Bombyx mori pheromone-Binding Protein 1 (BmPBP1) in male moth antennae is a class of Odorant-Binding Proteins (OBPs), it can bind with the specific sex pheromone from female moth, thus initiates the males behaviors like seeking and mating, etc. It has been found that sex pheromone-binding protein 1 is differentially expressed in the antenna of male and female silkworm moths, however, the molecular mechanism of different PBP1 expression and its role in the information transmission are unclear. In this study, we successfully generated the BmPBP1 polyclonal antibody and used it to detect BmPBP1 expression in the silkworm moth antenna. Thus this work is helpful for further studies on the function of BmPBP1 in the information communication between male and female moths.