flavocytochrome b
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2017 ◽  
Vol 250 ◽  
pp. 469-475 ◽  
Author(s):  
Oleh Smutok ◽  
Maria Karkovska ◽  
Roman Serkiz ◽  
Вohdan Vus ◽  
Narimantas Čenas ◽  
...  

2012 ◽  
Vol 521 (1-2) ◽  
pp. 24-31 ◽  
Author(s):  
Ross M. Taylor ◽  
Marcia H. Riesselman ◽  
Connie I. Lord ◽  
Jeannie M. Gripentrog ◽  
Algirdas J. Jesaitis

2009 ◽  
Vol 20 (5) ◽  
pp. 1520-1532 ◽  
Author(s):  
Xing Jun Li ◽  
Wei Tian ◽  
Natalie D. Stull ◽  
Sergio Grinstein ◽  
Simon Atkinson ◽  
...  

The assembly of cytosolic p47phoxand p67phoxwith flavocytochrome b558at the membrane is crucial for activating the leukocyte NADPH oxidase that generates superoxide for microbial killing. p47phoxand p67phoxare linked via a high-affinity, tail-to-tail interaction involving a proline-rich region (PRR) and a C-terminal SH3 domain (SH3b), respectively, in their C-termini. This interaction mediates p67phoxtranslocation in neutrophils, but is not required for oxidase activity in model systems. Here we examined phagocytosis-induced NADPH oxidase assembly, showing the sequential recruitment of YFP-tagged p67phoxto the phagosomal cup, and, after phagosome internalization, a probe for PI(3)P followed by a YFP-tagged fragment derived from the p47phoxPRR. This fragment was recruited in a flavocytochrome b558-dependent, p67phox-specific, and PI(3)P-independent manner. These findings indicate that p47PRR fragment probes the status of the p67phoxSH3b domain and suggest that the p47phox/p67phoxtail-to-tail interaction is disrupted after oxidase assembly such that the p67phox-SH3b domain becomes accessible. Superoxide generation was sustained within phagosomes, indicating that this change does not correlate with loss of enzyme activity. This study defines a sequence of events during phagocytosis-induced NADPH oxidase assembly and provides experimental evidence that intermolecular interactions within this complex are dynamic and modulated after assembly on phagosomes.


2008 ◽  
Vol 22 (S2) ◽  
pp. 552-552
Author(s):  
Amy Jo Casbon ◽  
Kenneth W. Dunn ◽  
Lee‐Ann Allen ◽  
Mary C. Dinauer

2008 ◽  
Vol 329 (1-2) ◽  
pp. 201-207 ◽  
Author(s):  
Connie I. Lord ◽  
Marcia H. Riesselman ◽  
Jeannie M. Gripentrog ◽  
James B. Burritt ◽  
Algirdas J. Jesaitis ◽  
...  

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