mitochondrial dna stability
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eLife ◽  
2018 ◽  
Vol 7 ◽  
Author(s):  
Dorine Jeanne Mariëtte du Mee ◽  
Maxim Ivanov ◽  
Joseph Paul Parker ◽  
Stephen Buratowski ◽  
Sebastian Marquardt

Most DNA in the genomes of higher organisms does not code for proteins. RNA Polymerase II (Pol II) transcribes non-coding DNA into long non-coding RNAs (lncRNAs), but biological roles of lncRNA are unclear. We find that mutations in the yeast lncRNA CUT60 result in poor growth. Defective termination of CUT60 transcription causes read-through transcription across the ATP16 gene promoter. Read-through transcription localizes chromatin signatures associated with Pol II elongation to the ATP16 promoter. The act of Pol II elongation across this promoter represses functional ATP16 expression by a Transcriptional Interference (TI) mechanism. Atp16p function in the mitochondrial ATP-synthase complex promotes mitochondrial DNA stability. ATP16 repression by TI through inefficient termination of CUT60 therefore triggers mitochondrial genome loss. Our results expand the functional and mechanistic implications of non-coding DNA in eukaryotes by highlighting termination of nuclear lncRNA transcription as mechanism to stabilize an organellar genome.


Oncogenesis ◽  
2017 ◽  
Vol 6 (7) ◽  
pp. e362-e362 ◽  
Author(s):  
C Tang ◽  
Y Hou ◽  
H Wang ◽  
K Wang ◽  
H Xiang ◽  
...  

2016 ◽  
Vol 37 (6) ◽  
Author(s):  
Srujan Kumar Matta ◽  
Gautam Pareek ◽  
Kondalarao Bankapalli ◽  
Anjaneya Oblesha ◽  
Patrick D'Silva

ABSTRACT Mitochondrial life cycle and protein import are intricate cellular processes, which require precise coordination between the transport machineries of outer and inner mitochondrial membranes. Presequence translocase performs the indispensable function of translocating preproteins having N-terminal targeting sequences across the inner membrane. Tim23 forms the core of the voltage-gated import channel, while Tim17 is presumed to maintain the stoichiometry of the translocase. However, mechanistic insights into how Tim17 coordinates these regulatory events within the complex remained elusive. We demonstrate that Tim17 harbors conserved G/AXXXG/A motifs within its transmembrane regions and plays an imperative role in the translocase assembly through interaction with Tim23. Tandem motifs are highly essential, as most of the amino acid substitutions lead to nonviability due to the complete destabilization of the TIM23 channel. Importantly, Tim17 transmembrane regions regulate the dynamic assembly of translocase to form either the TIM23 (PAM)-complex or TIM23 (SORT)-complex by recruiting the presequence translocase-associated motor (PAM) machinery or Tim21, respectively. To a greater significance, tim17 mutants displayed mitochondrial DNA (mtDNA) instability, membrane potential loss, and defective import, resulting in organellar dysfunction. We conclude that the integrity of Tim17 transmembrane regions is critical for mitochondrial function and protein turnover.


2014 ◽  
Vol 1837 ◽  
pp. e24
Author(s):  
Luis Alberto Luévano Martínez ◽  
Maria Fernanda Forni ◽  
Valquiria Tiago dos Santos ◽  
Nadja C. Souza-Pinto ◽  
Alicia J. Kowaltowski

2010 ◽  
Vol 24 (7) ◽  
pp. 2334-2346 ◽  
Author(s):  
Maria D. Aamann ◽  
Martin M. Sorensen ◽  
Christina Hvitby ◽  
Brian R. Berquist ◽  
Meltem Muftuoglu ◽  
...  

PLoS ONE ◽  
2009 ◽  
Vol 4 (11) ◽  
pp. e7902 ◽  
Author(s):  
María Cerezo ◽  
Hans-Jürgen Bandelt ◽  
Idoia Martín-Guerrero ◽  
Maite Ardanaz ◽  
Ana Vega ◽  
...  

2003 ◽  
Vol 2 (4) ◽  
pp. 809-820 ◽  
Author(s):  
Micheline K. Strand ◽  
Gregory R. Stuart ◽  
Matthew J. Longley ◽  
Maria A. Graziewicz ◽  
Olivia C. Dominick ◽  
...  

ABSTRACT In a search for nuclear genes that affect mutagenesis of mitochondrial DNA in Saccharomyces cerevisiae, an ATP-NAD (NADH) kinase, encoded by POS5, that functions exclusively in mitochondria was identified. The POS5 gene product was overproduced in Escherichia coli and purified without a mitochondrial targeting sequence. A direct biochemical assay demonstrated that the POS5 gene product utilizes ATP to phosphorylate both NADH and NAD+, with a twofold preference for NADH. Disruption of POS5 increased minus-one frameshift mutations in mitochondrial DNA 50-fold, as measured by the arg8m reversion assay, with no increase in nuclear mutations. Also, a dramatic increase in petite colony formation and slow growth on glycerol or limited glucose were observed. POS5 was previously described as a gene required for resistance to hydrogen peroxide. Consistent with a role in the mitochondrial response to oxidative stress, a pos5 deletion exhibited a 28-fold increase in oxidative damage to mitochondrial proteins and hypersensitivity to exogenous copper. Furthermore, disruption of POS5 induced mitochondrial biogenesis as a response to mitochondrial dysfunction. Thus, the POS5 NADH kinase is required for mitochondrial DNA stability with a critical role in detoxification of reactive oxygen species. These results predict a role for NADH kinase in human mitochondrial diseases.


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