Safety of food products intended for children: residual amounts of pesticides (literature review)

The literature review examines data on the assessment of contamination of food products and biological media with organochlorine pesticides, modern approaches, and methods for the identification and quantification of organochlorine pesticides (OCPs) as follows: hexachlorobenzene (HCB), aldrin, dieldrin, heptachlor, and endrin in various vegetable matrices, of animal origin (food products) and biological media (breast milk, blood serum, tissues). These pesticides, along with DDT and its metabolites and HCH (α, β, γ isomers), are persisting organic pollutants (POPs) according to the list of the Stockholm POPs Convention, included in the so-called global “dirty dozen substances”. Despite the prohibition of the production of these pesticides by most countries of the world community and the elimination of the quantities already produced, their migration along the food chain from contaminated objects of the environment to plants, and through plants to animals and humans, is still observed. Today’s contamination with OCPs and their metabolites remains a severe problem since they are detected in environmental objects, food products, and baby formula and breast milk. The literature review is based on virtual database platforms: Elsevier, PubMed, Science Direct, Medline, SciELO, Google Scholar, ResearchGate, e-LIBRARY, Electronic collection of legal and regulatory documents, and others.

Hygienic assessment of the skin-resorptive effect of xenobiotics (literature review)

The basis of safety for working with chemicals, including pesticides, is to minimize contact with them and prevent them from entering the body by inhalation and dermal route, sometimes in actual production conditions, the skin pathway can be more dangerous. The most studied inhalation route of harmful substances entering the body of a working person is confirmed by an extensive evidence base, the presence of regulatory and methodological documents, experimentally established and approved MPC in the air of the occupational area for 2484 substances, and 601 substances included in SanPiN 1.2.3685-211. The maximum permissible levels (MPL) on the skin are presented for only 26 substances in the same document. Identification and evaluation of the skin-resorptive effect of xenobiotics have methodological features due to the properties of the skin, which performs a barrier-protective function, and the physico-chemical, toxic properties of substances. The main stages of development in the domestic hygienic science and practice of research on the study and assessment of the skin-resorptive effect of xenobiotics in industrial contact with them are presented. The continuity of research on the study and evaluation of the skin-resorptive impact of chemicals used in various industries and agriculture is shown, making it possible to develop methodological approaches to the identification and risk assessment of dermal absorption of xenobiotics, including pesticides, within the organisms of workers. Evaluating the risk of skin contamination is the main argument for the development of measures for the primary prevention of skin resorptive action. Establishing the priority of the dermal route of toxicant intake is an evidence-based justification for making decisions about the possibility of using, for example, pesticides on the territory of the Russian Federation. The literature review is based on open sources hosted on virtual database platforms: Scopus, Web of Science, MedLine, Global Health, e-LIBRARY, Electronic Fund of Legal and Regulatory Documents, etc.

Odontogenic Keratocyst

Purpose: to review in detail various aspects of odontogenic keratocyst, emphasizing recent nomenclature, clinical, histopathological, recurrence, and management of odontogenic keratocyst. Methods: To achieve the objective of this review, a manual search was done in hard copy books of oral and maxillofacial pathology, and an electronic search was done in the google website, oral and maxillofacial pathology E-books, virtual database sites, such as PubMed, Research Gate, Academia, and Google scholar using the descriptors: odontogenic cyst, kerato odontogenic tumor, odontogenic keratocyst, and jaws cystic lesion. The eligibility criteria for selecting articles were: to be in the English language, studies published in journals, or indexed in these databases until 2021. Exclusion criteria were: articles in any languages other than English, studies presented in duplicate between the bases, whose theme did not contemplate the objective proposed in this review, or those not available in the digital environment. Data collection occurred from October to December 2020, followed by a thorough evaluation of the studies found, including an exploratory, selective, analytical, and interpretative reading. Summary and conclusions: the odontogenic keratocyst is noteworthy because of its unusual growth pattern, the tendency to recur, and association with an inherited syndrome. The renaming of odontogenic keratocysts as keratocystic odontogenic tumors has been one of the most debatable changes in the terminology of odontogenic lesions in recent years. Early diagnose of this lesion is important to perform the more conservative treatment. A wait-and-see policy, with yearly follow-up for the first five years and every two years after that, is strongly advocated.

Cardiovascular Regulation of the Elderly in Low Oxygenation Environments: A Narrative Review

Hypobaric hypoxia originates from the exposure of predominantly aerobic organisms to environments with low availability of atmospheric oxygen, however, prolonged exposures to hypoxic environments without previous experience or preparation can generate an atypical and possibly harmful physiological response to organic systems, mainly in older audiences. Thus, this study aimed to describe the main adaptive effects on cardiovascular control in hypoxic environments in elderly people with cardiovascular dysfunction. To carry out this study, a bibliographic survey was made on the virtual database Medline by PubMed, the protocol used to search for the studies was done through the selection of previously defined keywords. For the analysis of the data, the method of full description of the results of the selected works was adopted. It can be concluded that exposure to hypoxic environments directly and negatively influenced the cardiovascular regulation and cardiac behavior of the elderly, in which this episode worsened in the presence of comorbidities.

APPLICATION OF THE PRE-PROCESSOR MECHANISM FOR PROCESSING HETEROGENIOUS DATA IN DECISION-MAKING SUPPORT GEOINFORMATION SYSTEMS

The problem of local databases autonomy in distributed information systems is widely known and has been solved for a long time by specialists, but there is still no unambiguous and universally recog-nized approach to solving this problem. The process of working with distributed databases when creat-ing a geographic information system for decision support is not an exception, where the inviolability of the structure and completeness of local databases should remain the primary criterion for the sys-tem's operation. The purpose of the study is to increase the autonomy of local databases in geoinfor-mation decision support systems based on the use of preprocessing processing of heterogeneous data. This article proposes approaches and specific solutions for the physical and logical representation of the geoinformation system structure by means of the implementation of the preprocessing mechanism, which will allow you to combine and transform data even before they are displayed in the application. Thus, a person making a decision, when working in a geoinformational decision support system, will have full access to up-to-date information with the ability to record the results obtained by saving a virtual database created at his request.

Fragment-Based Drug Design of Antitumoral Molecules Polo-like kinase 1 inhibitors: in-silico approach

Background:: Kinase enzymes are reported to be very implicated in cancer. Polo-like kinase 1 (PLK1) is a protein kinase with a marked role in tumorigenesis and its inhibition is a promising anticancer therapeutic development strategy. Objective:: The purpose of this study was de novo design of new PLK1 inhibitors using in-silico approach. Method:: A virtual compounds library based on known inhibitors was designed using BREED algorithm. Molecules were geometry optimized then filtered according to lead-like properties using QiqProp. Receptor-ligand complex-based pharmacophore model was generated with Phase and used to virtually screen the new virtual database. Glide multistage molecular docking simulations were performed for the resulted compounds followed with a Prime MM-GBSA minimization. Results:: Two compounds (prd-comp 1-2) showed acceptable binding poses with a higher docking score than known inhibitor BI2536. MM-GBSA study confirmed that the leads have better binding energy than reference ligands. All leads bind to the key amino acids Cys133, Leu59, with a focus on molecule prd-comp1, proposed to have better affinity due to direct H-bond with Asp194. Conclusion:: Modifying hydration pattern of target protein by displacing water molecule is suggested to be a promising strategy for designing new PLK1 inhibitors. This applied methodology and the retrieved hits could be useful in the design of potent inhibitors of PLK1 as antitumoral agents.

Restoration of an 18th century frame: 3D modelling, printing and matching color of decorative flowers elements

This paper describes the application of 3D digital technology to support the rebuild of lost decorative elements of an historical frame. The frame belongs to an 18th century painting depicting the portrait of D. José, Prince of Brazil, displayed at the National Palace of Queluz, Portugal. The application of digital technologies has been planned from the very beginning in order to avoid the traditional use of silicone in the reproduction of missing elements. The photogrammetric 3D acquisition, a non-contact methodology, has been used to reduce the manipulation and hence prevent further degradation of the artistic object. Following the 3D modelling operation, new decorative elements were made using an additive printing technology. In the final phase of the work, the post-printing treatment was carried out, in which aqueous products were used. 3D modelling, in addition to facilitate the restoration treatment of the historic frame, can also be useful in the creation of a digital collection of decorative frame elements, i.e. a virtual database.

Symposium: Distributed Networks of Federated Secure Research Data Environments – Enabling Analytics Across Multiple Platforms

IntroductionUsers of linked data require access to an increasing number of heterogeneous datasets from diverse domains, often held in different secure research data environments, especially for multi-jurisdictional projects. Under the traditional model of data access, projects are required to transfer and harmonise the necessary datasets in one central location before analysis can be undertaken, increasing the time required for data acquisition and preparation. Objectives and ApproachIn a federated data environment, analysts query distributed datasets held in a network of multiple secure data environments via a central virtual database, without requiring the data to move. Instead, the data is analysed as close as possible to its storage location, minimising the amount of data transfers and giving data custodians more control over their data. This symposium explores the challenges and opportunities of establishing and operating a distributed network of federated secure research data environments. Leading organisations operating data platforms in various jurisdictions present for 15 minutes each the current capabilities of their platforms, the landscape of data environments in their jurisdictions and potential approaches to key questions such as: Harmonising/federating data sources Data security Data governance Discoverability/metadata Performance The audience is the then invited to participate in discussing the topic for the remaining 30 minutes. The following individuals have been approached to represent their organisations in this symposium: Professor David Ford, Swansea University: UK Secure eResearch Platform (UK SErP) Charles Victor, Institute for Clinical Evaluative Sciences (ICES): ICES Data & Analytic Virtual Environment (IDAVE) Professor Louisa Jorm, Centre for Big Data Research in Health, University of New South Wales: E-Research Institutional Cloud Architecture (ERICA) Professor Kimberlyn McGrail, Population Data BC: Secure Research Environment (SRE) Results / Conclusion / ImplicationsThis symposium will help formulate requirements for and barriers to distributed networks of federated secure research data environments, and create a foundation for data analytics across multiple platforms.

Subspecies-specific sequence detection for differentiation of Mycobacterium abscessus complex

Mycobacterium abscessus complex (MABC) is a taxonomic group of rapidly growing, nontuberculous mycobacteria that are found as etiologic agents of various types of infections. They are considered as emerging human pathogens. MABC consists of 3 subspecies—M. abscessus subsp. bolletti, M. abscessus subsp. massiliense and M. abscessus subsp. abscessus. Here we present a novel method for subspecies differentiation of M. abscessus named Subspecies-Specific Sequence Detection (SSSD). This method is based on the presence of signature sequences present within the genomes of each subspecies of MABC. We tested this method against a virtual database of 1505 genome sequences of MABC. Further, we detected signature sequences of MABC in 45 microbiological samples through DNA hybridization. SSSD showed high levels of sensitivity and specificity for differentiation of subspecies of MABC, comparable to those obtained by rpoB sequence typing.