myoblast culture
Recently Published Documents


TOTAL DOCUMENTS

7
(FIVE YEARS 0)

H-INDEX

3
(FIVE YEARS 0)

2013 ◽  
Vol 24 (16) ◽  
pp. 1831-1847 ◽  
Author(s):  
Giovanna Della Porta ◽  
Nunzia Falco ◽  
Emanuele Giordano ◽  
Ernesto Reverchon

2011 ◽  
Vol 76 (3) ◽  
pp. 359-365 ◽  
Author(s):  
I. I. Ermakova ◽  
G. A. Sakuta ◽  
M. A. Potekhina ◽  
M. A. Fedorova ◽  
R. Hoffmann ◽  
...  

2001 ◽  
Vol 91 (1) ◽  
pp. 63-74 ◽  
Author(s):  
Pascale Boudreault ◽  
Jacques P. Tremblay ◽  
Marie-France Pépin ◽  
Alain Garnier

1998 ◽  
Vol 90 (1) ◽  
pp. 88
Author(s):  
L. Behrens ◽  
S. Maier ◽  
E.H. Weiss ◽  
R. Hohlfeld

1991 ◽  
Vol 69 (1) ◽  
pp. 49-52 ◽  
Author(s):  
Qiuwen Fang ◽  
Ming Chen ◽  
Hua-ju Li ◽  
Tena G. Goodwin ◽  
Peter K. Law

A new method is developed using Fluoro-Gold (FG) as a vital stain to label the nuclei of donor myoblasts in myoblast transfer studies. In vitro incubation with 0.01% FG for 16 h resulted in 100% nuclei labelling. Intensive fluorescence persisted following 9 days of subculture, when the human myoblasts were injected into the quadriceps of mouse recipients immunosuppressed with cyclosporine. Injected muscles showed mosaicism of host and donor nuclei 25 days after injection, indicating (i) survival and fusion among donor myoblasts, and (ii) fusion between host and donor cells. FG labelling was not observed in control muscles injected with an equal volume of FG-labelled dead myoblasts, 0.01% FG medium, or phosphate-buffered saline.Key words: Fluoro-Gold, nucleus vital stain, myoblast culture and transplant.


Sign in / Sign up

Export Citation Format

Share Document