Cytogenetic effect of some nanostructure polymers prepared via gamma irradiation on Vicia faba plant

Background Nourishment plants during the field time is a must; to have healthy, high productive and self-propagating plants. The trendy nano-fertilizers came to the front in modernized agriculture seeking for minimizing the soil suffocation with other chemical fertilizers in the bulk size. Nano-fertilizers may represent a way out of shot as they are completely absorbed by plant due to their small size, also it magnifies the benefit to the plant due to its high surface area. Nano-fertilizers are introduced via different way of synthesis methods. In this work, three of new nanocomposites are prepared in nano form via Gamma irradiation from Cobalt 60 source at irradiation dose 5 KGy. These composites which can supply plants with P, Zn elements needs to be revised for their safety usage in agriculture. Methodology Three compounds; Zinc oxide, phosphorous and the mixed Zinc–phosphorous elements were prepared in nano-composite forms coated with PVP as a shell and then characterized by HR-TEM, UV and FT-IR to emphasize their new sizes and shapes, then, they were examined for their cytotoxicity in three concentrations (0.5, 1 and 2%) on Vicia faba plants; after 3 h of direct roots treatment. Cytotoxicity test concerned the mitotic index, phase index, abnormal mitosis and the type of the aberrations at each phase. Results The three tested NPs exerted mito-accelerating effect on root meristematic cells. However, concentration‐dependent genotoxicity was also an evident. Conclusion The three examined nano-composites may recommend to be used in the lowest examined concentrations to minimize its harm effect on the plant cell and keep their benefits to the environment. It also recommended to count the Zn/P mix NPs over ZN or P separately as it induces an intermediating cytogenetic effect on mitosis apparatus of Vicia faba plant. Graphical Abstract

Critical Analysis of Snuhi - Euphorbia Neriifolia (Upavisha- Poisonous Plant) and its Application as a Therapeutic Aid

Traditional system of medicines has vast applications of poisonous plants; hence the knowledge about poisonous plant and its therapeutic effects becomes very essential to develop a new drug for the new diseases like Covid 19 and other diseases. Many research studies have showed that the poisonous medicinal plants are endowed with properties like Antidiabetic, Anticancer, Antibacterial, Antifungal, and Cytogenetic effect. This study focused on one such drug called Snuhi - Euphorbia Neriifolia, a Upavisha or a poisonous plant to evaluate its therapeutic effects, which would be helpful for the mankind after the proper Shodhana (purification), by conducting experimental studies, toxicity studies and thereby applied in various diseases for its therapeutic benefits and to relieve the sufferings of the patients.

On Some Methodological Issues of Studying Cytogenetic Effects in Cancer Patients Treated with Neutron Therapy Using U-120 Cyclotron

Purpose: To study dosimetric characteristics of neutron radiation field, to determine their role in the formation of the total cytogenetic effect in the patient’s body and to assess the cytogenetic dosimetry capabilities in improving the quality of NT. Material and methods: A therapeutic beam with the average neutron energy of ~6.3 MeV was obtained from the V-120 cyclotron. The radiation field of the beam was investigated with the help of two ionization chambers with different sensitivity to neutrons. Chamber with high and low sensitivities were made of polyethylene and graphite, respectively. To exclude the uncertainty associated with the change in beam intensity in time, a dosimeter monitor operating in the integral mode was used. Results: The dependence of the monitor factor on the irradiated area was measured. The distributions of the absorbed dose of neutrons and γ-radiation over the depth of the tissue-equivalent medium were found. The contribution of γ-radiation to the neutron dose was increased from ~10 % at the entry to the medium to ~30 % at a depth of 16 cm. Dose distributions of scattered neutron and γ-radiation in the plane of the end face of the forming device were obtained. The contribution of these radiations to the dose received by the patient’s body was estimated. This contribution was shown to be comparable with that from the therapeutic beam. The analysis of the influence of NT on the estimation of the frequency of chromosome aberrations in the blood of patients was carried out. Conclusion: The frequency of chromosome aberrations in the blood of patients was determined by the whole-body dose, including dose due to scattered radiation. When using equal focal doses, the cytogenetic effect was found to be dependent on the area of the irradiated field and the depth of the tumor in the patient’s body. The differences in the RBE of neutrons and γ-radiation as well as the instability of the therapeutic neutron beam intensity create uncertainties that do not allow for the necessary control over the doses using the cytogenetic dosimetry. Therefore, cytogenetic dosimetry should be combined with an effective instrument dosimetry method. The use of biodosimetry based on the assessment of the frequency of chromosome aberrations is promising for controlling the average whole-body dose, on which the overall radiation response of the body depends.

Investigation of In-ovo cytogenetic effect of Levofloxacin

This study was carried out to investigate the cytogenetic effect of Levofloxacin after In-ovo inoculation. Forty eight fertilized eggs were used, divided equally into eight groups and inoculated through shell puncture above air cell with materials in equestion and incubated for two different periods three and seven days. They were assigned as group one and group two 10 µg Levofloxacin, group three and group four 20µg levofloxacin, group five and group six considered positive control 0.05 µg Mitomycin-C, group seven and group eight (Phosphate Buffer Saline) considered negative control groups. All eggs from in-ovo experiments were subjected to cytogenetic tests, such as Mitotic Index, Replicative Index and Sister Chromatid exchange. Eighty fertilized eggs were handled with the same manner of in-ovo inoculation and used for calculation Hatchability index and weekly body weight. The results revealed significant (P˂0.05) increases in Mitotic Index and Replicative Index of all groups treated with levofloxacin compared to positive control, but there were significant (P˂0.05) decreases compared to control negative. There were no significant changes in Sister Chromatid exchange of all treated levofloxacin in–ovo. The hatchability index revealed significant (P˂0.05) decreases in group three which was inoculated 20 µg levofloxacin after three days of incubation compared to all Levofloxacin treated groups and both positive and negative control groups. In conclusion, levofloxacin in–ovo inoculation has no substantial cytogenetic effects.