Epidemiological Study of Dengue Hemorrhagic Fever (DHF) With Increased Incidence of Serum Glutamic Pyruvic Transaminase (SGPT) Levels at Aura Syifa Hospital Kediri

Dengue fever virus is an infectious disease that can infect others if bitten by Aedes aegypti mosquito. The virus has the potential to attack cells in the hepar organs so that the hepar is inflamed, swollen, and liver function is disrupted, and there is severe bleeding. The increase in transaminase enzymes as well as hepatomegaly is a frequent sign in dbd sufferers, thus reinforcing the notion that the course of dengue hemorrhagic fever (DHF) disease can affect hepar or liver organs. The presence of such events will increase the level of Serum Glutamic Pyruvic Transaminase (SGPT) and Serum Glutamic Oxaloacetic Transaminase (SGOT) in dengue fever patients. The purpose of this study is to find out the epidemiological study of Dengue dengue fever with the increase in SGPT and SGOT levels in dengue fever patients at Aura Syifa Kediri Hospital. This research method uses descriptive research design and total sampling technique for 1 month. Samples obtained for 1 month amounted to 15 samples. The results showed that from a sample of 15 respondents as many as 3 samples (20%) for high SGPT levels above normal and normal results as many as 12 samples (80%). The conclusion of this study shows that the value of SGPT levels has increased by 20% and is still above normal, but still have to be alert to small values.

PENGARUH EKSTRAK ASHITABA TERHADAP KADAR SERUM GLUTAMIC OXALOACETIC TRANSAMINASE DAN SERUM GLUTAMIC PYRUVIC TRANSAMINASE PADA TIKUS DENGAN LUKA BAKAR

Background: Serum glutamic oxaloacetic transaminase (SGOT) and serum glutamic pyruvic transaminase (SGPT) are increased in tissue damage. Ashitaba contains many compounds that can reduce serum SGOT and SGPT levels. Objective: This study was proposed to prove that giving Ashitaba extract could reduce serum SGOT and SGPT levels in rats exposed to burn injury. Methods: This study used a posttest only control group design with 20 rats as samples. All research samples were treated with second-degree burns and were divided into 2 different groups, the treatment group (given Ashitaba extract 300 mg /kg BW) and the control group. The blood serum was analyzed for SGOT and SGPT test on the 2nd, 8th, and 14th days. For statistical analysis, the Wilcoxon test used to analyze the levels of serum SGOT and SGPT. Results: There was no significant difference in serum SGOT and SGPT levels between the control and treatment groups on the 2nd, 8th, and 14th days Conclusion: Ashitaba extract could not reduce serum SGOT and SGPT levels in rats exposed to burn injury.

The Hepatotoxic and Nephrotoxic Effects of Purwoceng (Pimpinella pruatjan Molk.) Roots Ethanol Extract Administration in Subchronic Dose

Purwoceng (Pimpinella pruatjan Molk) is an original Indonesian herb which is known to have an aphrodisiac effect. The active compounds in Purwoceng potentially have hepatotoxic and nephrotoxic effects.This study was aimed to analyze the effect of subchronic administration of Purwoceng roots ethanol extract to Wistar Strain of Rattus norvegicus rats. The method of this subchronic toxicity study was an experimental post test only with control group design. Forty male Rattus norvegicus were randomly divided into four groups and get 28-days treatment. Group A as control received aquadest and 1% CMC (carboxymethyl cellulose), group B, C, and D were given Purwoceng roots ethanol extract of 42, 84, and 168 mg/KgBW/day and 1% CMC. Parameters tested were the levels of urea, creatinine, SGPT (serum glutamic pyruvic transaminase), SGOT(serum oxaloacetic pyruvic transaminase), kidney and hepar histopatology.The results showed a statistically significant for the liver histopathological in group B, creatinine, urea, and kidney histopathology in group C. Our study concluded that subchronic administration of Purwoceng (Pimpinella pruatjan Molk) roots ethanol extract could induce hepatotoxicity at the 42 mg/KgBW/day dose level and nephrotoxicity at the 84 mg/KgBW/day dose level.

GAMBARAN KADAR SGPT ( SERUM GLUTAMIC PYRUVIC TRANSAMINASE ) PADA PEROKOK AKTIF DI USIA 17 - 25 TAHUN DENGAN LAMA MEROKOK < 10 TAHUN

Data WHO (World Health Organization) Indonesia menduduki peringkat ketiga sebagai negara perokok terbanyak. Rokok adalah hasil olahan tembakau dan dalam sebatang rokok mengandung 4000 bahan kimia yang sangat berbahaya dan tiga kandungan rokok yang paling berbahaya adalah tar, nikotin, dan karbon monoksida. Paparan asap rokok terus menerus menimbulkan berbagai penyakit seperti kerusakan fungsi hati. Hati merupakan organ penting yang berfungsi untuk melakukan proses metabolisme dan detoksifikasi. Kerusakan hati dapat diketahui dengan meningkatnya kadar SGPT dalam aliran darah. SGPT merupakan enzim yang banyak ditemukan pada sel hati serta efektif untuk mendiagnosis destruksi hepatoseluler. Penelitian ini bertujuan untuk mengetahui kadar SGPT perokok aktif pada usia 17 - 25 tahun dengan lama merokok <10 tahun. Penelitian ini merupakan penelitian deskriptif dengan desain penelitian Croos Sectional. Tekhnik pengambilan sampel adalah purposive sampling. Hasil penelitian ini menunjukan 11 sampel memiliki kadar SGPT yang normal termasuk perokok ringan – sedang dan 4 sampel yang mengalami peningkatan kadar SGPT yang termasuk kedalam kategori perokok berat. Berdasarkan penelitian yang telah dilakukan, dapat disimpulkan gambaran kadar SGPT perokok aktif pada usia 17 – 25 tahun dengan lama merokok <10 tahun bahwa perokok aktif dalam kategori ringan hingga sedang berada dalam batas normal, sedangkan perokok aktif kategori berat mengalami peningkatan kadar SGPT.Kata Kunci : kadar SGPT, perokok aktif, usia 17 – 25 tahun, lama merokok < 10 tahun

Evaluation of Hepatoprotective and Antioxidant Activity of Ethanolic Extract of Artabotrys zeylanicus Stem against Various Hepatotoxins Induced Hepatotoxicity in Albino Wister Rats

The objective of the present study was to investigate the antioxidant and hepatoprotective activity of ethanolic stem extract of Artabotrys zeylanicus against paracetamol (PCT), Ethanol (ETN) and Isoniazid and Rifampicin (IR) induced hepatotoxicity in Albino wister rats. Methodology: The material was dried in shade, they were powdered and extracted with ethanol. Preliminary Phytochemical tests were done. The hepatoprotective activity of the ethanol extract was assessed in Albino wister rats. PCT (3 g/kg), ETN (5 gm/kg) and IR (100 mg/kg) has enhanced the levels of various biochemical markers of hepatic damage like Serum Glutamic Oxaloacetic Trasaminase (SGOT), Serum Glutamic pyruvic transaminase (SGPT), Alkaline phosphatise (ALP), bilirubin. Antioxidant levels were tested in all the Hepatotoxins treated and untreated groups. Results: The various biochemical and Histopathological investigations done were Serum Glutamic Oxaloacetic Trasaminase (SGOT), Serum Glutamic pyruvic transaminase (SGPT), Alkaline phosphatise (ALP), Bilirubin, antioxidant activity by 1,1-diphenyl 2-picryl hydrazyl (DPPH), Nitro Blue Tetrazolium (NBT), Hyderogen peroxide (H2O2), lipid perioxidation, hyderoxil radical and nitric oxide. Treatment of ethanolic extract of stem of A. zeylanicus (100mg/kg, 200mg/kg and 400mg/kg body weight) has brought back the altered levels of biochemical markers to the near normal levels in the dose dependent manner. Ethanolic extract of A. zeylanicus were observed to inhibit oxidant stress with the maximum value of 71% and 62% at the concentration of 100 µg/mL. The crude ethanolic extract of A. zeylanicus had a calculated IC50 value of 62.2 and 63.25 μg/mL, which is nearly similar to the calculated IC50 value of the known antioxidant, ascorbic acid, ie 65.3 μg/mL. While the rats treated with AZ extract (100, 200 and 400 mg/kg) which were shown as reduction/absence of inflammatory cells, vascular congestion, cellular degeneration, necrosis and vacuoles. In contrast, the lower doses (100 mg/kg) of ethanolic extract of AZ stem shown low protection than at higher dose 400 mg/kg. Conclusion: Our findings suggested that A. zeylanicus ethanol stem extract possessed a potent antioxidant and hepatoprotective activity.

Studi Pengaruh Paparan Radiasi Gamma Dan Ekstrak Kulit Manggis (Garcinia Mangostana) Terhadap Gambaran Mikroskopis Dan Kadar Sgpt Organ Hepar Mencit (Mus Musculus)

<p><strong>Abstract: </strong>This study examines the effect of the use of gamma radiation in the health sector, one of which is the use of gamma rays in the radiotherapy process. Irradiation into the body can cause the emergence of free radicals in the body which can harm other organs around the irradiation target. This study aims to determine the effect of exposure to gamma radiation on the liver of mice and the hepatoprotective properties of mangosteen peel extract (Garcinia mangostana) in reducing the resulting cell damage. This study was conducted by giving mice mangosteen peel extract with 5 different doses from those given 40 minutes of radiation exposure with a total dose of 268 μSv for 14 days, then the SGPT (serum glutamic pyruvic transaminase) levels were measured and cell damage figures from histopathological preparations. The results showed that before being given mangosteen peel extract, total damage to hepatocyte cells was 47.34%. After being given mangosteen peel extract, total damage to hepatocyte cells was 23.42%. Meanwhile, the SGPT level decreased to 77.7 U/L from the initial level of 128.67 U/L. This proves that mangosteen peel extract has hepatoprotective properties that can reduce cell damage due to exposure to gamma radiation.</p><p class="AbstrakIndonesia"><strong>Abstrak: </strong>Penelitian ini mengkaji pengaruh penggunaan radiasi gamma di bidang kesehatan, salah satunya adalah penggunaan sinar gamma dalam proses radioterapi. Penyerapan energi radiasi ke dalam tubuh dapat menyebabkan munculnya radikal bebas dalam tubuh yang dapat merugikan organ lain di sekitar target penyinaran. Penelitian ini bertujuan untuk mengetahui pengaruh paparan radiasi gamma terhadap hepar mencit dan sifat hepatoprotektif ekstrak kulit manggis<em>(Garcinia mangostana)</em> dalam mengurangi kerusakan sel yang ditimbulkan. Penelitian ini dilakukan dengan memberikan ekstrak kulit manggis 5 dosis varian berbeda ke mencit dan diberi paparan radiasi selama 40 menit dengan dosis total 268 μSv selama 14 hari, kemudian diukur kadar SGPT <em>(serum glutamic pyruvic transaminase)</em> dan jumlah kerusakan sel dari <em>p</em><em>reparat</em>histopatologis. Hasil penelitian menunjukkan bahwa sebelum diberikan ekstrak kulit manggis, kerusakan total sel hepatosit sebesar 47,34%. Setelah diberikan ekstrak kulit manggis, kerusakan total sel hepatosit sebesar 23,42%. Sementara itu, kadar SGPT turun menjadi 77,7 U/L dari kadar awal 128,67 U/L. Hal ini membuktikan bahwa ekstrak kulit manggis memiliki sifat hepatoprotektif yang dapat mengurangi kerusakan sel akibat paparan radiasi gamma.</p>

Analisis Kadar SGPT (Serum Glutamic Pyruvic Transaminase) Dan Kholinesterase Pada Petani Sayur Di Desa Riang Gede, Kecamatan Penebel, Kabupaten Tabanan

Excessive users in the long term can cause damage to liver function qhich is indicated by the presence of SGPT levels that exceed the reference value and cholinesterase levels that are less than the reference value. The purpose of this study was to determine the levels of SGPT and kholinesterase in vegetable famers in Riang Gede Village, Penebel District, Tabanan Regency. This research is a descriptive study using a statistical test that displays the lowest, highest value, average test results and deviation standards to correct errors in the analysis process. Blood sampling is done on 30 vegetable farmers by using a sampling technique in the form of random sampling SGPT and kholinesterase levels measured using a photometer, the results of the study showed an average SGPT level of 17,0 ± 0,02 U/L, the highest level of 48,0 ± 0,05 U/L and the lowest of 9,0 ± 0,05 U/L. The average level of cholinesterase is 5,9 +0,02 kU/L, the highest level is 8,0 ± 0,05 kU/L and the lowest level is 3,5±0,05 kU/L.

Nagapashana Pishti - A Potent Cardio Tonic

Nagapashana (Serpentine), a hydrous silicate of Magnesium (Mg6 (Sio10) OH8), is an important mineral drug used in Ayurveda, often in the form of Pishti (fine powder of Nagapashana). It is a Hrudya Dravya (cardiotonic). It is particularly indicated in Hrud Dourbalyaa, a condition associated with weakness of cardiac muscles. Cardiovascular disease is a leading cause of death in developed and developing countries. The present study was conducted to evaluate the cardio tonic activity of Nagapashana Pishti on Wistar rats. In the in-vivo study, Doxorubicin (2mg/kg) for 7 days was used to induce cardiac damage and the cardio tonic effect of Nagapashana Pishti at a dose of 150 and 300 mg/kg.b.w, was compared with standard drug Digoxin. Biochemical assays like serum glutamic oxaloacetic transaminase (SGOT), Serum glutamic pyruvic transaminase (SGPT), lactate dehydrogenase (LDH), Total Cholesterol (TC), Triglyceride (TG) and Creatine kinase Monoenzyme B (CKMB) were done together with histopathology of heart tissue and ECG analysis. The in-vivo study revealed that Nagapashana Pishti (300mg/kg) was relatively more effective due to decreased QT and ST interval in ECG, significantly reduced levels of serum CKMB, SGOT, SGPT, TC and LDH and improvement in myocardial tissue.

Hepatoprotective and antioxidant effect of Adiantum lunulatum Burm. F. leaf in alcohol-induced rat model

ABSTRACTBackgroundAdiantum lunulatum Burm. F. leaf (AL) and its related species have been used traditionally for the treatment of various diseases.ObjectiveThe present study evaluated the hepatoprotective, and antioxidant activities of ethanolic extract of AL.Methodology and ResultThe hepatoprotective effect of AL was evaluated against ethanol-induced hepatotoxicity in rats. Administration of ethanol (2 g/kg) showed a significant biochemical and histological deterioration in the liver of experimental animals. Pretreatment with ethanolic extract of AL (250 and 500 mg/kg b.wt. p.o) significantly reduced the elevated levels of serum enzymes like serum glutamic-oxaloacetic transaminase (AST), serum glutamic-pyruvic transaminase (ALT), alkaline phosphatase (ALP), total protein, total bilirubin and reversed the hepatic damage in the liver which evidenced the hepatoprotective activity. The superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) level notably increased due to doses of AL.ConclusionThe results of the present study demonstrate that the ethanolic extract of AL possesses hepatoprotective and antioxidant activities.Graphical Abstract

Analysis of serum glutamic pyruvic transaminase and serum glutamic oxaloacetic transaminase levels in tuberculosis patients who are undergoing oat treatment in Kendari City General Hospital, Kota Kendari, Indonesia

In the treatment of tuberculosis (TB), the patient is generally directly given Anti Tuberculosis Drugs (Obat Anti Tuberculosis/OAT) without examining Serum Glutamic Pyruvic Transaminase (SGPT) and Serum Glutamic Oxaloacetic Transaminase (SGOT) to see whether or not there is liver damage before treatment. Because of the side effects by OAT, it is important to know how the condition of the liver function of TB patients who consume OAT in Kendari City General Hospital (Rumah Sakit Umum Daerah/RSUD) Kota Kendari by looking at SGOT and SGPT levels in order to provide maximum treatment to TB patients. The method of this research was descriptive analysis with cross sectional approach. The sample is all patients had previously been diagnosed with TB by a doctor based on medical records by in RSUD Kota Kendari after 1-2 months OAT treatment without any sign of liver injury before. Examination of SGOT and SGPT levels was performed on all samples in the Laboratory of RSUD Kota Kendari using clinical chemistry analyzer. The results of this study showed that 20% patients with TB had elevated levels of SGPT and SGOT with average SGOT is 51 U/L and SGPT is 42.5. The Inference of this study that there is an increase in SGOT and SGPT levels in patients who consume OAT 1-2 months in RSUD Kota Kendari. Elevated levels of SGPT and SGOT due to Anti Tuberculosis (OAT) drugs did not occur in all patients and only increased in 3 people, but qualitatively the effect of administering anti-tuberculosis drugs significantly increased levels of SGPT and SGPT. Thus, monitoring of liver physiology remains recommended especially for elderly patients.