Evaluation of Host Stinging Behavior of the Larval Ectoparasitoid Diglyphus isaea on Some Selected Host Plants

Background: In parasitoid insects, beside parasitism, there are two other behaviors that may increase leaf miner mortality: Feeding on the host (host feeding) and, host penalization without oviposition or feeding (host stinging). Objectives: The present study aimed to evaluate (host stinging) behavior of the larval ectoparasitoid D. isaea in field on some selected host plants. Methods: The present study was carried out in Alejelat region. Four selected host plants were studied, chick pea (Cicer arietinum) and broad bean (Vecia faba) as a Winter host plants and, Cow pea (Vigna unguiculate) and kidney bean (Phaseolus vulgaris) were selected as summer host plants during the growing seasons 2018 and 2019. Samples were kept in plastic bags and transferred to be examined in the laboratory. Number of living L. trifolii larvae, and number of killed larvae according to feeding (no oviposition) by D. isaea were counted and recorded. Results: On cow pea host stung larvae recorded (156 individuals/ 100 infested leaflets) occurred in 10th of August, with (r = +0.69**) between host stung larvae and the population of L. trifolii recorded). On kidney bean host stung larvae recorded (101 individuals/ 100 infested leaflets) occurred in 24th of August, with (r = + 0.85**) value between host stung larvae and the population of L. trifolii recorded. On the other hand, the percentage of host stung larvae ranged between (5.67% and 28.22%) and (15.88% and 42.30%) for cow pea and kidney bean respectively. On broad bean host stung larvae recorded (159 individuals/ 100 infested leaflets), with (r = +0.59*) between host stung larvae and the population of L. trifolii .On chick pea host stung larvae recorded (82 individuals/ 100 infested leaflets) with (r = +0.20ns) between host stung larvae and the population of L. trifolii. On the other hand, percentages of host stung larvae ranged between (8.45% and 36.60%) and (21.80% and 87.65%) for broad bean and chick pea respectively. Conclusion: The highest percentage of host stung larvae recorded on chick pea 44.65% followed by kidney bean 24.21%, broad bean 18.45% and cow pea 12.97% respectively. On the other hand, the correlation coefficient value (r) between numbers of L. trifolii larvae and, host stung larvae recorded the highest on kidney bean +0.85** followed by cow pea +0.69**, Broad bean +0.59* and Chick pea +0.20ns respectively.

A Novel Optimization Algorithm Based on Stinging Behavior of Bee

<span lang="EN-US">Optimization algorithms are search methods to find an optimal solution to a problem with a set of constraints. Bio-Inspired Algorithms (BIAs) are based on biological behavior to solve a real world problem. BIA with optimization technique is to improve the overall performance of BIA. The aim of this paper is to introduce a novel optimization algorithm which is inspired by natural stinging behavior of honey bee to find the optimal solution. This algorithm performs both monitor and sting if any occurrence of predators. By applying a novel optimization algorithm based on stinging behavior of bee, used to solve the intrusion detection problems. In this paper, a new host intrusion detection system based on novel optimization algorithm has been proposed and implemented. The performance of the proposed Anomaly-based Host Intrusion Detection System (A-HIDS) using a novel optimization algorithm based on stinging behavior of bee has been tested. In this paper, after an explanation of the natural stinging behavior of honey bee, a novel optimization algorithm and A-HIDS are described and implemented. The results show that the novel optimization algorithm offers some advantage according to the nature of the problem.</span>

Quantitative Trait Loci for Honey Bee Stinging Behavior and Body Size

A study was conducted to identify quantitative trait loci (QTLs) that affect colony-level stinging behavior and individual body size of honey bees. An F1 queen was produced from a cross between a queen of European origin and a drone descended from an African subspecies. Haploid drones from the hybrid queen were individually backcrossed to sister European queens to produce 172 colonies with backcross workers that were evaluated for tendency to sting. Random amplified polymorphic DNA markers were scored from the haploid drone fathers of these colonies. Wings of workers and drones were used as a measure of body size because Africanized bees in the Americas are smaller than European bees. Standard interval mapping and multiple QTL models were used to analyze data. One possible QTL was identified with a significant effect on tendency to sting (LOD 3.57). Four other suggestive QTLs were also observed (about LOD 1.5). Possible QTLs also were identified that affect body size and were unlinked to defensive-behavior QTLs. Two of these were significant (LOD 3.54 and 5.15).