MODIFIKASI METODE ISOLASI DNA CETYL TRIMETHYLAMMONIUM BROMIDE (CTAB) UNTUK SAMPEL EPITEL PIPI MANUSIA

Isolation of deoxyribonucleotide (DNA) is an important step in molecular analysis. In this process, DNA must be obtained in sufficient quantities and in good quality for any further analysis. The Cetyl Trimethylammonium Bromide (CTAB) method is commonly used in DNA isolation of plant or fungal. This method is an alternative in DNA isolation since it is easy and inexpensive. This study aims to modify the CTAB method for DNA isolation from human cheek epithelium for any molecular analysis. Epithelial cells were taken from the oral cavity of the researcher. The isolation protocol included cell lysis step with CTAB buffer and proteinase-K, purification step with the addition of chloroform:isoamylalcohol (24:1), precipitation step with isopropanol. The results of the ratio analysis of DNA spectrophotometer at wavelengths of 260 and 280 nm in the range of 1.73-1.85. The quality of DNA isolation was observed by agarose gel electrophoresis and a firm band was obtained after Ethidium Bromide staining. The DNA concentration in both methods ranged from 400-480 mg/mL. The time required for both methods ranges from 2.5-3 hours. The modified CTAB method DNA isolation protocol produces DNA that has good quality and quantity for molecular analysis processes, such as Polymerase Chain Reaction (PCR).

On the origin of controlled anisotropic growth of monodisperse gold nanobipyramids

We elucidate the crucial role of the cetyl trimethylammonium bromide (CTAB) surfactant in the anisotropic growth mechanism of gold nano-bipyramids, nano-objects with remarkable optical properties and high tunability.

Development of an SSR-based DNA fingerprinting method for black wattle (Acacia mearnsii De Wild)

Background: The most commonly used method for extracting DNA from plant leaf tissue involves cetyl trimethylammonium bromide but some species, such as Acacia mearnsii, contain high levels of secondary metabolites and polysaccharides that interfere with this process. Various modifications have been proposed for effective removal of these biomolecules but these methods can be time consuming. Therefore, this study was initiated to optimise the cetyl-trimethylammonium bromide protocol for the extraction of high-quality genomic DNA and to develop a fingerprinting tool using cross species transferable simple sequence repeat markers for genetic diversity studies in A. mearnsii. Methods: Five CTAB-based modification were examined and 49 cross-species microsatellite markers, developed for several Acacia species, were tested in four multiplex panels of A. mearnsii populations. Results: The modified protocol yields high quantity and quality DNA from A. mearnsii leaves using high concentration of NaCl to remove polysaccharides and polyvinylpolypyrrolidone (PVPP) to eliminate polyphenols during DNA purification. In addition, omitting the selective precipitation and NaCl gradient steps in the extraction protocol, enabled us to extract DNA 10–20 min faster than the normal protocol. Of the tested microsatellite loci, 11 were successful in amplifying sharp and high-intensity bands in all the four multiplex panels and were polymorphic. The level of polymorphism ranged from 0.115 to 0.794, with a mean 0.50 and mean number of alleles varied from 2 to 10, with overall mean of 6 alleles per locus. The mean observed and expected heterozygosity ranged from 0.058 to 0.970 and 0.102 to 0.796, respectively. The 11 microsatellite loci that were effectively amplified from A. mearnsii DNA were adequate in detecting genetic variation among the tested populations. Conclusions: These PCR-based, multi-allelic, co-dominant microsatellite markers provide a powerful tool for genetic, breeding and conservation studies in A. mearnsii.

Colloidal stability of nanoparticles stabilized with mixed ligands in solvents with varying polarity

For gold nanoparticles stabilized with cetyl trimethylammonium bromide (CTAB) and polymer ligands, increase in solvent polarity leads to stabilization–aggregation–stabilization–aggregation transitions.

The Differential Spectroscopic Investigation of Partitioning of Reactive Dyes in Micellar Media of Cationic Surfactant, Cetyl Trimethylammonium Bromide (CTAB)

This manuscript reports the solubilization of two reactive dyes viz. reactive black-5 (RB-5) and reactive black-8 (RB-8) in the micellar media of cationic surfactant, cetyl trimethylammonium bromide (CTAB) by differential UV/visible spectroscopy. Absorption spectra of said dyes in the presence of CTAB provide strong evidence about dye–surfactant interaction. In premicellar region ion association pair is formed between dye and surfactant while in post micellar region dye molecules get accommodated within the micelles. The values of critical micelle concentration (CMC) of CTAB in the presence of dyes, partition coefficient; Kx, free energy of partition, ∆Gp, binding constant, Kb and free energy of binding, ∆Gb were determined and compared for both dyes. It is, thus, concluded that RB-5 is solubilized to greater extent than RB-8 because of its incorporation in palisade layer of micelle close to micelle-water interface.