The Reconfigured Body. Human-animal relations in xenotransplantation

<span style="font-family: MeliorLTCE; font-size: x-small;"><span style="font-family: MeliorLTCE; font-size: x-small;"><p>The article explores issues concerning the reconfiguration of human and animal bodies in modern biotechnology. The examples are based on xenotransplantation: Transplantation of cells, tissue and organs from animals to humans. Three thematic issues that emerged from xenotransplantation research in Sweden in the 1990s and early 2000s are examined in the article. The first issue concerns how the pig was introduced as a donor animal in xenotransplantation and, at the same time, dehumanized in relation to what is human. Baboons and chimpanzees that had previously been used in xenotransplantation now became an ethically problematic choice, and were in stead humanized. The second issue concerns the introduction of transgenic and cloned pigs as commoditized objects. The biotechnological development reconfigured the pig’s cells, tissue and organs to become more human-like. The third issue concerns the risk that pigs contain retrovirus that could infect the transplanted patients. The human body became part of a network of both animal and retrovirus. Boundlessness between human and animal bodies appears in these three thematic phases and is analysed from a cultural perspective.</p></span></span>

Effect of donor cell age on development of ovine nuclear transfer embryos in vitro

SummaryThe effects of the age of cell donor animal on in vitro development of ovine nuclear transfer (NT) embryos were investigated. Somatic donor cells were obtained from two different sources: (1) adult cells (adult fibroblast cells; AFC and adult cumulus cells; ACC); and (2) fetal fibroblasts (40-day-old; FFC-40 and 65-day-old; FFC-65). The fibroblast cell lines were used for NT procedures within 4–13 subpassages. While the cumulus cells were used as non-cultured (fresh) cells. The in vitro matured abattoir-derived oocytes were considered as recipients. No differences in the rates of fusion (75.7, 77.7, 76.3 and 86.7%) and cleavage (80.1, 84.3, 77.8 and 74%) were detected among couplets reconstructed with FFC-40, FFC-65, AFC and ACC, respectively. Blastocyst formation rate of those oocytes reconstructed with FFC-40 was higher (18%; p < 0.001) than those reconstructed with FFC-65 (13%) and AFC (10.9) and comparable with those reconstructed with ACC (17.5%). When the effect of passage number was analysed within groups (FFC-40, FFC-65 and AFC) there were no significant differences in fusion, cleavage and blastocyst rates between reconstructed oocytes. The present study demonstrates that the fetal and adult fibroblasts as well as fresh cumulus cells are comparable in their ability to attain cell fusion and embryonic cleavage. Moreover, the blastocyst formation rate is influenced by the age of the donor animal and the fresh cumulus cells have similar remodelling potential to that of fetal fibroblasts in term of blastocyst formation rate.

The effect of feeding a low- or a high-starch diet on thein vitrofermentative capacity of equine faecal inocula

Seven mature Welsh-cross pony geldings provided the faecal inocula in a cross-over design experiment, consisting of two 14-day periods. In period 1, four ponies (group 1) were offered a low-starch fibre mix (LS), and three (group 2) were offered a conventional high-starch coarse mix (HS). Both groups were offered these mixes in a 50:50 ratio with mature grass hay, to give a total daily dry-matter intake of 17·5 g/kg live weight per day. Diets were then switched in period 2. At the end of each experimental period freshly voided faeces were collected from each animal and assessed for their ability to ferment grass hay (H), fibre mix (FMix) or starch-based coarse mix (SMix) using the gas production (GP) technique of Theodorouet al.(1994). Donor animal diet and donor animal had no effect on any end-point measurements. Lag times recorded for the SMix were significantly (P<0·001) greater in LS-inoculated bottles compared with the HS inocula (1·74v.2·25 h, respectively). Lag times for FMix and SMix varied significantly (P<0·001) between ponies (0·82 to 1·78 h in the FMix and 1·64 to 2·51 h in the SMix). The degradation rate of H also differed significantly (P<0·001) between ponies with the time taken to reach 50% of GP (T50) ranging from 12·70 to 17·30 h. Consequently, it would appear that the effect of feeding LS or HS on thein vitrofermentative capacity of equine faecal inocula is minimal; moreover, the GP technique appears to be valuable tool for evaluating such effects.

The use of faecal inocula for estimating the in vitro digestibility of horse feeds

There is a dearth of information available on the effects of donor animal on the fermentative capacity of equine faecal inocula for use in in vitro digestibility determinations. Furthermore, there is little knowledge of the degradation characteristic of feedstuffs incubated with equine faecal inocula. As such this study aimed to elucidate the effect of donor animal on the fermentation of feedstuffs in vitro and to assess the in vitro degradation characteristics of three commonly fed components of horse diets incubated with equine faecal inocula.

Heterotopic uterine transplantation by vascular anastomosis in the mouse

A method of heterotopic uterine transplantation was developed in the mouse as a model system for studies of uterine function and transplant immunology of the uterus. The model involved transplantation of the right uterine horn and the cervix by vascular anastomosis to a donor animal with the intact native uterus remaining in situ. F1-hybrids of inbred C57BL/6 x CBA/ca (B6 CBAF1) mice of 6-8 weeks of age (n=42) were used. The specific pelvic vascular anatomy of these mice was first examined by intra-aortal injection of a two-component silicon-rubber curing agent. The surgery of the donor animal involved microsurgical isolation of the right uterine horn and the cervix, with preserved vascular supply from the aorta through the right uterine artery. After isolation of the uterine horn with vascular supply and venous drainage, including approximately 3 mm of the inferior vena cava and aorta, the organ was put on ice. The recipient animal was prepared by exposing and mobilizing the infrarenal part of the aorta and the vena cava. The grafted uterus was placed in the abdomen on the left side and the aorta and vena cava of the graft were anastomosed end-to-side to the aorta and vena cava of the recipient animal with 11-0 sutures. The total time for these procedures declined with time and was 125+/-4 min for the last 28 operations. Viability of the uterus was confirmed, several days later, by demonstrating a blood flow similar to that of the native uterus, and histology of the grafted uterus demonstrated normal morphology, including intact ultrastructure of the endothelial cells. The overall survival rate of the recipient animals increased with learning from approximately 40% in animals 1-21 to 71% in animals 22-42. The proportion of viable grafts, as judged by normal blood flow and histology among the surviving mice was 25% in animals 1-21 and 87% in animals 22-42. An undisturbed function of the transplanted uterus horn was finally demonstrated by its ability to implant inserted blastocysts and to carry pregnancy with fetal weight being similar to that of fetuses in the native uterus and controls. In conclusion, this is the first report of successful transplantation of the uterus with proven functionality in the mouse. The model should be useful for many aspects of research in uterine physiology and pathophysiology.

Effect of tropical diets on inocula used on in vitro gas production technique

There is much discussion about the effect of the diet of the inoculum donor animal. The ideal diet should supply microrganisms and they should be able to degrade the feed. But when we evaluate several different feeds by a gas production assay, it is very difficult to feed donor animals with a diet composed by all feeds that will be tested. The aim of this work was to investigate the effect of three different tropical diets on inoculum ability to degrade the feeds.