Heavy Metal Bioremediation by Novel Microbial Strains Proteus Mirabilis and Bordetella Avium

The entitled study focuses on exploring the microbial diversity and its applicability in the remediation of metal contaminated soil using microbes which is a reliable, and cost effective technique. Present investigation studied microbiota present in tungsten enriched soil of kuhi-Agargaon-Khobna region using culturable approach confirmed by ICP-MS and SEM-EDS analysis. Furthur applicability in Bioremediation and Azo Dye degradation was studied. XRF analyses of soil samples were performed followed by traditional microbiological analysis for isolation of tungsten tolerant bacteria’s. Tungsten accumulation was confirmed using ICP-MS and SEM-EDS techniques. Different metals and azo dye tolerance studies were conducted. Antibiotic susceptibility test revealed the antibiotic resistance profile of these microbes. XRF analyses of soil samples from these regions measured about 300 ppm tungsten/kg of soil at the Agargaon region and 200 ppm tungsten/kg of soil at the Khobna region. The traditional microbiological analysis resulted in two bacterial isolates which were identified for tolerance to tungsten named as Proteus mirabilis strain RS2K and Bordetella avium strain RS3K through 16S rDNA gene sequencing and phylogenetic analysis. These microbes were found to accumulate tungsten intracellularly as confirmed through ICP-MS and SEM-EDS analyses. In addition to (sodium) tungsten, the microbes were tolerant to tungstic acid, ammonium metaparatungstate, mercuric chloride, cobalt chloride and azo dyes .Microbes exhibited well-equipped cellular mechanisms for metal tolerance to survive in heavy metal-laden ecology. The novel strains obtained through a culturable approach in this study contain substantial potential in bioleaching of heavy metals and green mining.

Bordetella avium-associated endophthalmitis: case report

Background Bordetella avium, an aerobic bacterium that rarely causes infection in humans, is a species of Bordetella that generally inhabits the respiratory tracts of turkeys and other birds. It causes a highly contagious bordetellosis. Few reports describe B. avium as a causative agent of eye-related infections. Case presentation We report a case of acute infectious endophthalmitis associated with infection by B. avium after open trauma. After emergency vitrectomy and subsequent broad-spectrum antibiotic treatment, the infection was controlled successfully, and the patient’s vision improved. Conclusions B. avium can cause infection in the human eye, which can manifest as acute purulent endophthalmitis. Nanopore targeted sequencing technology can quickly identify this organism. Emergency vitrectomy combined with lens removal and silicone oil tamponade and the early application of broad-spectrum antibiotics are key for successful treatment.