Bioaccessibility of Polycyclic Aromatic Hydrocarbons (PAHs) in Grilled Meat: The Effects of Meat Doneness and Fat Content

Exposure to polycyclic aromatic hydrocarbons (PAHs) through diet is gaining concern due to the risk it poses to human health. This study evaluated the bioaccessibility of PAHs contained in charcoal-grilled beef and chicken in different segments of the gastrointestinal tract (GIT) with regard to the degree of doneness and fat content of the meats. The levels of 15 PAHs in the grilled meat samples and bioaccessible fractions were determined using high-performance liquid chromatography (HPLC) equipped with PAH column, and UV and fluorescence detectors. Total PAHs were found in beef (30.73 ng/g) and chicken (70.93 ng/g) before its digestion, and different PAHs’ bioaccessibility were observed in the different segments of GIT, with the highest in the stomach followed by the small intestine, despite the relatively higher bioaccessibility of individual PAHs in grilled beef as compared to those in grilled chicken. Additionally, the PAHs’ bioaccessibility increased with the increase in the degree of doneness. Positive linear correlation was observed for the PAHs’ bioaccessibility and the fat contents of grilled meat. Overall, this study highlights the influence of meat doneness (cooking time) and fat contents on the bioaccessibility and bioaccumulation of PAHs.

The Effects of Three Traditional Smoking Methods on the Concentrations of Polycyclic Aromatic Hydrocarbons (PAHS) in Smoked Fishes

The effects of three traditional smoking methods on the concentrations of Polycyclic Aromatic Hydrocarbons (PAHs) in smoked fishes were studied to determine the concentration of PAHs in locally available and commonly consumed smoked fish species. Samples of two highly traded species of fish, Scomber scombrus and Horse markerel, among the low income people for immediate consumption were purchased from the market and processed using sawdust smoke, firewood smoke and charcoal smoke respectively. Some of the fresh fishes were also analyzed as control. The PAHs content were extracted with standard dichloromethane using solid-liquid extraction, and analyzed using Gas chromatography – Mass spectrophotometer (GC-MS) method. The results showed that fish samples processed with sawdust smoke recorded the highest concentrations of total PAHs, having 1.295 mg/kg in Horse markerel and 2.020 mg/kg in Scomber scombrus, followed by firewood smoked samples with total PAHs content of 0.910mg/kg in Horse markrel and 1.175 g/kg in Scomber scombrus while charcoal smoked samples recorded the least total PAHs levels of 0.590 mg/kg in Horse markerel and 0.960 mg/kg in Scomber scombrus. Benzo(a)pyrene concentrations which is usually used to estimate the carcinogenicity of other PAHs was below detection level in both species of fish. PAH4 was proposed by European food safety authority, recommendation level of 30 mg/kg was concluded by the EU regulation. Any PAHs have been associated with intense carcinogenicity in humans, and thus have implication for the quality and safety of these fish products. Therefore, it is imperative that regulatory bodies conduct awareness campaigns to educate the smoked fish processors, traders and consumers on the need to discourage the use of sawdust in smoking fish and adopt safer and improved methods of smoking fishes.

Pollution investigation and risk assessment of polycyclic aromatic hydrocarbons in soil and water from selected dumpsite locations in rivers and Bayelsa State, Nigeria

The transfer ratio of polycyclic aromatic hydrocarbons (PAHs) from soil dumpsite to borehole water is dependent of polluting source and exposure matrices that causes immerse health risk to man and environment over a period of time. PAHs were assessed in selected soil dumpsite and borehole water located at Rivers state (Eleme, Eliozu, Eneka, Oyigbo, and Woji) and Bayelsa state (Yenagoa), Nigeria. Soil samples were collected at four different points 30 m (North, South, East and West) locations at a depth of 15 cm for each dumpsite using soil auger while control samples were collected 200 m away (farmland), where there were little anthropogenic activities and no presence of active dumpsites. Borehole water samples were collected from 300 m distance, which were packaged in an amber container, labeled, and transported to the laboratory for analysis. Standard analytical methods were employed. PAHs concentrations were analyzed using gas chromatography- mass spectrometry (GC-MS) after extraction of water and soil using liquid-liquid and soxhlet extraction methods respectively and clean-up of the extracts, thereafter the laboratory data generated were subjected to statistical analysis. Total PAHs (ΣPAHS) concentrations in soil samples from the study sites ranged from 2.4294 mg/kg in Yenagoa to 5.1662 mg/kg in Eleme while in water samples the total PAHs ranged from 1.3935 mg/L in Woji to 3.009 mg/L in Eleme. The total PAH concentrations in the soil were above the Agency for Toxic Substances and Disease Registry levels of 1.0 mg/kg for a considerably contaminated site except for the control sites. The total concentration of carcinogenic PAHs ranged from 0.0038 to 1.1301mg/kg in soil samples and 0.0014 to 0.9429 mg/L in borehole water samples, therefore raising concern of human exposure via food chain. The results indicate that low molecular weight PAHs were more dominant than high molecular weight PAHs in both soil and water samples, however molecular diagnostic ratio shows that pyrogenic activities are major sources of PAHs as compared to petrogenic origin. Multivariate analysis (principal component analysis and Pearson correlation) showed strong negative correlation implying that they were from dissimilar sources and different migratory route. Cancer and non-cancer risk showed that children were more at risk compared to adults, where inhalation exposure were major contribution as compared to ingestion and dermal exposure, as such there is a need to implement regulatory laws on indiscriminate release of PAHs contaminants to maintain sustainability.

Measurement of polycyclic aromatic hydrocarbons (PAHs) in edible mushrooms (raw, grilled and fried) using MSPE-GC/MS method: a risk assessment study

The purpose of this study was to evaluate the concentration of PAHs and health risk in edible mushrooms using magnetic solid phase extraction (MSPE) and gas chromatography/mass spectrometry (GC/MS) method. Based on the results, the concentration of total PAHs was in the range from 0.82 to 6.25 µg/kg (with the means of 3.85 ± 0.24 µg/kg). In this study, acenaphthylene (Ace) had the maximum mean level in mushroom samples (0.84 ± 0.19 μg/kg) and benzo(a)pyrene (BaP), acenaphthene (Ac), phenanthrene (Pa), dibenzo[a,h]anthracene (DahA), benzo(a)anthracene (BaA), fluoranthene (Fl), indeno[1,2,3-cd]pyrene (IP) and benzo[g,h,i]perylene (BgP) had minimum mean level (non-detected). The highest mean values of total PAHs (4.88 ± 0.30 μg/kg) was detected in grilled mushroom samples and lowest mean of total PAHs (2.77 ± 0.04 μg/kg) was detected in raw mushroom samples. The concentration of contamination in edible mushrooms was raw < fried > grilled, respectively. Furthermore, heat map visualization was applied to evaluate the relationship between the quantity and type of 16 PAHs in edible mushroom samples. Ultimately, the incremental lifetime cancer risk in edible mushrooms for adults and children was 6.85E-08 and 3.47E-07, respectively, which was lower than the acceptable risk limit (10–6) and all of the edible mushrooms sold in Tehran were considered safe for consumers.

Predicting the Total PAHs Concentrations in Sediments from Selected Congeners Using a Multiple Linear Relationship

In this study, four congeners, i.e., naphthalene (Nap), acenaphthylene (Acy), phenanthrene (Phe) and benz(a)anthracene (BaA), screened out by hierarchical clustering analysis from 16 PAHs, are the characteristic PAHs congeners in emission sources and in sediments. Novel multiple linear relationships, i.e., the total PAHs emission factors and the total PAHs concentration with the emission factors of Nap, Acy, Phe and BaA and their sediment concentrations data reported in the past 30 years were established, respectively. The same characteristic congeners observed for PAHs in sediments and in emission sources indicates that the concentration of PAHs in sediments are largely dependant on the PAHs emission. Moreover, the ratio of multicomponents coefficient from the multiple linear relationship in sediments to that in emission sources correlated positively with the logKow values and biodegradation half-lifes of four characteristic congeners, indicating PAHs concentrations in sediments are also alternated by their environmental behaviors including sorption and biodegradation. However, the established relationship gives a useful way to predicted C∑PAHs in sediments without the PAHs emissions data and can eliminate the effects of environmental behaviors and the difference of emissions in various regions.

INCIDENCES OF POLYCYCLIC AROMATIC HYDROCARBONS IN ROASTED PLANTAINS OBTAINED WITHIN OSOGBO METROPOLIS, NIGERIA

The present study was conducted to assess the carcinogenic and non-carcinogenic health risks of PAHs contamination in roasted plantain collected from three different locations within Osogbo metropolis, Nigeria. The PAHs were extracted using Soxhlet extraction technique with n-hexane and dichloromethane (3:1) as the extracting solvent. A column, packed with silica gel, was used for clean-up process and the levels of the targeted PAHs were determined by a gas chromatograph–flame ionization detector (GC-FID). The result revealed that the concentrations of total PAHs detected in the roasted samples at location A, B, C were 3.958 μg/kg, 3.654 μg/kg and 3.217 μg/kg. The HMW carcinogenic PAHs constitute about 32% of the total PAHs in the roasted plantain samples where benzo(a)pyrene, which is a marker for carcinogenicity, was found to be 9%. Comparing these results with the regulatory limits, all the analyzed roasted plantain samples were found below the maximum residual limit (MRL) of 5 μg/kg, but the calculated carcinogenic risk (CR) presented value greater than 1×10-4, signifying health risk associated with the consumption of the roasted plantain. The major source of the PAHs using diagnostic ratios (Fla/Pyr and Ph/Ant) showed that the charcoal used in roasting is a major contributing factor to the high levels of PAHs detected in the roasted plantain samples.

Characterization for Types and Percents of Polycyclic Aromatic Hydrocarbons in Sera of Workers at Fuels Stations at Baghdad

Allpolycyclic aromatic hydrocarbons (PAHs) have a hightoxicity, and/or carcinogenic and \ormutagenic to most microorganisms andhumans.Main aim to this study is to providecontemporary information (quantitative and qualitative)PAHs in sera of fuel stations, workers whom exposure with petroleum products.The study includes 60 male petrol-filling workers in 5 petrol stations around Baghdad. Theirages were between (20 – 50) years. For compared the results a 30 sera samples from healthyindividuals was collected with corresponding age-matched as control group. The samples werecollected from October 2017 until April 2018. The BMI was calculated for all subjects.Technique of High performance liquid chromatography (HPLC) was used to detect thepresence of PAHs quantitatively and qualitatively in the sera of all subjects.The mean level of total PAHs in sera of workers was 1275.65± 934.267 higher than that forhealthy subjects 67.380 ± 46.16 with significant value p=0.033.Also the types and percent ofPAHs in the sera of workers were differ.For studying the epidemiologic,no correlation betweenthe percent of % PAHs in sera of workers who exposure to petroleum withoutaffected withtheirage and BMI.

Speculative Carcinogenicity Assessment of Polycyclic Aromatic Hydrocarbons Found in Some Anti-Diarrheal Herbal Drugs Sold in Ondo State of Nigeria

The conviction that herbal drugs have enormous health benefits has led to increase the rate of their consumption by Nigerians. The aim of this study was to assess the carcinogenic property of some popularly consumed anti-diarrheal herbal drugs via polycyclic aromatic hydrocarbons (PAHs) quantification. Three prevalent anti-diarrhea herbal drugs , Odunmo herbal drug (Hibiscus rosa-sinensis and Bacopamonnieri), Orogun herbal mixture (Hibiscus sabdariffaI and Hedera helix), and Alora herbal syrup (Aloe vera and Hibiscus sabdariffaI) were bought for the purpose of this study and they were coded as samples A, B, and C, respectively. The ultrasonic extraction of the herbal drugs was carried out using standard procedures. The crude extracts obtained were purified using a chromatographic method. The concentrations of PAHs were quantified using gas chromatography flame ionization detector (GCFID). The diagnostic indices, group distribution, toxicity equivalence and exposure dosage were estimated. The cancer risk values were theoretically speculated based on concentrations of PAHs in the tested herbal drugs, associated with the published estimates of each concentration to cause cancer and the calculated exposure doses of the anti-diarrhea herbal drug samples were within three age groups (children, Preteen and adult). The highest concentration of total PAHs was observed in sample A (58.2815mg/kg) and the lowest concentration in sample B (44.1898mg/kg), but the concentration of total PAHs in sample C was 47.4169 mg/kg. The highest percentage of carcinogenic PAHs in the anti-diarrheal herbal drugs was found in sample C (48.66%) and the lowest in sample B (38.17%). The diagnostic indices confirmed a pyrogenic source of PAHs. Group distribution of PAHs showed that the herbal drugs are weakly carcinogenic due to high concentrations of low and moderate molecular weight PAHs. The cancer risk estimated for all the age groups where below the limit established by the United State Environmental Protection Agency (USEPA) for cancer (1 x 10-6). This ascertained that the use of these herbal drugs cannot cause cancer. However, consumers of these herbal drugs should take necessary precautions as excessive intake can lead to dangerous health implications.

Pah Content of Clarais gariepinus Harvested from Ekulu River, Eastern Nigeria Contaminated with Effluents Generated from a Roofing Sheet Industry Risk Impact Assessment

Aim: This study evaluated PAH content and health risks associated with consuming Clarais gariepinus (cat fish) from Ekulu Rivers, in Enugu, Nigeria fed with roofing sheet company effluent. Place and Duration of Study Design: Ekulu River, the largest body of water in the city of Enugu in Enugu State, south-eastern Nigeria, is a 25-kilometer long river (16mi) and it originates in the same city as well. The city is located on latitude 06o 21o N and 06o 30o and longitude 07o 26o E and 07o 37 Eo. Methodology: This analysis was conducted with the use of Gas Chromatography – Mass Spectroscopy (GC-MS) machine equipped with Flame ionization detector (FID). The health risks were evaluated by the mathematical models stipulated by USEPA and WHO. Results: The PAHs identified from the Clarias gariepinus samples were Acenaphthene, Acenaphthylene, Naphthalene, Fluorene, Phenanthrene, Anthracene, Flouranthene, Pyrene, Benzo(α)pyrene and Benzo (g-h-i)perylene. Benzo(α)pyrene and Benzo (g-h-i)perylene were predominant. The quantity of PAHs detected in all fish samples including the control ranged from below detectable limit (BDL) through 0.001 to 0.0786mg/kg. The LMW PAHs detected were 60 % while the HMW PAHs were 40 % of the total PAHs in isolated. The total PAH concentration observed from the different point locations were 0.1003 mg/kg, 0.0977 mg/kg, 0.1102 mg/kg and 0.0414 mg/kg for the downstream, upstream, POD and control respectively. The HQ and HI obtained in all point locations were < 1. The ILCR of all the PAHs detected were in the range of 10-5 to 10-9. The benzo (α) pyrene in cat fish obtained from the POD has the most carcinogenic potency and also recorded the maximum limit (5E -03 mg/kg). Conclusion: it is pertinent to enlighten the fish consumers and mongers on the dangers posed by the consumption of fish from Ekulu River and also caution industries with injudicious effluent disposal into water bodies.

ANALYSIS OF PRODUCE WATER FROM EBEDEI FLOW PLANT STATION, DELTA STATE

Produce water from Ebedei flow station (Delta State) at three points was analysed. Discharged point (A), midstream (B) and downstream (C) were a mile apart. The metal concentration of iron, zinc, cadmium, chromium, nickel, lead and manganese were carried out using standard methods. Lead levels at discharged point and down stream were higher than World Health Organization (WHO) standard limits. The polynuclear Aromatic hydrocarbon were determined using high performance liquid chromatography (HPLC). The total PAHs of sample (A) was 3.63ppm, (B) 52.05 and (C) 59.50 ppm respectively. The pH were within WHO limits of 8.10 and 8.20, conductivity in μs/cm was 20.10 and28.10 within acceptable range. Chloride was between 8, 130.00ppm and 22, 131.53 ppm higher than WHO and effluent discharge limits, while turbidity was between 46ppm-60ppm which was also higher. Hence there is need for holistic monitoring and remediation of produce water in the environment.