Variimorphobacter saccharofermentans gen. nov., sp. nov., a new member of the family Lachnospiraceae, isolated from a maize-fed biogas fermenter

Strain MD1T is an anaerobic, Gram-stain-negative bacterium isolated from a lab-scale biogas fermenter fed with maize silage. It has a rod-shaped morphology with peritrichously arranged appendages and forms long chains of cells and coccoid structures. The colonies of MD1T were white, circular, slightly convex and had a smooth rim. The isolate is mesophilic, displaying growth between 25 and 45 °C with an optimum at 40 °C. It grew at pH values of pH 6.7–8.2 (optimum, pH 7.1) and tolerated the addition of up to 1.5% (w/v) NaCl to the medium. The main cellular fatty acids of MD1T are C14:0 DMA and C16:0. Strain MD1T fermented xylose, arabinose, glucose, galactose, cellobiose, maltose, maltodextrin10, lactose starch, and xylan, producing mainly 2-propanol and acetic acid. The genome of the organism has a total length of 4163427 bp with a G+C content of 38.5 mol%. The two closest relatives to MD1T are Mobilitalea sibirica P3M-3T and Anaerotaenia torta FH052T with 96.44 or 95.8 % 16S rRNA gene sequence similarity and POCP values of 46.58 and 50.58%, respectively. As MD1T showed saccharolytic and xylanolytic properties, it may play an important role in the biogas fermentation process. Closely related variants of MD1T were also abundant in microbial communities involved in methanogenic fermentation. Based on morphological, phylogenetic and genomic data, the isolated strain can be considered as representing a novel genus in the family Lachnospiraceae , for which the name Variimorphobacter saccharofermentans gen. nov., sp. nov. (type strain MD1T=DSM 110715T=JCM 39125T) is proposed.

POTENTIAL BAGGASE AND MOLASSES WASTE SUGAR FACTORY FOR MIXED SUBSTRATES WITH COW MANURE IN FERMENTATION PROCESS METHANOGENIC

This study aims to analyze the potential of the sugar mill waste, namely baggase and molasses as a mixture of cow manure as a substrate in a methanogenic fermentation. This study uses 4 treatment that is P0 as controls (100% cow manure), later in other treatment using a mixture of cow manure comparison: baggase: molasses, P1 (50:20:30), P2 (50:30:20) and P3 ( 50: 40; 10). Parameters measured were nitrogen, carbon, ratio C / N and pH value. The analyze used in this research is statistic analysis. The results were obtained P3 which is a mixture of substrate (50:40:10) has an average value of the content of nitrogen, carbon, ratio C / N, and high pH value is compared to the other treatments. Ratio C / N at P3 of 24.47 closest Ratio C / N ideal for methanogenic fermentation which amounted to 25. The pH value of the treatment amounted to 7.05 billion P3 also meet the standards for process fermentation ranging from 6.6 to 7.6.