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2015 ◽  
Vol 735 ◽  
pp. 220-225
Author(s):  
Rozita Nasiri ◽  
Javad Hamzehalipour Almaki ◽  
Sara Sheibani Esfarjani ◽  
Chan Giek Far ◽  
Noor Aini Abdul Rashid

In this study, PCR amplification of biodegradation related genes from NAR-2 bacterial consortium was accomplished. NAR-2 bacterial consortium consists of Citrobacter freundii A1, Enterococcus casseliflavus C1 and Enterobacter cloacae L17. The amplified genes were sequenced, bioinformatically analyzed and compared with the sequences from GenBank database of National Centre of Biotechnology Information (NCBI) using BLASTn and BLASTp search tools. The assembled sequences represented almost >70% of similarity to biodegradation related genes. These genes may act as a key intermediate enzyme in biodegradation pathway.


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