Operation of Submerged Anaerobic Membrane Bioreactors at 20 °C: Effect of Solids Retention Time on Flux, Mixed Liquor Characteristics and Performance

Four flat-sheet submerged anaerobic membrane bioreactors ran for 242 days on a simulated domestic wastewater with low Chemical Oxygen Demand (COD) and high suspended solids. Organic loading was maintained around 1.0 g COD L−1 day−1, while solids retention time (SRT) was varied from 20–90 days. This was achieved at a constant membrane flux, maintained by adjusting transmembrane pressure (TMP) in the range 1.8-9.8 kPa. Membrane fouling was assessed based on the required TMP, with mixed liquors characterised using capillary suction time, frozen image centrifugation and quantification of extracellular polymeric substances (EPS). SRT had a significant effect on these parameters: fouling was least at an SRT of 30 days and highest at 60 days, with some reduction as this extended to 90 days. Operation at SRT <30 days showed no further benefits. Although operation at a short SRT was optimal for membrane performance it led to lower specific methane productivity, higher biomass yields and higher effluent COD. Short SRT may also have accelerated the loss of essential trace elements, leading to reduced performance under these conditions. A COD-based mass balance was conducted, including both biomass and methane dissolved in the effluent.

Understanding the formation of recalcitrant dissolved organic nitrogen as a result of thermal hydrolysis and anaerobic digestion of municipal sludge

This study for the first time investigated the turnover of dissolved organic nitrogen in lab-scale thermal hydrolysis pretreatment at different temperatures with and without mesophilic anaerobic digestion at a solids retention time of 15 days.

Impacts of Temperature and Solids Retention Time, and Possible Mechanisms of Biological Hydrolysis Pretreatment on Anaerobic Digestion

Anaerobic digestion (AD) has benefits in sludge management, energy recovery, and pathogen reduction. In order to better understand the mechanisms of biological hydrolysis (BH) pretreatment on AD, biochemical methane potential (BMP) and continuous stirred-tank reactor (CSTR) tests were utilized to compare untreated municipal combined sludge with pilot-scale BH pretreated sludge. During the BH process, there was 15%, 30%, and 33% (w/w) volatile solids (VS) reduction after BH at 42 °C (BH42) for 24, 48, and 72 h, respectively; under BH61 (42 °C for 36 h and 61 °C for 6 h), and there was 10% and 30% (w/w) overall VS reduction after 36-h and 42-h hydrolysis, respectively. BMP results showed that BH42-pretreated sludge had 22.6% enhancement of methane yield compared to untreated sludge, and BH61 pretreated sludge had 29.4% enhancement of methane yield. Both temperature and solids’ retention time (SRT) contributed to the enhanced AD performance within 36 h, while temperature played more important roles after 36-h BH pretreatment. CSTR tests confirmed the acceleration of anaerobic digestion by BH pretreatment, and higher enhancement was observed when SRT of anaerobic digestion was shorter than 16 days. Through a literature review of BH-related studies, the possible mechanisms were highlighted for further optimization on the scale-up systems in order to reduce carbon footprint and operating expenditure for wastewater treatment plants.

Pushing the limits of solids retention time for enhanced biological phosphorus removal: process characteristics and Accumulibacter population structure

Reducing the solids retention time (SRT) of the enhanced biological phosphorus removal (EBPR) process can increase organic carbon diversion to the sidestream for energy recovery, thereby realizing some of the benefits of the high rate activated sludge (HRAS) process. Determining the washout (i.e. minimum) SRT of polyphosphate accumulating organisms (PAOs), therefore, allows for simultaneous phosphorus and carbon diversion for energy recovery from EBPR systems. However, few studies have investigated the washout SRT of PAOs in real wastewater, and little is known of the diversity of PAOs in high rate EBPR systems. Here we demonstrate efficient phosphorus removal (83% orthophosphate removal) in a high rate EBPR sequencing batch reactor fed real primary effluent and operated at 20 °C. Stable operation was achieved at a total SRT of 1.8 ± 0.2 days and hydraulic retention time of 3.7–4.8 hours. 16S rRNA gene sequencing data demonstrated that Accumulibacter were the dominant PAO throughout the study, with a washout aerobic SRT between 0.8 and 1.4 days. qPCR targeting the polyphosphate kinase gene revealed that Accumulibacter clades IIA, IIB and IID dominated the PAO community at low SRT operation, while clade IA was washed out at the lowest SRT values.