Laboratory Evaluation of a Neem Product Against DBM Larvae, 1995

Treatments were evaluated using the leaf dip method. Head cabbage was seeded in community pots. Each pot containing approximately 10 cabbage plants in the 5 true leaf seedling stage was inverted and dipped in a test insecticide mix for about 30 sees for complete coverage. The dipped plants were allowed to air dry. For each dip, 1 liter insecticide mix was prepared based on field rate concentrations of 100 gal/acre. Leaves from treated plants were detached and placed in a ventilated plastic petri dish. DBM larvae from a laboratory colony that originated from individuals collected from a cabbage field at Kula, Hawaii and Kamuela, Hawaii were used. Ten late 2nd instars were placed on each leaf. Fresh leaves from the original treated plant were added every two days. The number of dead larvae was counted at 24-hour intervals. Larvae were recorded as dead when there was no movement when probed.

Laboratory Evaluation of Two IGR Insecticides Against DBM Larvae, 1995

Treatments were evaluated using the leaf dip method. Head cabbage was seeded in community pots. Each pot containing approximately 10 cabbage plants in the 5-7 true leaf seedling stage was inverted and dipped in a solution of the test materials for about 30 sees for complete coverage. The dipped plants were allowed to air dry. For each dip, 1 liter solution of the test material was prepared based on field rate concentrations of 100 gal/acre. Leaves from treated plants were detached and placed in a ventilated plastic petri dish. DBM larvae from a laboratory colony that originated from individuals collected from a cabbage field at Kula, Hawaii and Kamuela, Hawaii were used. Ten 2nd instars were placed on each leaf. Each treatment was replicated 10 times. Xentari was used as the treated check. The number of dead larvae was counted at 24-hour intervals. Larvae were recorded as dead when there was no movement when probed. Leaves were changed every other day with fresh leaves from the previously treated pots.

Laboratory Evaluations of Bioinsecticides Against Dbm Larvae, 1994

Treatments were evaluated using the leaf dip method. Head cabbage was seeded in community pots. Each pot contained approximately 10 cabbage plants. Potted head cabbage in the 5 true leaf seedling stage were dipped in each respective solution for complete coverage and allowed to air dry. Leaves from treated plants were detached and placed in a ventilated plastic petri dish. DBM larvae from a laboratory colony that originated from individuals collected from a cabbage field at Kula, Hawaii were used. Ten newly hatched larvae were placed on each leaf. Fresh leaves from the original treated plant were added every two days. The number of dead larvae was counted at 24 or 48 hour intervals. Larvae were recorded as dead when there was no movement when probed.

Improved methods for using glass coverslips in cell culture and electron microscopy.

For many applications, cells or tissue must be cultured on an optical surface of high quality. For such applications laboratories often prepare "special dishes," which are made by affixing a glass coverslip beneath a hole in a plastic petri dish bottom. In this report, we offer an improved method, using Parafilm as a dry mount adhesive, for the preparation of special dishes, and show that the resulting dish is non-toxic to neurons in culture. The Parafilm bond is stable at 60 degrees C, permitting electron microscopy resins to be poured directly into the dishes and cured. The glass coverslip can be readily removed from the cured resin mechanically. The techniques we describe offer time-saving and reliable improvements for the use of glass coverslips in cell culture and electron microscopy.