Surfaces; Contact Plates, Foam Swabs, and Flocked Swabs

Background: Contaminated healthcare surfaces can serve as reservoirs for the transmission of pathogens. Sensitive sampling methods are needed to investigate sources of pathogens for implementing effective disinfection strategies and thereby preventing environmental transmission. Conventional approaches employ swabs to sample environmental surfaces. Contact plates represent an alternative approach for sampling healthcare surfaces that does not require lab processing, though little is known about their performance. A contact plate is an agar plate that is overfilled with selective or nonselective media. It can be gently applied to the surface, then simply incubated at a temperature optimal for target organism (s), thus saving time and resources. Methods: In this study, contact plates containing trypticase soy agar with 5% sheep blood (TSAII), foam swabs, and flocked swabs were evaluated for their ability to recover 4 pathogens that persist on healthcare surfaces. Stainless-steel coupons (4 in2) were inoculated with the following pathogens (102 CFU): Acinetobacter baumannii (AB, strain type 12), carbapenemase-producing KPC+ Klebsiella pneumoniae (KP; ATCC BAA-1705); methicillin-resistant Staphylococcus aureus (MRSA; ATCC 43300); and vancomycin-resistant Enterococcus faecalis (VRE; Van A + 256). The plates were allowed to dry 1 hour. Sampling with CPs was performed in 2 ways; (1) a single contact plate was used to sample 1 stainless-steel surface and (2) a composite was collected by 3 sequential contact-plate samplings of the same stainless-steel surface. The contact plates were then incubated at 351C. Foam and flocked swabs were premoistened with phosphate-buffered saline + 0.02% polysorbate 80 (PBST) and were used to sample the stainless-steel coupons. Swabs were held for 1 hour and processed by sonication and vortexing in 5 mL of PBST, then the eluent was cultured and CFU counted. Mean percentage recoveries (%R) relative to the inoculum were calculated and compared. Results: When the %R for all 4 pathogens were pooled, the composite contact-plate sampling method yielded the highest, (P < .05) (66.0%; SD, 0.22), followed by the single contact plate method (39.7%; SD, 0.12), foam swab (32.9%; SD, 0.18), and flocked swab (20.3%; SD, 0.20). The composite contact plate method yielded the highest %R for VRE (102.1 %; SD, 0.17), and the lowest %R was observed when using flocked swabs to recover KP (6.3%; SD, 0.05). Conclusions: The contact-plate composite method may provide investigators with minimal environmental microbiology capacity an alternative method for environmental sampling and detection of organisms from surface areas (4 in 2) with low bioburden.Funding: NoneDisclosures: None

Study of contact plates recovery from pharmaceutical cleanroom surfaces across three-time ranges

Viable environmental monitoring methods remain primarily culture based. One example is with the contact plate. While the method is long-established, there remain aspects that are under-researched in relation to sampling. Factors affecting surface recovery relate to microbial adhesion, the type of surface, the sampling method and the time and pressure applied. This paper examines the effect of time, when a consistent pressure is applied, in relation to microbial recovery (for the organism Staphylococcus aureus) from two surfaces common to pharmaceutical facilities: stainless-steel and vinyl. The experimental results show that surface recovery was superior for vinyl compared with stainless-steel. For both surface types, a 20 second sampling time was shown to lead to a better recovery compared to a ten second sampling time (with a 30 second sampling time not leading to a significant improvement to the microbial surface recovery).