amber mutant
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Genetics ◽  
1996 ◽  
Vol 144 (4) ◽  
pp. 1337-1341 ◽  
Author(s):  
Michael J Prival ◽  
Thomas A Cebula

We have studied revertants, selected on lactose minimal agar medium, of the Escherichia coli lacZ  am strain that was first used by Cairns and his colleagues to demonstrate the phenomenon of “adaptive mutation.” We have found, by performing appropriate reconstruction studies, that most of the late-arising Lac+ revertants of this lac amber strain (appearing as colonies in 3–5 days) are slow-growing ochre suppressor mutants that probably existed in the culture prior to plating and cannot, therefore, be classified as “adaptive.” The appearance of a small number of fast-growing, late-arising Lac+ revertants may result from residual cell growth and turnover or from phenomena related to the fact that the lacZ  am mutation in strain SM195 is carried on an F′ plasmid. Thus, the appearance of late-arising revertants in this lacZ  am system does not provide convincing evidence that selective conditions specifically increase the rate of occurrence of favorable mutations.


1983 ◽  
Vol 58 (1) ◽  
pp. 86 ◽  
Author(s):  
J. A.
Keyword(s):  

1981 ◽  
Vol 183 (1) ◽  
pp. 66-73 ◽  
Author(s):  
Hiroyuki Araki ◽  
Hideyuki Ogawa

1976 ◽  
Vol 106 (4) ◽  
pp. 963-981 ◽  
Author(s):  
David McCarthy ◽  
Charles Minner ◽  
Harris Bernstein ◽  
Carol Bernstein
Keyword(s):  
Phage T4 ◽  

1976 ◽  
Vol 106 (3) ◽  
pp. 673-682 ◽  
Author(s):  
Melvin Sunshine ◽  
Erich Six ◽  
Kathleen Barrett ◽  
Richard Calendar

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