DEVELOPMENT OF ISOLATION SCHEME AND BACTERIOLOGICAL IDENTIFICATION OF PSEUDOMONAS SYRINGAE BACTERIA AND ITS APPROBATION

The article presents results of studies on development of isolation scheme and bacteriological identification of Pseudomonas syringae bacteria and its approbation. The introduction of the article describes the objects of Pseudomonas syringae contamination - fruit trees and shrubs, agricultural plants, which proves the relevance of the research in the field of expanding of laboratory methods for identifying phytopathogenic microorganisms. The author’s bacteriological scheme includes the use of King B Medium (Pseudomonas F Agar; Pronadisa 1532) as a selective medium. Initially, the isolated bacteria are differentiated to Pseudomonas genus, the following paramters are studied: anaerobic fermentation, production of enzymes catalase, lecithinase, lipase; hydrolysis of starch and gelatin; fermentation of glucose and lactose, also, a test for maceration is put. The second stage of the research includes the study of the growth of bacterial cultures on meat-and-peptone agar at 41 ° C and at 5% of NaCl; oxidase production, arginine hydrolase; fermentation of mannose and sorbitol; formation of levan, hydrogen sulfide and indole, esculin, a hypersensitivity reaction is set. The determined parametres allow to type the representatives of the genus to Pseudomonas syringae species within 192 hours. During the research, a collection of 12 strains of Pseudomonas syringae bacteria was formed, isolated from 97 objects of phytosanitary supervision and identified according to the developed technique. The proposed bacteriological scheme allows to differentiate the above microorganisms on the basis of the analysis of 25 parameters. The application of a phage biological product as a diagnosticum (according to the Otto method) expands the spectrum of the analyzed biological properties of the isolated and identified Pseudomonas syringae bacteria.

Preparation of (S)-2-Phenylpropionic Acid by CaCl2/CMC Nanoparticles Immobilized Candida rugosa Lipase-Catalyzed Hydrolysis in Micro Aqueous Mixed Organic Solvent Systems

Candida rugosa lipase was immobilized in this study using CaCl2/CMC nanoparticles that yielded a lipase loading capacity of 127 mg/g, with better thermal stability and activity of 91.8%. The hydrolysis of racemic 2-phenylpropionic acid isopropyl ester by free and immobilized Candida rugosa lipase was investigated in the mixed organic-solvent composed of isooctane and methyl tert-butyl ether (9.5:0.5, V/V). The optimal conditions were 35 °C and pH 7.5 for free Candida rugosa lipase hydrolysis. We obtained (S)-2-phenylpropionic acid with 44.85% conversion, 95.75% enantiomeric excess and enantiomeric ratio of 112. The CaCl2/CMC nanoparticles immobilized Candida rugosa lipase possesses high enantioselectivity, with E = 237 at 40 °C and pH 7.5. It was efficiently reusable in four cycles and appropriately enhanced enantioselectivity within 120–240.

Effects of Chocolates Using Low Calorie Cocoa Butter Substitutes on Rat’s Plasma Profile and Determination of Sn-1,3 Position

The present study sought to determine the effectiveness of newly developed low calorie chocolate using modified fat through enzymatic interesterification process as cocoa butter substitute of male Sprague Dawley rats (n = 35) compared to cocoa butter as control. Body weight gains did not differ significantly (p>0.05) in all the test groups fed with chocolates which were using different types of fats. Triacylglycerol and cholesterol test showed no significant difference (p>0.05) for all treatment samples. in this study, a pancreatic lipase hydrolysis was performed to determine the fatty acid composition of the sn-2 position of the structure lipids.

The impact of growth stimulators and retardants on the utilization of reserve lipids by sunflower seedlings

Using growth stimulators and anti-gibberillin preparations of different chemical compounds, we created different pressures in the coordinate covalent bond systems during germination of sunflower seeds. Using gibberellin and treptolem growth stimulators and Paclobutrazol and Chlormequat-Chlorid anti-gibberillin preparations is an efficient method of reconstructing coordinate covalent bond during the germination of seeds of oil-bearing crops. It allows determination of the role of the hormone factor in the utilization of reserve lipids over the heterotrophic phase of plant development. Blocking the synthesis of gibberellins by retardants caused decrease in activity of lipase, hydrolysis of reserve lipids and the meristem, which resulted in decrease in the energy of germination. Compared to the control, the impact of gibberellin increased the content of butyric acid, and the impact of Paclobutrazol reduced the content of butyric acid. Linoleic acid showed exactly the opposite changes. In relation to the control, a significant increase in the content of non-saturated linoleic acid was observed in both variants of the experiment. Gibberellic acid stimulated and Paclobutrazol slowed the usage of free higher fatty acids for the process of morphogenesis.

Lipase-catalyzed modification of natural Sapium sebiferum oil-based polyol for synthesis of polyurethane with improved properties

Sapium sebiferum oil-based polyol was modified by lipase hydrolysis for primary alcohols and further synthesis of polyurethane with improved properties.