Teologie van die koninkryk (Basileiologie)? Teologies nagedink oor die plek en betekenis van die koninkryk van God in die teologie en die kerk

Hierdie artikel is ’n poging om aan te toon dat daar ’n groot leemte bestaan in die wyse waarop die klassieke leerstukke in dogmatiek in die verlede hanteer is, omdat dit ’n sentrale tema soos die koninkryk van God óf volkome geïgnoreer, óf totaal onderbelig het. Hierdie leemte word vervolgens nader ondersoek. Eers word aandag gegee aan die eskatologiese modelle wat in hierdie verband ontstaan het. Daarna word op die sentraliteit van die tema van die koninkryk in die Ou en Nuwe Testament gefokus en hierna word op die dinamiese uitwerking daarvan gelet wat dit op die samelewing behoort te hê. Die konklusie waartoe die outeur kom, is dat die tema van die koninkryk van God in die geheel gesien in die kerk en in die teologie totaal onderbeklemtoon was en steeds is. Vir ’n relevante kerkbeskouing (ekklesiologie) kan dit ’n groot bevryding bring indien die fokus van die kerk na die (gekome en komende) ryk van God verskuif. Die dogmatiek benodig ’n afsonderlike locus wat oor die Basileiologie handel.Theology of the kingdom (Basileiology)? Theological reflections on the place and role of the kingdom of God in church and theology. This article is an attempt to demonstrate that there is a great void in the way in which classical doctrine was treated in dogmatics in the past, since it either completely ignored a central theme like the kingdom of God, or shed altogether insufficient light on it. This void will subsequently be investigated more closely.Firstly, attention is given to the eschatological models which came into being for it. Next, the focus is on the centrality of the theme of the kingdom in the Old and New Testament after which its dynamic effect on society is pointed out.The conclusion reached by the author is that the theme of the kingdom of God was and is on the whole underemphasised in the church and in theology. For a relevant view of the church (ecclesiology) it could be liberating if the church shifted its focus to the kingdom of God (that has come and is coming). Dogmatics needs a separate locus dealing with Basileiology.

034 Independence of the Reduced Pigment (rp) Gene and the Y and Y2 Loci in Carrot (Daucus carota L.)

Five genes, including alleles at Y, Y1 and Y2, and the alleles I and IO, have been implicated in conditioning carrot root color. Various combinations of these alleles can condition white, yellow, and orange xylem and phloem color in carrot roots. The recently discovered reduced pigment gene, designated rp, conditions pale orange xylem and phloem and reduces carotenoid content by 92%. To determine if the rp gene is an allele of a previously described locus or a separate locus involved in pigment biosynthesis, we crossed rprp plants to plants with yellow phloem and yellow xylem (yellow/yellow) and to plants with purple phloem and yellow xylem (purple/yellow). We generated BC1 and F2 progenies for evaluation of segregation data. The expected genotype of plants with yellow xylem was yyY2Y2RPRP and the expected genotype of plants carrying rp was yyy2y2rprp. More than 1900 individual plants were evaluated for root color in 38 matings. In F2 progenies resulting from crosses of rprp × yellow/yellow, segregation data from 19 out of 20 families fit expected 12:3:1 (yellow: orange: rprp) or 15:1 (orange: non-orange) ratios (P < 0.001) indicating independence of the rp gene. In addition, 12 out of 16 families of F2 progenies from the cross between purple/yellow plants and rprp plants fit expected 12:3:1 (yellow: orange: rprp) ratios (P < 0.001) for a 2-gene model. These data support the hypothesis that the rp gene is independent and not linked to Y and Y2 genes.

The spliced leader sequence of Trypanosoma brucei has a potential role as a cap donor structure

Trypanosoma brucei brucei and other trypanosomatid species are unique among eucaryotes because transcription of their protein-coding genes is discontinuous. The 5' ends of their mRNAs consist of an identical 35-nucleotide spliced leader which is encoded at a separate locus from that for the body of the protein-coding transcript. We show here that the spliced leader transcript contains a 5' cap structure and suggest that at least one function of the spliced leader sequence is to provide a cap structure to trypanosome mRNAs.

The spliced leader sequence of Trypanosoma brucei has a potential role as a cap donor structure.

Trypanosoma brucei brucei and other trypanosomatid species are unique among eucaryotes because transcription of their protein-coding genes is discontinuous. The 5' ends of their mRNAs consist of an identical 35-nucleotide spliced leader which is encoded at a separate locus from that for the body of the protein-coding transcript. We show here that the spliced leader transcript contains a 5' cap structure and suggest that at least one function of the spliced leader sequence is to provide a cap structure to trypanosome mRNAs.

GENETIC ANALYSIS OF THE GENE FOR N-ACETYLGLUCOSAMINIDASE IN DICTYOSTELIUM DISCOIDEUM

ABSTRACT Three independent mutations affecting N-acetylglucosaminidase in Dictyostelium discoideum were mapped by the parasexual system and found to lie on linkage group IV. These mutations as well as two others were found to be recessive and noncomplementing in heterozygous diploids. Thus they all appear to affect the nagA locus. Since two of the mutations give rise to thermolabile enzyme, this defines the structural gene for N-acetylglucosaminidase. The enzyme is a homodimer of a 68,000 dalton subunit and thus would be expected to be determined by a single locus. The expression of this gene is regulated by the stages of development; however, it should be mentioned that none of the mutations fell in a separate locus that might determine a specific positive regulatory protein.

HIGH RESOLUTION RECOMBINATION ANALYSIS OF THE PYRIDOXINE-1 LOCUS OF NEUROSPORA

Seven alleles of the pyridoxine locus of Neurospora were mapped by inter-allelic crosses, using pyr-1 and col-4 as outside markers. Excluding a few exceptional crosses, the data give a linear order of the sites of the seven alleles within the gene, and confirm that allele 44204, previously assigned to a separate locus, pdx-2, is in fact an allele of pdx-1.