Using the Idexx SediVue Dx to predict the need for urine bacteriologic culture in cats

We analyzed urine samples from 191 cats for bacteriuria with an automated urine sediment analyzer (Idexx SediVue Dx), combined with image review by an observer, and compared to bacteriologic culture results. Sixty-nine samples were unambiguously assigned to be free of bacteria by the instrument and the observer, and no bacterial growth was detected. Twenty-seven samples were unambiguously assigned to have bacteriuria; 24 of these 27 samples were culture-positive. For these samples, bacteriuria was predicted with a sensitivity of 100% and a specificity of 96%. A clear assignment was not possible for 95 samples, 81 of which were culture-negative. Specificity dropped to 45% when all samples were considered. Using the automated leukocyte count to predict bacteriuria, sensitivity was 82% and specificity was 75%. Automated sediment analysis is faster and less observer-dependent than sediment analysis under a microscope, but accurate detection of bacteriuria remains difficult in a large proportion of samples. Bacteriuria was significantly associated with leukocyte count; the leukocyte count was >5/high power field in 82% of culture-positive samples.

Changes in Particle Size, Sedimentation, and Protein Microstructure of Ultra-High-Temperature Skim Milk Considering Plasmin Concentration and Storage Temperature

Age gelation is a major quality defect in ultra-high-temperature (UHT) pasteurized milk during extended storage. Changes in plasmin (PL)-induced sedimentation were investigated during storage (23 °C and 37 °C, four weeks) of UHT skim milk treated with PL (2.5, 10, and 15 U/L). The increase in particle size and broadening of the particle size distribution of samples during storage were dependent on the PL concentration, storage period, and storage temperature. Sediment analysis indicated that elevated storage temperature accelerated protein sedimentation. The initial PL concentration was positively correlated with the amount of protein sediment in samples stored at 23 °C for four weeks (r = 0.615; p < 0.01), whereas this correlation was negative in samples stored at 37 °C for the same time (r = −0.358; p < 0.01) due to extensive proteolysis. SDS-PAGE revealed that whey proteins remained soluble over storage at 23 °C for four weeks, but they mostly disappeared from the soluble phase of PL-added samples after two weeks’ storage at 37 °C. Transmission electron micrographs of PL-containing UHT skim milk during storage at different temperatures supported the trend of sediment analysis well. Based on the Fourier transform infrared spectra of UHT skim milk stored at 23 °C for three weeks, PL-induced particle size enlargement was due to protein aggregation and the formation of intermolecular β-sheet structures, which contributed to casein destabilization, leading to sediment formation.

Between Natural and Anthropogenic Coastal Landforms: Insights from Ground Penetrating Radar and Sediment Analysis

Both natural and anthropogenic coastal landforms characterize Penang Island. As years have passed it is a challenge to differentiate the genuineness of landmasses created by natural geological formations or by coastal reclamation projects. An account is given of the environmental impact of solid wastes used for reclaiming land in coastal areas of Penang and of the impact of a major sewage outfall in the western channel. Leaching of heavy metals was shown to be one of the main sources of contamination from solid wastes. This paper presents eight lines of ground penetrating radar (GPR) surveys and sediment analysis to identify the anthropogenic interventions that shaped the urban landscape of Penang Island by excavations, filling, and embankment construction along the coastline and differentiate it from the natural one. The surveys were implemented in two locations, the Batu Ferringhi area, representing the natural coastline, and Persiaran Bayan Indah (the Queensbay Mall area), representing the anthropogenic coastal landform. The apparent depth of penetration that was achieved using a 250-MHz antenna is limited (less than 5 m). The results show between natural and anthropogenic sediment recorded different radar facies. In complement mode, mean grain size distribution, sorting, skewness, and kurtosis graphics of sediment samples from both sites correspond with the GPR data. This technique can likely be applied to the developing coast, where natural and anthropogenic coastal landform data is incomplete, considering future coastline development.

Sediment analysis and modelling reveal short inundation distances and low onshore flow speed of the 2018 Palu-Donggala tsunami in Indonesia

&lt;p&gt;On 28 September 2018, a magnitude 7.5 earthquake struck north of Palu, Central Sulawesi, Indonesia. The earthquake generated a tsunami with inundation depths of up to 7.5 m and run-up of up to 10 m above sea level. Inundation distances were only partly topography dependent and reached less than 400 m inland even where terrain did not rise steeply beyond that point. A subsequent tsunami was generated by a combination of minor fault displacement and multiple submarine landslides. In places, co-seismic coastal subsidence of &gt;1 m exacerbated the tsunami inundation. During a post-event field survey in November 2018, we sampled three transects for sediment analysis; two in Palu City and one on the eastern coast of Palu Bay. The tsunami deposits in Palu City are predominantly massive, fine- to medium-grained sand in thin layers (&lt;5 cm) with patchy distribution of sediments. In contrast, sediments present near Pantoloan on the east coast of Palu Bay were coarser (medium- to coarse-grained sand), thicker (up to 12 cm) and more continuous. These tsunami deposits exhibited fining and thinning landwards, and are characterized by a continuous sand sheet that extends up to 250 m inland with few post depositional changes. The grain size ranges from coarse-grained sand to silty-fine-grained sand at the landward extent. The Pantoloan site also contained wave-transported blocks of sea wall weighing up to 4.7 t in addition to sandy deposits. The blocks together with grain size data suggest that water velocities reached 3 m.s&lt;sup&gt;-1&lt;/sup&gt; at more than 130 m from the coast. The tsunami deposits of Palu Bay generally exhibit sedimentological and stratigraphic characteristics shared by storm and tsunami deposits, which maybe be ascribed to the short wave length, relatively low power and short-term inundation of the tsunami and the limited availability of sediments in the nearshore environment.&lt;/p&gt;