Quantitative Analysis Of Trace Metal In Water And Soft Tissues Of Balanus Amphitrite In Nandikadal Lagoon, Sri Lanka

Due to long–term military activities carried out in Nandikadal lagoon situated at Mullaitivu, Sri Lanka, there are high possibilities to impact water and biota with heavy metals. Balanus amphitrite (Barnacles) has been found as a strong candidate for biomonitoring of trace metals. Therefore, the present study describes the impacts of trace metal pollution on water and the soft tissues of Balanus amphitrite. Water samples and barnacles were collected from two locations of the lagoon for four months during the dry and wet seasons. Quantitative analysis of trace metals (Cd, Cu, Cr, Pb and Zn) were detected in the water and tissue samples using flame atomic absorption spectrometry. Cd, Cr, Pb and Cu found in tissues of barnacles were several times higher than the International recommended level except for Zn. The concentration of Pb in tissues of barnacles showed a significant (p < 0.05) spatial variation. The Cd and Cu concentrations obtained in soft tissues showed significant (p < 0.05) seasonal variations. The concentration of Cr in water showed a significant (p < 0.05) spatial variation and other trace metals (Pb,Cd) except Zn, showed significant (p < 0.05) seasonal variations. There were significant positive correlations between water and soft tissues with respect to Cd (p=0.000 & r = 0.893) and Pb (p=0.002 & r=0.435). Zn found in samples showed negative correlation between water and soft tissues. According to this results Balanus amphitrite has an ability to accumulate trace metals several times greater than in the lagoon water. Therefore, Balanus amphitrite is a strong net–accumulator of Cd, Pb, Zn, Cu, Cr. Hence, it is a good choice to be used as a bioindicator organism to find the level impact of trace metal contamination.

Environmentally Friendly Antifouling Metabolites from Red Sea Organisms

Seventy-one marine organisms representing different classes of marine fauna and flora were collected from the Red Sea. They include sponges, hydrozoan, soft corals, sea cucumber, ascidian, cyanobacteria, and macroalgae. The methanolic extracts were evaluated for their toxicity and settlement inhibition effects by using cultured Balanus amphitrite. Thirty-three extracts displayed antifouling effects: four samples were highly potent at 1 μg/mL with a percentage of settlement inhibition above 31%, twenty-two were potent at 10 μg/mL with a percentage of settlement inhibition between 16 and 30%, and seven were active at 10 μg/mL with a percentage of settlement inhibition between 0 and 15%. Two promising extracts were purified by employing several chromatographic techniques, leading to the isolation of 12 known compounds. The isolated compounds were evaluated for their antifouling activities and demonstrated potent antifouling effects with EC50 values of less than 10 μg/mL.

Designing a Laboratory Bioassay for Evaluating the Efficacy of Antifouling Paints on Amphibalanus amphitrite Using a Flow-Through System

With the aim of establishing a protocol for evaluating the efficacy of antifouling paints on different organisms, a flow-through laboratory test using triangular boxes was developed for cyprids of the barnacle Amphibalanus (=Balanus) amphitrite. Six different formulations of antifouling paints were prepared in increasing content (0 to 40 wt.%) of Cu2O, which is the most commonly used antifouling substance, and each formulation of paint was coated on one surface of each test plate. The test plates were aged for 45 days by rotating them at a speed of 10 knots inside a cylinder drum with continuously flowing seawater. The settlement behavior of 3-day-old cyprids released inside triangular boxes made from the test plates was observed. A decreasing number of juveniles settled on surfaces of test plates that were coated with paint containing more than 30 wt.% of Cu2O. Results of the laboratory bioassays were consistent with those from the field experiments.

HAMA MANGROVE DI KECAMATAN BATU AMPAR, KABUPATEN KUBU RAYA, KALIMANTAN BARAT Mangrove Pests at Batu Ampar, Kubu Raya, West Kalimantan

Mangrove ecosystem is a complex tropical forest ecosystem that is able to live and to adapt in coastal environments.Unfortunatelly, its existence is vulnerable to the environmental change. Unless utilization of mangrove forest is notfollowed by enrichment and rehabilitation, it will lead to degradation of logged-over land such as pest and diseaseinfestation. The pest study was conducted in mangrove stands in PT. Bina Ovivipari Semesta (BIOS), Kubu Raya, WestKalimantan. The result of study consisted of three sample plots obtained from the average of mangrove pest on thecutting area of 2017 (93.33%), attack intensity (28%). Additionally, the average of cutting area of 2012 were at 91.95%and attack intensity (29%) while the protection forest obtained the average of attack at 79.23% and attack intensity(21.87%). The results of pest identification of mangrove arboretum were 9 pests, including pagoda bagworm (Pagodiellaspp.), bagworm (Acanthopsyche sp.), krama / tree climbing crab (Episesarma spp.), nacerdes beetle (Xanthochroa sp.),aphid (Prociphilus tessellatus), barnacles (Balanus amphitrite), snails (Gastropoda Class), cobwebs, leatherleaf slugs(Vaginula bleekeri).Key words: Kubu Raya, Mangrove, Pagodiella spp., PT. BIOS