ENHANCING COGNITIVE FUNCTION OF HEALTHY WISTAR RATS WITH AQUEOUS EXTRACT OF Centella asiatica

Background: Centella asiatica (L.) Urb is a native herb from Asian countries such as India, China, and Indonesia. This herb has been widely used as a cure for various diseases. However, studies investigating the aqueous extract of Centella asiatica as a nootropic in healthy individuals are still very limited.Objective: This study aims to investigate the potential of aqueous extract of Centella asiatica in enhancing cognitive function of healthy male Wistar rats.Methods: Rats were randomly allocated to four treatment groups, i.e. without treatment and aqueous Centella asiatica extract at doses of 200, 400 and 800 mg/kg. To determine enhancement of cognitive function, novel object recognition (NOR) test was conducted after the course of treatment. Acetylcholine content was assessed by enzyme-linked immunosorbent assay.Results: There was a significantly high preference index towards the novel object in the NOR test in groups treated with 200 mg/kg and 800 mg/kg of the aqueous extract compared to control. This was further confirmed by a significant increase of brain acetylcholine content in rats treated with 200 mg/kg of the extract.Conclusion: Therefore, this study confirms that the aqueous extract is effective in enhancing cognitive performance of healthy Wistar rats.

ВИКОРИСТАННЯ НОВІТНІХ ІНФОРМАЦІЙНИХ ТЕХНОЛОГІЙ ДЛЯ АВТОМАТИЗАЦІЇ ВИЗНАЧЕННЯ ВМІСТУ АЦЕТИЛХОЛІНУ БІОЛОГІЧНИМ МЕТОДОМ

<p class="Barcode0">A new method of acetylcholine content in tissues measuring by biological method, allowing greatly decrease amount of factors that influence test accuracy, as well as time spent by automatization of greater part of test with using of new information technologies.</p>