Confocal Raman microspectral analysis and imaging of the drug response of osteosarcoma to cisplatin

Confocal Raman microspectral analysis and imaging was used to elucidate the drug response of osteosarcoma (OS) to cisplatin. Raman spectral data were obtained from OS cells that were untreated (UT...

Application of a multispectral assay for evaluation of the morphofunctional status of immunocompetent cells in cattle with retroviral diseases

Microspectral analysis is a highly informative way to study the morphofunctional status of cells, including immunocompetent ones. Purpose of the study was an application it for studding of bovine lymphocytes in cattle with enzootic leukemia and viral immunodeficiency, which is important for diagnostics and differential diagnostics of diseases, as well as for analyzing of animal homeostasis and making a prognosis of the disease. Leukemia and immunodeficiency are classified as hematopathological processes. Therefore, we used the hematological method as a comparative method. Spectral analysis of stained with a Leukodif 200 kit agranulocytes was carried out using the universal color analyzer microscope-spectrophotometer LOMO MSFU-K. The value of the light absorption intensity was measured in the visible-light spectrum from 300 to 700 nm. The results of microspectral analysis showed that for cows’ lymphocytes with combined pathology (BLV/BIV), the absorption values in the spectrum of eosin U and azure II were 351.2 ± 17.6 and 751.4 ± 37.6 counts, respectively. For animals with BLV and BIV mono-infection, these indicators were 253.3 ± 12.7; 383.3 ± 19.2 and 371.5 ± 18.5; 500.2 ± 24.9 counts. While in intact cows, these indicators were registered at the level of 210.3 ± 10.5 and 173.6 ± 8.6 counts. In intact animals, the acidic and basic components ratio in the cell was uniformly proportional, the ratio coefficient was 0.83 ± 0.04. For BIV-, BLV- and BLV/BIV-infected animals, this coefficient was 1.34 ± 0.06, 1.51 ± 0.08 and 2.13 ± 0.11, that was in 1.6, 1.8 and 2.6 times higher than in intact animals, which might be an indicator of metabolic acidosis in the cell. The scientific novelty of the research is that the signs of hypoxia and inflammation detected by hematological analysis correlate with the results of microspectral analysis and indicate a change in the homeostasis of the infected animal’s organism, including at the cell level.

Microspectral laser analysis of selected dental implants

Aim. In the paper the microspectral analysis of selected dental implants was presented.Material and methods. The following implants were analysed: Osteoplant, Keystone Dental, Mozo Grau, Alpha Bio, Sky, and Implant Direct. The probes of each implant were analysed in the laser spectrophotometer.Results. The result of the study was: Keystone Dental, Mozo Grau, Alpha Bio and Implant Direct titanium implants were shown to include aluminium and vanadium components while the Osteoplant and Sky implants to be made of pure titanium.