Lactobacillus Spp. strains isolation, identification, preservation and quantitative determinations from the intestinal content and faeces of weaned piglets

The study aimed to isolate, identify, preserve and evaluate the quantitative level of the Lactobacillus strains from gut content and faeces of weaned piglets, 30-107 days old; to test the viability of these strains preserved at 4°C and room temperature. Lactobacillus strains were isolated, phenotypically identified and preserved from gut content and faeces of 20 weaned piglets. Identification was performed by morphological, cultural and biochemical character examination, using apiwebTM and ABIS online software. Lactobacillus spp. from intestinal content and faeces (106 – 109 CFU/g) and the viability of strains preserved at 4°C and at room temperature were also determined (from 38 days to 4 months). Twenty-six strains of L. acidophilus, L. fermentum, L. plantarum, L. salivarius and L. delbrueckii ssp. delbrueckii, from gut content and faeces of weaned piglets were isolated, phenotypic identified and preserved. Of these, L. fermentum, L. delbrueckii ssp. delbrueckii and L. acidophilus biotype 2 isolates were technologically and ecologically suitable for continuing the testing of probiotic traits.

Scrotal abscess caused by actinomycosis turicensis: about an observation and literature review

Actinomyces turicensis, Gram positive bacillus, immovable and anaerobic, is a saprophytic of the human natural cavities. The scrotal actinomycosis caused by Actinomyces turicensis is a rare location; it was described once in literature by a patient within gangrene infection. Reported here is a case of scrotal abscess and Actinomyces turicensis.The slow growth of the Actinomyces turicensis represents a diagnostic difficulty in which its utility is to extend the culture during five days. The identification based on biochemical character with the APICoryne is not always easy to succeed, in which the interest of the mass spectrometry and/or of the molecular biology.

Imaging of Big and Messy Biological Structures Using Electron Tomography

Any student of cell biology cannot help but be impressed with the exquisite organization of cellular and subcellular compartments, where Individual proteins and even nucleic acids are targeted to specific domains and held together in macromolecular assemblies to achieve orderly and efficient interactions. Perhaps nowhere is the specialization of cellular compartments more apparent than in the nervous system, where the cell contains multiple, well-defined functional domains, each with its own unique biochemical character.

A more detailed study of bile salt evolution, including techniques for small-scale identification and their application to amphibian biles

1. Methods have been developed for the isolation and identification of small amounts of bile salts and of bile acids and alcohols obtained by solvolysis. These methods involve preparative and analytical t.l.c., purification on columns of protonated Al2O3 and Sephadex LH-20 and also g.l.c.–mass spectroscopy of solvolysis products. 2. Application to 29 species of frogs and toads has confirmed the constancy of bile salt patterns in a single species, including colour phases in two instances, and has revealed great variations between different species in some genera (e.g. Rana, Ptychadena) and little difference between widely distributed species in others (e.g. Bufo). 3. Taxonomic deductions should be made with caution and with regard to the physiological significance of the biochemical character considered. The molecular differences found might be interpreted as indicating variations in the rate of evolution.