Anaerobic 3-methylhopanoid production by an acidophilic photosynthetic purple bacterium

Bacterial lipids are well-preserved in ancient rocks and certain ones have been used as indicators of specific bacterial metabolisms or environmental conditions existing at the time of rock deposition. Here we show that an anaerobic bacterium produces 3-methylhopanoids, pentacyclic lipids previously detected only in aerobic bacteria and widely used as biomarkers for methane-oxidizing bacteria. Both Rhodopila globiformis, a phototrophic purple nonsulfur bacterium isolated from an acidic warm spring in Yellowstone, and a newly isolated Rhodopila species from a geochemically similar spring in Lassen Volcanic National Park (USA), synthesized 3-methylhopanoids and a suite of related hopanoids and contained the genes encoding the necessary biosynthetic enzymes. Our results show that 3-methylhopanoids can be produced under anoxic conditions and challenges the use of 3-methylhopanoids as biomarkers of oxic conditions in ancient rocks and as prima facie evidence that methanotrophic bacteria were active when the rocks were deposited.

Anaerobic 3-Methylhopanoid Production By An Acidophilic Phototrophic Purple Bacterium

Bacterial lipids are well preserved in ancient rocks and certain ones have been used as indicators of specific bacterial metabolisms or environmental conditions existing at the time of rock deposition. Here we show that an anaerobic bacterium produces 3-methylbacteriohopanepolyols (3-MeBHPs), pentacyclic lipids previously detected only in aerobic bacteria and widely used as biomarkers for methane-oxidizing bacteria. Both Rhodopila globiformis, a phototrophic purple nonsulfur bacterium isolated from an acidic warm spring in Yellowstone, and a newly isolated Rhodopila species from a geochemically similar spring in Lassen Volcanic National Park (USA), synthesized 3-MeBHPs and a suite of related BHPs and contained the genes encoding the necessary biosynthetic enzymes. Our results show that 3-MeBHPs can be produced under anoxic conditions and challenges the use of 3-MeBHPs as biomarkers of oxic conditions in ancient rocks and as prima facie evidence that methanotrophic bacteria were active when the rocks were deposited.

Contrasting prescription burning and wildfires in California Sierra Nevada national parks and adjacent national forests

History of prescription burning and wildfires in the three Sierra Nevada National Park Service (NPS) parks and adjacent US Forest Service (USFS) forests is presented. Annual prescription (Rx) burns began in 1968 in Sequoia and Kings Canyon National Parks, followed by Yosemite National Park and Lassen Volcanic National Park. During the last third of the 20th century, USFS national forests adjacent to these parks did limited Rx burns, accounting for very little area burned. However, in 2004, an aggressive annual burn program was initiated in these national forests and in the last decade, area burned by planned prescription burns, relative to area protected, was approximately comparable between these NPS and USFS lands. In 1968, the NPS prescription burning program was unique because it coupled planned Rx burns with managing many lightning-ignited fires for resource benefit. From 1968 to 2017, these natural fires managed for resource benefit averaged the same total area burned as planned Rx burns in the three national parks; thus, they have had a substantial impact on total area burned by prescription. In contrast, on USFS lands, most lightning-ignited fires have been managed for suppression, but increasing attention is being paid to managing wildfires for resource benefit.