Degradation of Pyrazosulfuron Ethyl in Rice Ecosystem by Aspergillus terreus

Investigation on the degradation of pyrazosulfuron ethyl by Aspergillus terreus in the rice ecosystem was conducted at the Department of Biochemistry, UBKV, Pundibari Coochbehar, West Bengal, India. The soil was collected from the paddy field to isolate fungal inoculums. Results revealed that Aspergillus terreus can survive in minimal broth containing pyrazosulfuron ethyl at 1000 ppm and degrades through two major pathways, first involves the cleaves of sulfonylurea bridge resulting in the formation of two major metabolites viz., 2-amino-4, 6 –dimethoxyprimidine, 5-aminosulfonyl-1-methyl-pyrazole-4-carboxylic ethyl ester and, second was the cleavage of sulfonyl amide linkage which forms the metabolite viz; 1 methyl pyrazole-4-carboxylic acid ethyl ester,1 methyl pyrazole -4-carboxylic acid, 5-carbamoyl -1-methyl pyrazole -4-carboxylic acid ethyl-ester. The enzyme involved in these transformations can be utilized to decontaminate soil and water from Pyrazosulfuron ethyl residue. Even, the gene responsible for the production of these useful enzymes can be exploited for future research.

Triazole-Modified Nucleic Acids for the Application in Bioorganic and Medicinal Chemistry

This review covers studies which exploit triazole-modified nucleic acids in the range of chemistry and biology to medicine. The 1,2,3-triazole unit, which is obtained via click chemistry approach, shows valuable and unique properties. For example, it does not occur in nature, constitutes an additional pharmacophore with attractive properties being resistant to hydrolysis and other reactions at physiological pH, exhibits biological activity (i.e., antibacterial, antitumor, and antiviral), and can be considered as a rigid mimetic of amide linkage. Herein, it is presented a whole area of useful artificial compounds, from the clickable monomers and dimers to modified oligonucleotides, in the field of nucleic acids sciences. Such modifications of internucleotide linkages are designed to increase the hybridization binding affinity toward native DNA or RNA, to enhance resistance to nucleases, and to improve ability to penetrate cell membranes. The insertion of an artificial backbone is used for understanding effects of chemically modified oligonucleotides, and their potential usefulness in therapeutic applications. We describe the state-of-the-art knowledge on their implications for synthetic genes and other large modified DNA and RNA constructs including non-coding RNAs.

Oxidative Peptide Bond Formation of Glycine-Amino Acid using 2-(Aminomethyl)malononitrile as a Glycine Unit

Amide linkage of glycine-amino acid was synthesized by coupling of substituted 2-(aminomethyl)malononitrile as a C-terminal glycine unit and N-terminal amine using CsOAc and O2 in aqueous solution. This is a...

Green Solvents for the Formation of Amide Linkage

Amide bond is among the most commonly performed transformation within the pharmaceutical industry and the wider chemical industry. Current methods towards its installation in organic compounds frequently relies on the...

Transition-Metal-Catalyzed Intermolecular C–H Carbonylation toward Amides

The amide linkage is one of the most important structural moieties in both chemistry and biology. Here, we briefly discuss recent advances in catalytic intermolecular C–H carbonylation reactions for the synthesis of amides, with particular attention to our intermolecular C–H amidation of arenes with carbon monoxide and organic azides to produce amides.1 Introduction2 Representative Methods for Amide Synthesis3 C–H Aminocarbonylation with Carbon Monoxide and Amines4 C–H Amidation to Amides with Carbon Monoxide and Azides5 Summary and Outlook