STENOTROPHOMONAS MALTOPHILIA RESISTANCE AND BIODEGRADATION POTENTIAL

In Current research the main goal was to study the resistance plasmids stability causes in S. maltophilia native strains. Using some microbiological, biochemical and genetical methods, it was noted, that the resistance to 13 mostly used in medicine, veterinary and agriculture antibiotics is stable and can be transmitted, even after the long-time cultivation without contact with any antibiotic. Then, using PCR, transformation analyses and xenobiotic tests, it was shown nucleoid and plasmid localization of studied genes. Polyphenol oxidases and caseinase genes were detected on nucleoid, while the lipases genes were indicated both on nucleoid and plasmids. It was found that biodegradation genes define the stabile replication of antibiotic resistance plasmids in a majority of S. maltophilia studied strains

STUDY OF RESISTANCE TO ANTIBIOTICS OF MICROORGANISMS ISOLATED FROM ANTARCTIC CLIFFS AND BLACK SEA BOTTOM SEDIMENTS

Resistance to antibiotics of bacteria isolated from extreme ecosystems was compared. Pseudomonas аntarctica and Rothia sp. strains isolated from rocky lichen of Antarctic Galindez Island were highly resistant to the antibiotics. Rhodococcus fascians 181n3, Sporosarcina aquamarina 188n2 and Staphylococcus epidermidis 190n1 strains were found to be sensitive to all antibiotics studied. Plasmid localization of genes coding resistance to Cr (VI) and Co2+ was determined. Correlation of resistance of microorganisms isolated from «ecologically pure» ecosystem to antibiotics demonstrated in this study and resistance of these microorganisms to the toxic metals exhibited by us earlier is fundamentally new data which testify the phenomenon of simultaneous resistance of microbial community to different extremal factors.