vertebrate limb development
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Biosystems ◽  
2021 ◽  
pp. 104502
Author(s):  
Stuart A. Newman ◽  
Ramray Bhat ◽  
Tilmann Glimm

2018 ◽  
Author(s):  
Alison Cloutier ◽  
Timothy B. Sackton ◽  
Phil Grayson ◽  
Scott V. Edwards ◽  
Allan J. Baker

AbstractHigh throughput sequencing (HTS) has revolutionized the field of ancient DNA (aDNA) by facilitating recovery of nuclear DNA for greater inference of evolutionary processes in extinct species than is possible from mitochondrial DNA alone. We used HTS to obtain ancient DNA from the little bush moa (Anomalopteryx didiformis), one of the iconic species of large, flightless birds that became extinct following human settlement of New Zealand in the 13 th century. In addition to a complete mitochondrial genome at 249.9X depth of coverage, we recover almost 900 Mb of the moa nuclear genome by mapping reads to a high quality reference genome for the emu (Dromaius novaehollandiae). This first nuclear genome assembly for moa covers approximately 75% of the 1.2 Gb emu reference with sequence contiguity sufficient to identify more than 85% of bird universal single-copy orthologs. From this assembly, we isolate 40 polymorphic microsatellites to serve as a community resource for future population-level studies in moa. We also compile data for a suite of candidate genes associated with vertebrate limb development and show that the wingless moa phenotype is likely not attributable to gene loss or pseudogenization among this candidate set. We also identify potential function-altering coding sequence variants in moa for future experimental assays.


2014 ◽  
Vol 76 (7) ◽  
pp. 471-475 ◽  
Author(s):  
Jennifer O. Liang ◽  
Matthew Noll ◽  
Shayna Olsen

We have developed an upper-level undergraduate laboratory exercise that enables students to replicate a key experiment in developmental biology. In this exercise, students have the opportunity to observe live chick embryos and stain the apical ectodermal ridge, a key tissue required for development of the vertebrate limb. Impressively, every student who has tried this protocol has been successful, making it a good introduction to the use of the chick model system in studying development. The array of materials about limb development, using chick embryos in teaching laboratories, and the history of this experiment provide a rich background for teachers and students.


2013 ◽  
Vol 243 (1) ◽  
pp. 1-17 ◽  
Author(s):  
Yong-Tao Zhang ◽  
Mark S. Alber ◽  
Stuart A. Newman

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