A novel hyperthermophilic methylglyoxal synthase: molecular dynamic analysis on the regional fluctuations

Two putative methylglyoxal synthases, which catalyze the conversion of dihydroxyacetone phosphate to methylglyoxal, from Oceanithermus profundus DSM 14,977 and Clostridium difficile 630 have been characterized for activity and thermal stability. The enzyme from O. profundus was found to be hyperthermophilic, with the optimum activity at 80 °C and the residual activity up to 59% after incubation of 15 min at 95 °C, whereas the enzyme from C. difficile was mesophilic with the optimum activity at 40 °C and the residual activity less than 50% after the incubation at 55 °C or higher temperatures for 15 min. The structural analysis of the enzymes with molecular dynamics simulation indicated that the hyperthermophilic methylglyoxal synthase has a rigid protein structure with a lower overall root-mean-square-deviation value compared with the mesophilic or thermophilic counterparts. In addition, the simulation results identified distinct regions with high fluctuations throughout those of the mesophilic or thermophilic counterparts via root-mean-square-fluctuation analysis. Specific molecular interactions focusing on the hydrogen bonds and salt bridges in the distinct regions were analyzed in terms of interatomic distances and positions of the individual residues with respect to the secondary structures of the enzyme. Key interactions including specific salt bridges and hydrogen bonds between a rigid beta-sheet core and surrounding alpha helices were found to contribute to the stabilisation of the hyperthermophilic enzyme by reducing the regional fluctuations in the protein structure. The structural information and analysis approach in this study can be further exploited for the engineering and industrial application of the enzyme.

‘Tales of Symphonia ’: extinction dynamics in response to past climate change in Madagascan rainforests

Madagascar's rainforests are among the most biodiverse in the world. Understanding the population dynamics of important species within these forests in response to past climatic variability provides valuable insight into current and future species composition. Here, we use a population-level approach to analyse palaeoecological records over the last 5300 years to understand how populations of Symphonia cf. verrucosa became locally extinct in some rainforest fragments along the southeast coast of Madagascar in response to rapid climate change, yet persisted in others. Our results indicate that regional (climate) variability contributed to synchronous decline of S . cf. verrucosa populations in these forests. Superimposed on regional fluctuations were local processes that could have contributed or mitigated extinction. Specifically, in the forest with low soil nutrients, population model predictions indicated that there was coexistence between S . cf. verrucosa and Erica spp., but in the nutrient-rich forest, interspecific effects between Symphonia and Erica spp. may have pushed Symphonia to extinction at the peak of climatic change. We also demonstrate that Symphonia is a good indicator of a threshold event, exhibiting erratic fluctuations prior to and long after the critical climatic point has passed.

The Cordilleran Ice Sheet: One Hundred and Fifty Years of Exploration and Discovery

ABSTRACT Present concepts about the Cordilleran Ice Sheet are the product of observations and ideas of several generations of earth scientists. The limits of glaciation in the Cordillera were established in the last half of the nineteenth century by explorers and naturalists, notably G. M. Dawson, R. G. McConnell, and T. C. Chamberlin. By the turn of the century, the gross configuration of the Cordilleran Ice Sheet had been determined, but the causes of glaciation and ice-sheet dynamics remained poorly understood. This early period of exploration and discovery was followed by a transitional period, from about 1900 to 1950, during which a variety of glacial landforms and deposits were explained (e.g., Channeled Scablands of Washington; "white silts" of southern British Columbia), and conceptual models of the growth and decay of the ice sheet were proposed. Shortly after World War II, there was a dramatic increase in research into all aspects of glaciation in the Canadian Cordillera which has continued unabated to the present. Part of the research effort during this period has been directed at resolving the Cordilleran Ice Sheet in both time and space. Local and regional fluctuations of the ice sheet have been reconstructed through stratigraphie and sedimentological studies, supported by radiocarbon and other dating techniques. Compilations of late Pleistocene ice-flow directions have shown that the Cordilleran Ice Sheet was a mass of coalescent glaciers flowing in a complex fashion from many montane source areas. During the postwar period, research has also begun or advanced significantly in several other disciplines, notably glaciology, process sedimen-tology, geomorphology, paleoecology, and marine geology. Attempts are now being made to quantitatively model the Cordilleran Ice Sheet using computers and the geological database assembled by past generations of earth scientists.

The Convergence of the Regional Twinning Rates in Sweden, 1751–1960

Sweden has, as a whole nation, the oldest continuous population register of births, including twin and higher multiple maternities, starting in the 17th century. Until the 1920s, the rates of multiple maternities in Sweden were among the highest known among Europeans. Strong secular and regional fluctuations were noted. Some of the eastern counties showed especially marked decreasing trends. The twinning rate had no clear associations with the anthropometric and serological data. In this paper we study the temporal and regional variations of the twinning rate in the 25 counties of Sweden from 1751 to 1960. Different statistical methods were applied in order to test the hypothesis that, irrespective of the initial levels, the twinning rates for the counties converge towards a common low level. We present and interpret a geometrical model for the trends of the regional twinning rates. We also analyze the regional heterogeneity using the ranges and the coefficients of variation of the regional twinning rates. All the methods gave consistent results, supporting our convergence hypothesis that the regional differences in the twinning rate are gradually disappearing. In addition, this study supports our earlier findings that the regional heterogeneity cannot be explained by differences in the distribution of maternal age and parity. We suggest that the convergence may be caused by increased urbanization and industrialization and by the increased interregional migration of citizens as a consequence of better communications, which lead to the breaking up of isolates and decreased endogamy.