Fingerprinting molecular and isotopic biosignatures on different hydrothermal scenarios of Iceland, an acidic and sulfur-rich Mars analog

Detecting signs of potential extant/extinct life on Mars is challenging because the presence of organics on that planet is expected to be very low and most likely linked to radiation-protected refugia and/or preservative strategies (e.g., organo-mineral complexes). With scarcity of organics, accounting for biomineralization and potential relationships between biomarkers, mineralogy, and geochemistry is key in the search for extraterrestrial life. Here we explored microbial fingerprints and their associated mineralogy in Icelandic hydrothermal systems analog to Mars (i.e., high sulfur content, or amorphous silica), to identify potentially habitable locations on that planet. The mineralogical assemblage of four hydrothermal substrates (hot springs biofilms, mud pots, and steaming and inactive fumaroles) was analyzed concerning the distribution of biomarkers. Molecular and isotopic composition of lipids revealed quantitative and compositional differences apparently impacted by surface geothermal alteration and environmental factors. pH and water showed an influence (i.e., greatest biomass in circumneutral settings with highest supply and turnover of water), whereas temperature conditioned the mineralogy that supported specific microbial metabolisms related with sulfur. Raman spectra suggested the possible coexistence of abiotic and biomediated sources of minerals (i.e., sulfur or hematite). These findings may help to interpret future Raman or GC–MS signals in forthcoming Martian missions.

Hydrothermal eruption dynamics reflecting vertical variations in host rock geology and geothermal alteration, Champagne Pool, Wai-o-tapu, New Zealand

Hydrothermal eruptions are characterised by violent explosions ejecting steam, water, mud, and rock. They pose a risk to tourism and the operation of power plants in geothermal areas around the world. Large events with a severe destructive threat are often intensified by the injection of magmatic fluids along faults and fractures within volcano-tectonic rifting environments, such as the Taupo Volcanic Zone. How these hydrothermal eruptions progress, how craters form and the scale of ejecta impacts, are all influenced by the local geology and reservoir hydrology. By analysing breccia lithology, undisturbed strata proximal to the explosion sites, and conducting tailored decompression experiments, we elucidate the eruption sequence that formed Champagne Pool, Wai-o-tapu, New Zealand. This iconic touristic site was formed by a violent hydrothermal eruption at ~ 700 years B.P. Samples from undisturbed drill cores and blocks ejected in the eruption were fragmented in shock-tube experiments under the moderate pressure/temperature conditions estimated for this system (3–4 MPa, 210–220 °C). Our results show that this was a two-phase eruption. It started with an initial narrow jetting of deep-sourced lithologies, ejecting fragments from at least a 110-m depth. This event was overtaken by a larger, broader, and dominantly shallower eruption driven by decompression of much more geothermal fluid within a soft and porous ignimbrite horizon. The second phase was triggered once the initial, deeper-sourced eruption broke through a strong silicified aquitard cap. The soft ignimbrite collapsed during the second-phase eruption into the crater, to repeatedly choke the explosions causing short-term pressure rises that triggered ongoing deeper-sourced eruptions. The eruption spread laterally also by exploiting a local fault. These results are relevant for hydrothermal eruption hazard scenarios in environments where strong vertical variations in rock strength and porosity occur.

Petrographic and spectral study of hydrothermal mineralization in drill core from Hawaii: A potential analog to alteration in the martian subsurface

Continuous rock core was collected for 1764 m (5786’) on the Pohakuloa Army Training base near the center of the big island of Hawaii. The core traverses basaltic lava flows from the volcano's shield-building phase, and perched aquifers and higher temperature groundwaters were encountered. The collected samples record water-rock interactions of basaltic materials in a setting that may be a model for groundwater interactions on Mars. We collected visible and infrared point spectra of materials in the lowest portion of the core, where alteration was noted to become more prominent. We identified three types of phyllosilicate spectral signatures and three types of zeolites. The phyllosilicates show similarity to those identified on Mars using data from the Compact Reconnaissance Imaging Spectrometer for Mars (CRISM). Based on the field survey, 25 depths were selected for sampling and petrographic analysis of thin sections. The spectral data and thin section work have a strong agreement in the types of materials identified by the two different techniques. Both the spectral and petrographic data indicate low to moderate temperature geothermal alteration occurred in the lower half of the core. The field spectra are a useful reconnaissance tool for selecting mineralogic diversity for subsequent higher resolution and more time-consuming laboratory analysis.

Morpho-anatomical and physiological traits of Agrostis castellana living in an active geothermal alteration field

Agrostis castellana is one of the few plants colonizing one of the most extreme geothermal alteration fields characterized by low pH and high temperature of soil.The study of species surviving in these multi-stress habitats can help to know more in deep the adaptive ability of plants. In this work morpho-anatomical, functional and physiological traits of leaves of A. castellana living few meters from fumaroles were studied, focusing on their putative ecological significance.Some typical xeromorphic traits occurred in the leaves of these plants: abundant cutinization, cell wall thickening, slightly convolute and adaxially ribbed leaf blades, tissutal reinforcements by sclereids, pubescence, protected stomata and densely packed mesophyll. Abundant pectins, important in both water balance adjusting and as monosaccharide source, were observed in epidermal cell walls. Despite the low value of relative water content, indicative of a disturbed hydric state, oxidative damage, in terms of lipid peroxidation, indicative of membrane damage, was significantly lower than in other plants of the same environment, probably tanks to an adequate antioxidant response based mainly on enzymatic machinery. Interestingly catalase activity was not inhibited by extreme conditions of the geothermal alteration field as in other plants of the same habitat. In conclusion, a cooperation of xeromorphic traits and antioxidant response seems to make A. castellana able to survive in such a prohibitive environment.