Identification of allelic combinations of the Ppd-D1, Vrn-A1, Vrn-B1 and Vrn-D1 genes in common wheat lines obtained in the National Center of Grain named after P. P. Lukyanenko

An analysis of the allelic composition of the genes determining photoperiodic sensitivity (Ppd-D1) and the need for vernalization (Vrn-A1, Vrn-B1, Vrn-D1) was carried out in 286 common wheat lines obtained in the National Center of Grain named after P. P. Lukyanenko with the use of allele-specific primers. The analyzed samples were distributed over 21 haplotypes; the dominant allele of the Ppd-D1a gene prevailed in the studied material. 123 lines of common wheat carry a combination of D-RRD alleles. The lines that can be attributed to the group of alternate wheat (R-RDR, R-RRD) were identified. All studied samples carry the recessive allele of at least one VRN1 gene.

Photoperiodic sensitivity and Ppd-1 genotypes of bread wheat alternative varieties

Aim. Study the reaction to photoperiod and identify Ppd-1 genotypes of modern alternative bread wheat varieties of various geographical origins. Methods. Plants growing under reduced and prolonged days, hybridological analysis of photoperiodic sensitivity of semi-diallelic F2 hybrids, calculations of average values, their errors and c2, multiplex STS-PCR with specific primers to the Ppd-D1 gene. Results. The phenotypic and genotype differences were detected, Ppd-1 genotypes of 10 alternative bread wheat varieties of different geographical origins were found. Conclusions. Afina, Palada, Solomia, Shestopalivka, Yara, Demir 2000, L897J23 varieties slowly or average react to reduce of day duration because of gene Ppd-D1a presence in their genotypes. Lastivka, Khutorianka, Zumoiarka varieties are highly photoperiodic sensitive genotypes and carriers of Ppd-1 genes recessive alleles only. From this view point, these three varieties correspond to the criteria of "typical alternative varieties". Keywords: wheat, alternative varieties, photoperiodic sensitivity, DNA markers, gene, Ppd-1.

DROUGHT RESISTANCE AND PRODUCTIVITY OF WHEAT AND SOYBEAN ISOGENIC LINES WITH DIFFERENT PHOTOPERIODIC SENSITIVITY

The results of the study of drought tolerance of isogenic (NILs – near isogenic lines) by genes PPD (photoperiod) wheat lines and isogenic by genes ЕЕ (early maturation) soybean lines, that control the photoperiodic sensitivity are presented. In field experiments the photoperiodic sensitivity of the lines when grown under natural long days (16 hours at a latitude of Kharkov) and under artificial short-day (9 hours) is determined. The results showed that line PРD-D1A and PPD-A1a wheat and soybean lines L 71-920 had a weak photoperiodic sensitivity (weak PPDS) and line PPD-B1a wheat and soybean lines L 71-920 - strong photoperiodic sensitivity (strong PPDS). Wheat and soybean lines with weak PPDS were more productive. When simulating drought action on seed germination (20% strength mannitol solution - rapid method), it was showed that the seeds of soybean and wheat lines with weak PPDS have a higher germination than seeds of the lines with strong PPDS. When simulating soil drought (30% FMC – field moisture capacity of the soil) under growing experiment, it was revealed that the biomass accumulation indices of plants, leaf relative water content (RWC) and proline content in leaves lines with weak PPDS were higher than in the photoperiodic lines with strong PPDS. So, all used methods for determining drought tolerance showed that the low photoperiodic sensitivity lines are more resistant to drought. It is assumed that wheat genes PPD and soybean genes EE can participate in the formation of resistance to drought. Genotypes with low photoperiodic sensitivity should be used in breeding soybean and wheat drought resistance.

TRIBE TRITICEAE L. AND THE BIOCENOTIC MECHANISMS OF ADAPTABILITY

<p>The cultural species in the process ontogenesis and phylogenesis to favorable and unfavorable environmental conditions produce a number of biological mechanisms (molecular genetic, physiological, biochemical, morphological, biocenotic), plants identification behind them reflects the degree of their ecological of adaptability, plasticity and stability. Studying the and comparison of morphological parameters and relations with consort-species and representatives of tribe <em>Triticeae</em> allowed to find out what plants differently realize their life potential. Which are based on structural and functional features biocenotic mechanisms that manifested in adaptive properties. On example cultural cereal species shows that the basic mechanisms of adaptability are: mechanisms of functional sustainability, morphological tolerance and ontogenetic avoidance. The first group is associated with functional parameters forming and manifestation life potential of plants (accumulation protein, accumulation gluten, duration assimilatory ability flag leaf; erection leaves the upper tier; depth of node tillering; strength of the stem, ie the, low penchant to lodging; total tillering plants; synchronicity growth of main stem; the intensity fading ear after full ripeness.</p><p>The second group includes mechanisms of morphological tolerance (hairiness of leaves, stems; wax-colored bloom; plaza of leaf; type of bush; density head; beardedness; glossy coating of leaf, culm; glaucous color of leaf, culm; placing spicate of scales near granule; plant height). To mechanisms of ontogenetic avoidance relating such as mismatch of pathogen, phytophage and plant; photoperiodic sensitivity; duration interphase periods in particular florification, ripening; duration of vegetation period; duration of primary dormancy (latent period); multivariation of synontоgenesis; photoperiodic sensitivity.</p><p>Knowing the biocenotic mechanisms formation of adaptability cultural species discloses up new opportunities in clarifying the fundamental bases of adaptation and is of practical importance in the management of vitality and seed productivity.</p>